An evaluation of Neisseria gonorrhoeae antimicrobial susceptibility testing in the UK

The only method currently available to perform Neisseria gonorrhoeae antimicrobial susceptibility testing (Ng-AST) requires a viable organism obtained by culture. Reports of in vitro resistance to extended-spectrum cephalosporins, the treatment of choice for gonorrhoea, coupled with increasing gonorrhoea diagnoses is worrying. The aim of this study was to identify various methodologies employed by the UK microbiology laboratories to perform Ng-AST. Of the 118 laboratories that responded, 114 offered Ng-AST; the majority (82.5%, 94/114) of the laboratories used British Society for Antimicrobial Chemotherapy methodology for Ng-AST. The other main findings were infrequent use of quality control procedures and inconsistent susceptibility testing of the antibiotics used routinely for treatment.

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Antimicrobial susceptibility testing of Neisseria gonorrhoeae and implications for epidemiology and therapy.

Clinical Microbiology Reviews ◽

10.1128/cmr.6.1.22 ◽

1993 ◽

Vol 6 (1) ◽

pp. 22-33 ◽

Cited By ~ 14

Author(s):

T Fekete

Keyword(s):

Neisseria Gonorrhoeae ◽

Antimicrobial Susceptibility ◽

Antimicrobial Agents ◽

Susceptibility Testing ◽

The United States ◽

Primary Treatment ◽

Antimicrobial Susceptibility Testing ◽

Response To Therapy ◽

In Vitro Tests

Antimicrobial susceptibility testing (AST) of Neisseria gonorrhoeae has been under development since the early days of antimicrobial agents. However, it is rarely applied to clinical isolates today. The history of the various in vitro tests to determine the susceptibility of N. gonorrhoeae to antibiotics is rich with evidence that these results predict response to therapy for almost all agents tested. Further, AST is a useful and important aspect of strain characterization and disease epidemiology in conjunction with the more specific but laborious techniques of auxotyping, serotyping, and plasmid analysis. Current technology has overcome many of the objections to AST for N. gonorrhoeae with standardization of test media and the development of an accurate disk diffusion AST method that is suited to most clinical laboratories regardless of volume or level of technical expertise. Ironically, the very low level of resistance to the current primary treatment strategy in the United States, ceftriaxone or another potent cephalosporin, makes the use of AST somewhat superfluous.

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Analysis of antimicrobial susceptibility testing methods and variables and in vitro activity of gepotidacin against urogenital Neisseria gonorrhoeae in men

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2021.115484 ◽

2021 ◽

pp. 115484

Author(s):

Nicole E. Scangarella-Oman ◽

Paula Dixon ◽

Laura M. Koeth ◽

Jeanna DiFranco-Fisher ◽

Linda A. Miller

Keyword(s):

Neisseria Gonorrhoeae ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Antimicrobial Susceptibility Testing ◽

Vitro Activity ◽

In Vitro Activity ◽

Testing Methods

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EPIDEMILOGICAL ANALYSIS OF NEISSERIA GONORRHOEAE ISOLATES BY ANTIMICROBIAL SUSCEPTIBILITY TESTING, AUXOTYPING AND SEROTYPING

Indian Journal of Medical Microbiology ◽

10.1016/s0255-0857(21)02110-1 ◽

2007 ◽

Vol 25 (3) ◽

pp. 225-229

Author(s):

P Khaki ◽

P Bhalla ◽

P Sharma ◽

R Chawla ◽

K Bhalla

Keyword(s):

Neisseria Gonorrhoeae ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Antimicrobial Susceptibility Testing

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Gentamicin susceptibility among a sample of multi-drug resistantNeisseria gonorrhoeaeisolates in India

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01907-16 ◽

2016 ◽

pp. AAC.01907-16 ◽

Cited By ~ 1

Author(s):

Manju Bala ◽

Vikram Singh ◽

Aradhana Bhargava ◽

Monika Kakran ◽

Naveen Chandra Joshi ◽

...

Keyword(s):

Effective Treatment ◽

Treatment Outcomes ◽

Treatment Option ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Dual Therapy ◽

Clinical Treatment ◽

Antimicrobial Susceptibility Testing ◽

Effective Treatment Option

Antimicrobial susceptibility testing of 258N. gonorrhoeaeisolates by Etest determined that 60.1% were MDR while 5% strains had decreased susceptibility to currently recommended extended-spectrum cephalosporins (ESCs). Among these, 84.5% MDR and 76.9% strains having decreased susceptibility to ESCs were susceptible to gentamicin. No MDR isolate was resistant to gentamicin. Thesein vitroresults suggest that gentamicin might be an effective treatment option for the MDR strains and in dual therapy for gonorrhea. However, further research regarding the clinical treatment outcomes is essential.

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