HBME-1 is expressed by erythroid precursors in early maturation stage and can be a valuable tool for evaluation of dyserythropoiesis in bone marrow core biopsy specimens

2016 ◽  
Vol 69 (10) ◽  
pp. 933-937 ◽  
Author(s):  
Riad Arana ◽  
Daniel Lusina ◽  
Thorsten Braun ◽  
Rémi Letestu ◽  
Claude Gardin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Core Biopsy ◽  
Maturation Stage ◽  
Early Maturation ◽  
Biopsy Specimens ◽  
Erythroid Precursors

Loss of Membrane Expression of E-Cadherin in Leukemic Erythroblasts

2001 ◽  
Vol 125 (2) ◽  
pp. 198-201
Author(s):  
Geza Acs ◽  
Virginia A. LiVolsi
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Biopsy ◽  
Bone Marrow Aspirate ◽  
Hematopoietic Cells ◽  
Immunoperoxidase Staining ◽  
Membrane Expression ◽  
Biopsy Specimens ◽  
Erythroid Precursors ◽  
Adhesive Interactions ◽  
E Cadherin

Abstract Context.—The special societal relationships existing between various cell types in bone marrow suggests that there may be a link between the adhesive characteristics of hematopoietic cells and their maturation. Egress of the developing hematopoietic cells is also a highly regulated process governed by adhesive interactions. In leukemia, immature blasts are not retained within the marrow, suggesting a breakdown of adhesive mechanisms. Recent reports suggest that E-cadherin, an epithelial adhesion molecule, is expressed on erythroid precursors and megakaryocytes, but not on other hematopoietic marrow elements. Objective.—To characterize the expression pattern of E-cadherin in normal and leukemic erythroid precursors by immunohistochemistry in paraffin-embedded tissue and bone marrow aspirate smears. Methods.—Five normal bone marrow specimens from rib resections, 15 trephine bone marrow biopsy specimens, and 6 bone marrow aspirate smears from the iliac crest of patients with no known leukemia were selected. Fourteen bone marrow biopsy specimens from patients with erythroleukemia were also studied. Immunoperoxidase staining of paraffin-embedded tissue and air-dried aspirate smears for E-cadherin (1:200 dilution, HECD-1 clone) was performed using the avidin-biotin peroxidase technique. Results.—In paraffin-embedded bone marrow biopsy and rib specimens and in air-dried bone marrow aspirate smears, strong membrane expression of E-cadherin was seen in the normal erythroid precursors in all cases. In contrast, no membrane expression of E-cadherin was present in any of the bone marrow biopsy specimens from patients with erythroleukemia. Conclusions.—Immunohistochemical detection of membrane expression of E-cadherin may be a useful tool for identification of erythroid precursors. Cells of erythroleukemia lack membrane expression of E-cadherin, in contrast to their normal counterparts. Further studies are needed to define the potential role of E-cadherin in the maturation of erythroid precursors and to ascertain the significance of loss of membrane expression of E-cadherin in erythroleukemia.

scholarly journals Bone Marrow Core Biopsy Specimens in AL (Primary) Amyloidosis

2003 ◽  
Vol 120 (4) ◽  
pp. 610-616 ◽  
Author(s):  
Niall Swan ◽  
Martha Skinner ◽  
Carl J. O’Hara
Keyword(s):  
Bone Marrow ◽  
Core Biopsy ◽  
Primary Amyloidosis ◽  
Biopsy Specimens

Flow Cytometric Immunophenotyping of Bone Marrow Core Biopsies

1999 ◽  
Vol 123 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Cherie H. Dunphy ◽  
Frank R. Dunphy ◽  
John L. Visconti
Keyword(s):  
Bone Marrow ◽  
Core Biopsy ◽  
Hematologic Malignancy ◽  
Data Extraction ◽  
Final Diagnosis ◽  
Hematologic Malignancies ◽  
Cell Of Origin ◽  
Flow Cytometric Immunophenotyping ◽  
Flow Cytometric ◽  
Biopsy Specimens

Abstract Objective.—To report a method for flow cytometric immunophenotyping (FCI) bone marrow (BM) core biopsies in patients with hematologic malignancies of the BM who present with a failed BM aspiration (“dry tap”). Design and Setting.—Core biopsy specimens of BM were obtained from 8 patients who presented with previously undiagnosed hematologic malignancies arising in (7 cases) or secondarily involving (1 case) the BM and a dry tap. Suspensions of the BM core biopsy specimens were prepared and analyzed by FCI methods. Data Extraction and Data Synthesis.—The FCI data were analyzed in conjunction with cytomorphologic, histologic, immunohistochemical, and cytogenetic findings in all cases to determine a final diagnosis. Conclusions.—The prepared BM core suspensions were viable and allowed for a complete immunophenotype profile by FCI in all cases, resulting in a clear definition of the cell of origin of the hematologic malignancy. Because of lack of preservation of architectural features and the potential for artifactual alterations of the relative frequency of abnormal cells, the FCI data must always be correlated with histologic sections of the BM biopsy.

Immunohistochemistry for PAX7 is a useful confirmatory marker for Ewing sarcoma in decalcified bone marrow core biopsy specimens

2018 ◽  
Vol 473 (6) ◽  
pp. 765-769 ◽  
Author(s):  
Sebastian Fernandez-Pol ◽  
Matt van de Rijn ◽  
Yasodha Natkunam ◽  
Gregory W. Charville
Keyword(s):  
Bone Marrow ◽  
Ewing Sarcoma ◽  
Core Biopsy ◽  
Biopsy Specimens

Detection of the MPL W515L mutation in bone marrow core biopsy specimens with essential thrombocythemia using the TaqMan assay

2007 ◽  
Vol 38 (10) ◽  
pp. 1581-1582 ◽  
Author(s):  
Camille Laurent ◽  
Véronique Demas ◽  
Eric Delabesse ◽  
Pierre Brousset
Keyword(s):  
Bone Marrow ◽  
Essential Thrombocythemia ◽  
Core Biopsy ◽  
Taqman Assay ◽  
Biopsy Specimens

Bone marrow solid core biopsy needle: a critical assessment of the utility, benefits and limitations of the instruments employed in current day haematology and oncology

2017 ◽  
Vol 71 (6) ◽  
pp. 475-482 ◽  
Author(s):  
Anwarul Islam
Keyword(s):  
Bone Marrow ◽  
Core Biopsy ◽  
Bone Marrow Aspiration ◽  
Histological Evaluation ◽  
Solid Core ◽  
Biopsy Needle ◽  
Morphological Evaluation ◽  
Biopsy Specimens ◽  
Single Use ◽  
User Friendly

The optimal clinical evaluation of the bone marrow requires an examination of air-dried and well-stained films of the aspirated tissue along with a histopathological evaluation of adequately processed and properly stained core biopsy specimens. A bone marrow evaluation can be essential in establishing a diagnosis, determining the efficacy of treatment in haematological disorders and to monitor haematological status of patients following bone marrow/stem cell transplantation. It is also an essential component of the staging process for newly diagnosed malignancies. Currently available bone marrow aspiration needles are quite satisfactory and if properly used provide good-quality specimens for morphological evaluation. However, if a bone marrow core biopsy is concerned, several needles are currently in use but not all of them provide good-quality biopsy specimens for histological evaluation or are user friendly. We have compared the recently introduced Moeller Medical single use bone marrow core biopsy needle with the Jamshidi needle with marrow acquisition cradle (CareFusion), J-needle (Cardinal Health) and OnControl device (Vidacare). It is concluded that the Moeller Medical needle system has definite advantages over others and is recommended for routine use.

scholarly journals An Unsuspected Finding of t(9;22): A Rare Case of Philadelphia Chromosome-Positive B-Lymphoblastic Lymphoma

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Prajwal Boddu ◽  
C. Cameron Yin ◽  
Rashmi Kanagal-Shamanna ◽  
Guillin Tang ◽  
Beenu Thakral ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Kinase Inhibitors ◽  
Bone Marrow Involvement ◽  
Philadelphia Chromosome ◽  
Lymphoblastic Lymphoma ◽  
Accurate Diagnosis ◽  
Biopsy Specimens ◽  
B Lymphoblastic Lymphoma ◽  
Prophylactic Chemotherapy

While rare, cases of isolated extramedullary disease of B-cell Lymphoblastic Lymphoma (B-LBL) without morphologic bone marrow involvement have been described. In this report, we illustrate the case of an elderly gentleman who presented with isolated testicular and vertebral LBL involvement but had no morphologic bone marrow involvement. The initial plan of treatment was to treat along the lines of Philadelphia negative B-ALL/LBL. During this time, fluorescence in situ hybridization (FISH) and PCR testing for BCR-ABL1 rearrangements were being performed on the marrow specimens as a part of routine diagnostic workup. While the FISH returned negative, PCR testing unexpectedly detected BCR-ABL1 fusion transcripts at a low level of 0.48%. Given their presence, we performed FISH for BCR/ABL1 rearrangement in both testicular and L5 vertebral specimens which were 80–90% positive. He subsequently received rituximab, hyper-CVAD, and dasatinib, along with prophylactic intrathecal prophylactic chemotherapy. The patient achieved a prolonged remission but eventually relapsed, 4 years later. Had it not been for this fortuitous discovery, the patient would not have been treated with tyrosine kinase inhibitors. We emphasize that FISH and PCR testing for BCR-ABL1 rearrangement are integral to arriving at an accurate diagnosis and should be routinely tested on B-LBL biopsy specimens.

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