scholarly journals Comparison of counter-current immunoelectrophoresis, latex agglutination, and radioimmunoassay in detection of soluble capsular polysaccharide antigens of Haemophilus influenzae type b and Neisseria meningitidis of groups A or C.

1978 ◽  
Vol 31 (12) ◽  
pp. 1172-1176 ◽  
Author(s):  
M Leinonen ◽  
H Kayhty
Keyword(s):  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Capsular Polysaccharide ◽  
Latex Agglutination ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Polysaccharide Antigens ◽  
Counter Current

scholarly journals Different seroprevalences of antibodies against Neisseria meningitidis serogroup A and Haemophilus influenzae type b in Sudanese and Swedish children

1993 ◽  
Vol 110 (2) ◽  
pp. 307-316 ◽  
Author(s):  
M. A. M. Salih ◽  
H. Fredlund ◽  
S. Hugosson ◽  
L. Bodin ◽  
P. Olcén
Keyword(s):  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Endemic Area ◽  
Capsular Polysaccharide ◽  
Herd Immunity ◽  
Serum Antibody ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Antibody Assay

SUMMARYSampling of sera from 202 Sudanese and 124 Swedish children 1–14 years of age was conducted at the end of the 1980s presenting an opportunity to compare the seroprevalence of anti-Neisseria meningitidis (MC) serogroup A antibodies in an area immediately before outbreak of an epidemic (Sudan 1988) with a low endemic area (Sweden). An ELISA antibody assay was developed for detection of antibodies against capsular polysaccharide of MC serogroup A and Haemophilus influenzae type b (Hib). Serum antibody against MC serogroup A was found significantly more frequently in Sudanese than in Swedish children. This indicates that factors other than herd immunity, as measured by serum antibodies against MC serogroup A polysaccharide, are important for avoidance of an MC serogroup A epidemic. The seroprevalence of Hib antibodies was, in contrast, significantly higher in Swedish than in Sudanese children, especially for 5–9-year-old children. A possible explanation may be the different systems of day-care of children in the two countries.

Advantage of Latex Agglutination Over Countercurrent Immunoelectrophoresis in the Detection of Haemophilus influenzae Type b Antigen in Serum

PEDIATRICS ◽  
1981 ◽  
Vol 68 (6) ◽  
pp. 888-891
Author(s):  
David W. Scheifele ◽  
Joel I. Ward ◽  
George R. Siber
Keyword(s):  
Haemophilus Influenzae ◽  
Latex Particle ◽  
Capsular Polysaccharide ◽  
Latex Agglutination ◽  
Haemophilus Influenzae Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Valuable Adjunct ◽  
Invasive Infections

Detection of bacterial antigens has proved to be a valuable adjunct to early diagnosis of certain invasive infections. In the case of Haemophilus influenzae type b infections, capsular polysaccharide antigen (PRP) has been detected by a variety of techniques including countercurrent immunoelectrophoresis (CIE),1-3 latex particle agglutination (LPA),3,4 radioimmunoassay,5 and enzyme-linked immunosorbent assay (ELISA).6,7 There have been few prospective comparisons3,7,8 by which to establish the sensitivities and idiosyncrasies of these techniques. This report compares the two simplest techniques, CIE and LPA, in children with H influenzae b infections, drawing attention to a shortcoming of CIE in those with nonmeningeal infections. METHODS

Immunogenicity of Haemophilus influenzae Type b Polysaccharide-Neisseria meningitidis Outer Membrane Protein Conjugate Vaccine in 2- to 6-Month-Old Infants

PEDIATRICS ◽  
1987 ◽  
Vol 80 (2) ◽  
pp. 283-287
Author(s):  
Allen A. Lenoir ◽  
Paul D. Granoff ◽  
Dan M. Granoff
Keyword(s):  
Membrane Protein ◽  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Outer Membrane Protein ◽  
Geometric Mean ◽  
Serum Antibody ◽  
Haemophilus Influenzae Type ◽  
Antibody Concentration ◽  
Haemophilus Influenzae Type B ◽  
Type B

Fifty infants, 2 to 6 months of age, were vaccinated with Haemophilus influenzae type b capsular polysaccharide covalently linked to an outer membrane protein from Neisseria meningitidis group B. Subjects were given two injections and were randomly assigned to receive the injections separated by 1 or 2 months. Each dose contained 15 µg of polysaccharide and 51 µg of protein, or approximately twice the amount of polysaccharide as used in our previous trial (Lancet 1986;2:299). Fevers of 38.0° to 38.8°C developed in three infants (6%) within 24 hours after vaccination, but there were no other notable reactions. Following one injection, the geometric mean antibody concentration increased from 0.13 µg/mL in preimmune serum to 1.50 µg/mL in serum obtained 1 to 2 months later (P < .001). After a second injection, there was a further increase in serum antibody (geometric mean = 3.11 µg/mL, P < .007). The geometric mean antibody concentration of the group reimmunized 2 months after the first injection was higher than that in the group reimmunized after 1 month (3.95 v 2.32 µg/mL, P = .05, by analysis of covariance with age as the covariant). These data confirm our previous preliminary observations on the safety and immunogenicity of this new conjugate vaccine in infants 2 to 6 months of age. The data suggest that a 2-month interval between the first and second injections results in higher levels of serum antibody than a 1-month interval.

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