Effect of GNAS transcript manipulation on human mesenchymal stem cells differentiation towards osteocyte cell lineage: insight into the pathophysiology of ectopic ossification in GNAS-related disorders

2015 ◽  
Author(s):  
Francesca Marta Elli ◽  
Valentina Boldrin ◽  
Valentina Parazzi ◽  
Enrico Ragni ◽  
Paolo Bordogna ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Lineage ◽  
Human Mesenchymal Stem Cells ◽  
Ectopic Ossification ◽  
Insight Into

scholarly journals Analyzing Metabolic States of Adipogenic and Osteogenic Differentiation in Human Mesenchymal Stem Cells via Genome Scale Metabolic Model Reconstruction

2021 ◽  
Vol 9 ◽  
Author(s):  
Thora Bjorg Sigmarsdottir ◽  
Sarah McGarrity ◽  
James T. Yurkovich ◽  
Óttar Rolfsson ◽  
Ólafur Eysteinn Sigurjónsson
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Potential ◽  
Cell Lineage ◽  
Human Mesenchymal Stem Cells ◽  
Metabolic Model ◽  
Metabolic Function ◽  
Metabolic States ◽  
Initial Discovery ◽  
Genome Scale

Since their initial discovery in 1976, mesenchymal stem cells (MSCs) have been gathering interest as a possible tool to further the development and enhancement of various therapeutics within regenerative medicine. However, our current understanding of both metabolic function and existing differences within the varying cell lineages (e.g., cells in either osteogenesis or adipogenesis) is severely lacking making it more difficult to fully realize the therapeutic potential of MSCs. Here, we reconstruct the MSC metabolic network to understand the activity of various metabolic pathways and compare their usage under different conditions and use these models to perform experimental design. We present three new genome-scale metabolic models (GEMs) each representing a different MSC lineage (proliferation, osteogenesis, and adipogenesis) that are biologically feasible and have distinctive cell lineage characteristics that can be used to explore metabolic function and increase our understanding of these phenotypes. We present the most distinctive differences between these lineages when it comes to enriched metabolic subsystems and propose a possible osteogenic enhancer. Taken together, we hope these mechanistic models will aid in the understanding and therapeutic potential of MSCs.

An insight into the whole transcriptome profile of four tissue-specific human mesenchymal stem cells

2019 ◽  
Vol 14 (9) ◽  
pp. 841-865 ◽  
Author(s):  
Dana Alhattab ◽  
Fatima Jamali ◽  
Dema Ali ◽  
Hana Hammad ◽  
Sofia Adwan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Immune Modulation ◽  
Human Mesenchymal Stem Cells ◽  
Human Mscs ◽  
Transcriptome Profile ◽  
Bone Marrow Adipose Tissue ◽  
Different Origins ◽  
Whole Transcriptome ◽  
Insight Into

Aim: Variations in the clinical outcomes using mesenchymal stem cells (MSCs) treatments exist, reflecting different origins and niches. To date, there is no consensus on the best source of MSCs most suitable to treat a specific disease. Methods: Total transcriptome analysis of human MSCs was performed. MSCs were isolated from two adult sources bone marrow, adipose tissue and two perinatal sources umbilical cord and placenta. Results: Each MSCs type possessed a unique expression pattern that reflects an advantage in terms of their potential therapeutic use. Advantages in immune modulation, neurogenesis and other aspects were found. Discussion: This study is a milestone for evidence-based choice of the type of MSCs used in the treatment of diseases.

scholarly journals A Novel Human Artificial Chromosome Vector Provides Effective Cell Lineage-Specific Transgene Expression in Human Mesenchymal Stem Cells

Stem Cells ◽  
2005 ◽  
Vol 23 (10) ◽  
pp. 1608-1616 ◽  
Author(s):  
Xianying Ren ◽  
Motonobu Katoh ◽  
Hidetoshi Hoshiya ◽  
Akihiro Kurimasa ◽  
Toshiaki Inoue ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transgene Expression ◽  
Cell Lineage ◽  
Human Mesenchymal Stem Cells ◽  
Artificial Chromosome ◽  
Human Artificial Chromosome

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

The Effect of Link N on the Differentiation of Human Mesenchymal Stem Cells

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1320001-s-0032-1320001
Author(s):  
F. Mwale ◽  
H. T. Wang ◽  
L. Haglund ◽  
P. J. Roughley ◽  
J. Antoniou
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

Generation of human mesenchymal stem cells lacking diabetoporosity-associated miRNAs using CRISPR/Cas9

2020 ◽  
Author(s):  
I Foessl ◽  
A Groselj-Strele ◽  
JC Piswanger-Sölkner ◽  
H Dobnig ◽  
A Fahrleitner-Pammer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
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