REMOVAL OF HOST ANTIGENS FROM PLANT VIRUS PREPARATIONS BY ION EXCHANGE CHROMATOGRAPHY

1961 ◽  
Vol 39 (7) ◽  
pp. 1705-1709 ◽  
Author(s):  
J. H. Tremaine
Keyword(s):  
Ion Exchange ◽  
Plant Virus ◽  
Analytical Ultracentrifugation ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Plant Viruses ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Serological Tests

The separation of a carnation virus from smaller cowpea-host antigens was achieved by column chromatography on a strongly basic ion-exchange resin. The separation was demonstrated by infectivity and serological tests, analytical ultracentrifugation, and ultraviolet spectrophotometry. The method is proposed for the removal of similar host components from preparations of other spherical plant viruses.

Improved Synthesis and Rapid Isolation of Millimole Quantities of Adenylylsulfate

1979 ◽  
Vol 34 (5-6) ◽  
pp. 346-349 ◽  
Author(s):  
Bryan P. Cooper ◽  
Hans G. Trüper
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Thiobacillus Denitrificans ◽  
Enzymatic Method ◽  
Rapid Isolation ◽  
Aps Reductase

Abstract An improved enzymatic method for the synthesis of adenylylsulfate (APS) from adenosine 5′-phosphate using APS-reductase from Thiobacillus denitrificans is described. Isolation of millimole quantitities of this sulfur nucleotide is achieved rapidly by means of ion exchange chromatography on a strongly basic ion exchange resin. A facile and reproducible desalting procedure is described.

Fossil mollusk shell organic matrix components preserved for 80 million years

Paleobiology ◽  
1979 ◽  
Vol 5 (2) ◽  
pp. 144-150 ◽  
Author(s):  
S. Weiner ◽  
H. A. Lowenstam ◽  
B. Taborek ◽  
L. Hood
Keyword(s):  
Ion Exchange ◽  
Upper Cretaceous ◽  
Exchange Resin ◽  
Organic Matrix ◽  
Ion Exchange Resin ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Diagenetic Alteration ◽  
Matrix Components ◽  
Shell Organic Matrix

The organic matrix components of a fossil ammonoid shell from the Upper Cretaceous, can be separated into sub-fractions which are generally comparable to those found in extant Nautilus, using ion exchange chromatography. This suggests that at least portions of these components are sufficiently well preserved to interact characteristically with the ion exchange resin. Amino acid compositions of these sub-fractions, however, do not resemble Nautilus organic matrix sub-fractions, indicating that considerable diagenetic alteration of this material has taken place.

scholarly journals A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery

2009 ◽  
Vol 52 (2) ◽  
pp. 427-436 ◽  
Author(s):  
João Batista Severo Jr. ◽  
Roberto Rodrigues de Souza ◽  
José Carlos Curvelo Santana ◽  
Elias Basile Tambourgi
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Binding Capacity ◽  
Ion Exchange Resin ◽  
Exchange Chromatography ◽  
Expanded Bed Adsorption ◽  
Expanded Bed ◽  
Bed Height ◽  
Salt Addition ◽  
Bed Expansion

In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.

False-negative results for urinary phenothiazines and imipramine in Forrest's qualitative assays.

1980 ◽  
Vol 26 (2) ◽  
pp. 345-347 ◽  
Author(s):  
G P James ◽  
M H DJang ◽  
H H Hamilton
Keyword(s):  
Ascorbic Acid ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
False Negative ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Negative Results ◽  
False Negative Results

Abstract When a series of patients' urine samples supplemented in vitro with chlorpromazine or imipramine was assayed with the Forrest qualitative assays, we observed an occasional false-negative result, which we found was attributable to interference by ascorbic acid. It interferes with the reagent, not with the analytes, in both assays. We easily eliminated this interference with the phenothiazine test by using an anion-exchange resin. Eliminating the interference with the assay for imipramine, however, is more difficult; false-negative results can be obtained even after ion-exchange chromatography if the imipramine concentration is less than 50 mg/L.

Separation and quantification of 90Sr from ion-exchange resin radioactive waste: methods and techniques of analysis

2020 ◽  
Vol 108 (8) ◽  
pp. 627-640
Author(s):  
Aurelia Magdalena Dianu ◽  
Relu Ion Dobrin
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Extraction Process ◽  
Ion Exchange Resin ◽  
Exchange Chromatography ◽  
Chemical Recovery ◽  
Convincing Argument ◽  
Sample Decomposition ◽  
Pre Treatment ◽  
Chromatographic Extraction

AbstractFour methods for 90Sr separation from spent ion-exchange resin samples were carried out, offering a useful methodology to achieve interferences free 90Sr fractions. The four methods consist in resin sample decomposition, pre-treatment and selective separation of 90Sr by using: (a) a single chromatographic extraction process, (b) double chromatographic extraction, (c) a single chromatographic extraction process followed in sequence by two precipitations, and (d) ion-exchange chromatography, followed by extraction chromatography and precipitation. Mineralization by microwave acid digestion and the four 90Sr separation methods thoroughly presented are available. Data processing methods (adjustable modified efficiency tracing – a new improved approach for the efficiency tracing LSC technique, non-linear regression and α-β discrimination) to obtain the activities values of α, β-γ, pure β emitters and the evaluation of chemical recovery yield of strontium were presented. A discussion about activity assessment in 90Sr purified fractions, providing a convincing argument to support the accuracy of the 90Sr separation methods, is also offered.

False-negative results for urinary phenothiazines and imipramine in Forrest's qualitative assays.

1980 ◽  
Vol 26 (2) ◽  
pp. 345-347
Author(s):  
G P James ◽  
M H DJang ◽  
H H Hamilton
Keyword(s):  
Ascorbic Acid ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
False Negative ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Negative Results ◽  
False Negative Results

Abstract When a series of patients' urine samples supplemented in vitro with chlorpromazine or imipramine was assayed with the Forrest qualitative assays, we observed an occasional false-negative result, which we found was attributable to interference by ascorbic acid. It interferes with the reagent, not with the analytes, in both assays. We easily eliminated this interference with the phenothiazine test by using an anion-exchange resin. Eliminating the interference with the assay for imipramine, however, is more difficult; false-negative results can be obtained even after ion-exchange chromatography if the imipramine concentration is less than 50 mg/L.

Ion Exchange Method for Determining Mephentermine Sulfate in Drug Formulations: Collaborative Study

1978 ◽  
Vol 61 (1) ◽  
pp. 60-64
Author(s):  
Rod Chu ◽  
Elaine J Friedman
Keyword(s):  
Ion Exchange ◽  
Collaborative Study ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Drug Formulations ◽  
Ultraviolet Spectrophotometry ◽  
Exchange Method ◽  
Coefficients Of Variation ◽  
Ion Exchange Method

Abstract Ion exchange chromatography is used to separate mephentermine sulfate from drug formulations. The drug is subsequently measured by ultraviolet spectrophotometry. Assays on 4 commercial samples of tablets and injectables gave recoveries from 97.6 to 104% of declared. A collaborative study involving 2 tablet and 2 liquid formulations gave mean recoveries ranging from 94.7 to 99.3% and coefficients of variation from 1.39 to 2.00%.

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