scholarly journals Cell lineage distribution atlas of the human stomach reveals heterogeneous gland populations in the gastric antrum

Gut ◽  
2014 ◽  
Vol 63 (11) ◽  
pp. 1711-1720 ◽  
Author(s):  
Eunyoung Choi ◽  
Joseph T Roland ◽  
Brittney J Barlow ◽  
Ryan O'Neal ◽  
Amy E Rich ◽  
...  
Keyword(s):  
Cell Lineage ◽  
Human Stomach ◽  
Gastric Antrum ◽  
Distribution Atlas ◽  
Lineage Distribution

scholarly journals Haematopoietic cell lineage distribution of MPLW515L/K mutations in patients with idiopathic myelofibrosis

2007 ◽  
Vol 137 (4) ◽  
pp. 378-379 ◽  
Author(s):  
Wen-Yang Hu ◽  
Yan Zhao ◽  
Takefumi Ishii ◽  
Selcuk Sozer ◽  
Jun Shi ◽  
...  
Keyword(s):  
Cell Lineage ◽  
Idiopathic Myelofibrosis ◽  
Haematopoietic Cell ◽  
Lineage Distribution

scholarly journals Neuropeptide W is present in antral G cells of rat, mouse, and human stomach

2006 ◽  
Vol 188 (1) ◽  
pp. 49-57 ◽  
Author(s):  
M S Mondal ◽  
H Yamaguchi ◽  
Y Date ◽  
K Toshinai ◽  
T Kawagoe ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
Light And Electron Microscopy ◽  
Reversed Phase ◽  
Human Stomach ◽  
Gastric Antrum ◽  
Serum Prolactin ◽  
Dark Phase ◽  
Electron Microscopic ◽  
G Cells ◽  
Systemic Vein

Neuropeptide W (NPW) is a 30-amino-acid peptide initially isolated from the porcine hypothalamus as an endogenous ligand for the G protein-coupled receptors GPR7 and GPR8. An intracerebroventricular administration of NPW increased serum prolactin and corticosterone concentrations, decreased dark-phase feeding, raised energy expenditure, and lowered body weight. Peripherally, GPR7 receptors are abundantly expressed throughout the gastrointestinal tract; the presence of NPW in the gastrointestinal endocrine system, however, remains unstudied. Using monoclonal and polyclonal antibodies raised against rat NPW, we studied the localization of NPW in the rat, mouse, and human stomach by light and electron microscopy. NPW-immunoreactive cells were identified within the gastric antral glands in all three species. Double immunohistochemistry and electron-microscopic immunohistochemistry studies in rats demonstrated that NPW is present in antral gastrin (G) cells. NPW immunoreactivity localized to round, intermediate-to-high-density granules in G cells. NPW-immunoreactive cells accounted for 90% chromagranin A- and 85% gastrin-immunoreactive endocrine cells in the rat gastric antral glands. Using reversed-phase HPLC coupled with enzyme immunoassays specific for NPW, we detected NPW30 and its C-terminally truncated form, NPW23, in the gastric mucosa. Plasma NPW concentration of the gastric antrum was significantly higher than that of the systemic vein, suggesting that circulating NPW is derived from the stomach. Plasma NPW concentration of the gastric antrum decreased significantly after 15-h fast and increased after refeeding. This is the first report to clarify the presence of NPW peptide in the stomachs of rats, mice, and humans. In conclusion, NPW is produced in gastric antral G cells; our findings will provide clues to additional mechanisms of the regulation of gastric function by this novel brain/gut peptide.

Depletion of Intestinal Stem Cell Niche Factors Contributes to the Alteration of Epithelial Differentiation in SAMP1/YitFcsJ Mice With Crohn Disease-Like Ileitis

2020 ◽  
Author(s):  
Chansu Lee ◽  
Sung Noh Hong ◽  
Eun Ran Kim ◽  
Dong Kyung Chang ◽  
Young-Ho Kim
Keyword(s):  
Crohn Disease ◽  
Formation Rate ◽  
Paneth Cell ◽  
Cell Lineage ◽  
Epithelial Differentiation ◽  
Terminal Ileitis ◽  
Differentiated Cells ◽  
Cell Niche ◽  
Akr Mice ◽  
Lineage Distribution

Abstract Background SAMP1/YitFcsJ (SAMP1) mice spontaneously develop terminal ileitis resembling human Crohn disease. SAMP1 mice have exhibited alteration of epithelial cell lineage distribution and an overall proliferation of the crypt cell population; however, it has not been evaluated whether epithelial differentiation is impaired because of dysfunction of intestinal stem cells (ISCs) or their niche factors. Methods Using the intestine of SAMP1 mice aged 10 to 14 weeks, morphometric alterations in the crypt-villus architecture, ISCs, crypt cells, and differentiated cells; organoid formation capacity of intestinal crypts; and niche signaling pathways were analyzed and compared with those of age-matched control AKR/J (AKR) mice. Results The ileum of SAMP1 mice showed increased depth of intestinal crypts and decreased surface area of the villi compared with those in the ileum of AKR mice. The number of ISCs in the ileal crypts did not differ between SAMP1 and AKR mice; however, the number of Paneth cells decreased and the number of transient amplifying cells increased. The organoid formation rate of the ileal crypts of SAMP1 mice decreased significantly compared with that of AKR mice. The performance of RNA sequencing for intestinal crypts found that the expression of ISC niche factors, such as Wnt3, Dll1, and Dll4, was decreased significantly in the ileal crypts of SAMP1 mice compared with those of AKR mice. Among the ISC niche signals, the Notch signaling-related genes tended to be downregulated. In particular, immunocytochemistry revealed that the expression of Paneth cell–expressing Notch ligand Dll4 was significantly decreased in the intestinal tissue and organoids of SAMP1 mice compared with those of AKR mice. Conclusions Depletion of niche factors for ISCs contributes to the alteration of epithelial differentiation in SAMP1 mice.

Excluded Gastric Antrum Simulating the Zollinger-Ellison Syndrome

1964 ◽  
Vol 47 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Brian A. Scobie ◽  
Douglas B. McGill ◽  
James T. Priestley ◽  
Randolph A. Rovelstad
Keyword(s):  
Gastric Antrum ◽  
Zollinger Ellison Syndrome
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