scholarly journals Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid

2010 ◽  
Vol 6 ◽  
Author(s):  
Arne Homann ◽  
Riaz-ul Qamar ◽  
Sevnur Serim ◽  
Petra Dersch ◽  
Jürgen Seibel
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Carcinoma Cell ◽  
Larynx Carcinoma ◽  
Interaction Processes ◽  
Acid Analogue ◽  
Human Larynx ◽  
Sialic Acid Analogue ◽  
Metabolic Glycoengineering ◽  
Human Larynx Carcinoma

Sialic acids are located at the termini of mammalian cell-surface glycostructures, which participate in essential interaction processes including adhesion of pathogens prior to infection and immunogenicity. Here we present the synthesis and bioorthogonal metabolic incorporation of the sialic acid analogue N-(1-oxohex-5-ynyl)neuraminic acid (Neu5Hex) into the cell-surface glycocalyx of a human larynx carcinoma cell line (HEp-2) and its fluorescence labelling by click chemistry.

scholarly journals Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System

2011 ◽  
Vol 8 (3) ◽  
pp. 728-735
Author(s):  
Baghdad Science Journal
Keyword(s):  
Cell Line ◽  
High Efficiency ◽  
Carcinoma Cell ◽  
Transfection Efficiency ◽  
Carcinoma Cell Line ◽  
Larynx Carcinoma ◽  
Human Larynx ◽  
Therapy Studies ◽  
Human Larynx Carcinoma ◽  
Transfection Agent

Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently.

Characterization of human larynx carcinoma cell lines HLaC′79 and HLaC′82: a common origin but diverged malignancies

1995 ◽  
Vol 13 (5) ◽  
pp. 319-327 ◽  
Author(s):  
Anna M. Tomson ◽  
Janny Scholma ◽  
Trijnie Dijkhuizen ◽  
André P. M. Wijnakker ◽  
Miriam van der Werf ◽  
...  
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Common Origin ◽  
Larynx Carcinoma ◽  
Carcinoma Cell Lines ◽  
Human Larynx ◽  
Human Larynx Carcinoma

Total Biosynthesis of Legionaminic Acid, a Bacterial Sialic Acid Analogue

2016 ◽  
Vol 55 (39) ◽  
pp. 12018-12021 ◽  
Author(s):  
Mohamed I. Hassan ◽  
Benjamin R. Lundgren ◽  
Michael Chaumun ◽  
Dennis M. Whitfield ◽  
Brady Clark ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Acid Analogue ◽  
Sialic Acid Analogue

Synthesis of the First Sialic Acid Analogue Containing a y-Pyrone Moiety

1999 ◽  
Vol 52 (12) ◽  
pp. 1127 ◽  
Author(s):  
Hua Chee Ooi ◽  
B Sebastian ◽  
M. Marcuccio ◽  
W. Roy Jackson ◽  
David F. O'Keefe
Keyword(s):  
Sialic Acid ◽  
Acid Derivative ◽  
Acetylneuraminic Acid ◽  
Acid Analogue ◽  
Sialic Acid Analogue

Oxidation of the two DANA (2-deoxy-2,3-didehydro-N-acetylneuraminic acid) derivatives (3) and (4) by using several reagents has led to the isolation of the first sialic acid derivative (1) containing a γ-pyrone moiety.

ChemInform Abstract: Synthesis of a Carbocyclic Sialic Acid Analogue for the Inhibition of Influenza Virus Neuraminidase.

ChemInform ◽  
2010 ◽  
Vol 32 (36) ◽  
pp. no-no
Author(s):  
Armandodoriano Bianco ◽  
Mario Brufani ◽  
Fedele Manna ◽  
Cristiana Melchioni
Keyword(s):  
Influenza Virus ◽  
Sialic Acid ◽  
Acid Analogue ◽  
Sialic Acid Analogue ◽  
Influenza Virus Neuraminidase
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