A simple technique for making epidermal imprints using clear vinyl film

1985 ◽  
Vol 63 (9) ◽  
pp. 1669-1670 ◽  
Author(s):  
R. E. Redmann
Keyword(s):  
Microscopic Study ◽  
Polyvinyl Chloride ◽  
Simple Technique ◽  
Leaf Surface ◽  
Grass Species ◽  
Simple Method ◽  
Corrugated Surfaces ◽  
Chloride Film

Transparent, plasticized polyvinyl chloride film, pressed against a leaf surface and heated, produced an epidermal impression suitable for microscopic study. This rapid, simple method was tested successfully on 39 grass species, including those with corrugated surfaces and trichomes.

Precision cutting of polyvinyl chloride film by ultraviolet nanosecond laser

2021 ◽  
pp. 1-8
Author(s):  
Congyi Wu ◽  
Youmin Rong ◽  
Yu Huang ◽  
Min Li ◽  
Guojun Zhang ◽  
...  
Keyword(s):  
Polyvinyl Chloride ◽  
Nanosecond Laser ◽  
Chloride Film

A (Relatively) Simple Method to Visualize Single Molecules

2011 ◽  
Vol 19 (1) ◽  
pp. 8-8
Author(s):  
Stephen W. Carmichael ◽  
Julio M. Fernandez
Keyword(s):  
Fluorescence Microscopy ◽  
Simple Technique ◽  
Zero Mode ◽  
Single Molecules ◽  
Simple Method ◽  
Expensive Equipment ◽  
Freely Moving ◽  
Good For

Fluorescence microscopy can be used to study certain single molecules in solution or attached to a surface. Two conflicting challenges to overcome are: (1) to image freely moving molecules for long times and (2) to image immobilized single molecules when there is a highly fluorescent background. The fact that these two goals are inversely related is illustrated by epifluorescence, which is good for observing freely diffusing molecules but poor for detecting single molecules, whereas the reverse is true for zero-mode waveguides. Plus, these and other techniques require elaborate (read: expensive) equipment with computerized controls. Sabrina Leslie, Alexander Fields, and Adam Cohen have developed an ingenious (relatively) simple technique that can image freely moving single molecules.

scholarly journals A simple method to determine leaf angles of grass species

2000 ◽  
Vol 51 (349) ◽  
pp. 1467-1470 ◽  
Author(s):  
G. Deckmyn ◽  
I. Nijs ◽  
R. Ceulemans
Keyword(s):  
Grass Species ◽  
Simple Method

Characterisation of glyphosate uptake into grass species

2003 ◽  
Vol 54 (9) ◽  
pp. 877 ◽  
Author(s):  
Z. Q. Liu
Keyword(s):  
Leaf Surface ◽  
Current Knowledge ◽  
Grass Species ◽  
Droplet Spreading ◽  
Barnyard Grass ◽  
Spread Area ◽  
Per Se ◽  
High Level ◽  
Content Range ◽  
Determining Factor

The effect of surfactant ethylene oxide (EO) content, droplet spread area, and the active ingredient (a.i.) dose on the leaf surface on glyphosate uptake into 3 grass species (wheat, ryegrass, and barnyard grass) was investigated. It was found that glyphosate uptake into wheat was only moderately correlated (R2 = 0.66) with surfactant EO content (range 5–20 units per molecule). By contrast, a highly negative correlation (R2 = 0.98) was found between the uptake and the spread area of the treatment formulations on the leaf surface. The influence of droplet spreading on glyphosate uptake was further investigated using a super-spreading surfactant, Silwet L-77, and varying a.i. concentrations. It was demonstrated that the determining factor for glyphosate uptake into all 3 grass species was the a.i. dose (in μg/mm2) formed on the leaf surface after droplet spreading. Droplet spread area per se had only an indirect effect on the uptake through diluting the a.i. dose. In the presence of a non-spreading surfactant, Mon 0818, glyphosate uptake depended not only on the a.i. dose but also the surfactant dose on the leaf surface. In the absence of surfactants, high a.i. dose alone was not enough to ensure a high level uptake. The results are discussed in relation to the current knowledge on the interactions between surfactants and herbicide uptake.

A SIMPLE METHOD FOR THE ASSAY OF SMALL AMOUNTS OF ANTIDIURETIC HORMONE

1959 ◽  
Vol 37 (10) ◽  
pp. 1215-1225 ◽  
Author(s):  
J. Hunter ◽  
H. Kalant ◽  
J. C. Ogilvie
Keyword(s):  
Antidiuretic Hormone ◽  
Simple Technique ◽  
Nacl Solution ◽  
Albino Rat ◽  
Simple Method ◽  
Water Load ◽  
True Value ◽  
Statistical Precision ◽  
Urine Production ◽  
Animal Material

A reliable, sensitive, and simple technique is described for the assay of antidiuretic hormone in amounts between 25 and 130 μunits. The assay animal is a conscious albino rat weighing between 150 and 250 g. On the day of assay it is tranquillized with an injection of perphenazine. Specially designed cannulae, implanted on the day before the assay, provide unimpaired access to the stomach and bladder, permitting the maintenance of hydration and continuous urine collection without disturbance of the animal. Material to be assayed is injected intravenously. Water load is maintained by manual injection of 0.5-ml quantities of 0.05% NaCl solution at intervals dependent upon the rate of urine production. A useful index for expression of the response is described, which is related to both the intensity and the duration of antidiuresis. Assay of an artificial unknown by this method gave a result within 6% of the true value, and an excellent statistical precision (λ = 0.143).

A SIMPLE METHOD FOR THE ASSAY OF SMALL AMOUNTS OF ANTIDIURETIC HORMONE

1959 ◽  
Vol 37 (1) ◽  
pp. 1215-1225
Author(s):  
J. Hunter ◽  
H. Kalant ◽  
J. C. Ogilvie
Keyword(s):  
Antidiuretic Hormone ◽  
Simple Technique ◽  
Nacl Solution ◽  
Albino Rat ◽  
Simple Method ◽  
Water Load ◽  
True Value ◽  
Statistical Precision ◽  
Urine Production ◽  
Animal Material

A reliable, sensitive, and simple technique is described for the assay of antidiuretic hormone in amounts between 25 and 130 μunits. The assay animal is a conscious albino rat weighing between 150 and 250 g. On the day of assay it is tranquillized with an injection of perphenazine. Specially designed cannulae, implanted on the day before the assay, provide unimpaired access to the stomach and bladder, permitting the maintenance of hydration and continuous urine collection without disturbance of the animal. Material to be assayed is injected intravenously. Water load is maintained by manual injection of 0.5-ml quantities of 0.05% NaCl solution at intervals dependent upon the rate of urine production. A useful index for expression of the response is described, which is related to both the intensity and the duration of antidiuresis. Assay of an artificial unknown by this method gave a result within 6% of the true value, and an excellent statistical precision (λ = 0.143).

Plasticized polyvinyl chloride film as a primary burns dressing: a microbiological study

Burns ◽  
1988 ◽  
Vol 14 (1) ◽  
pp. 62-65 ◽  
Author(s):  
R.H. Milner ◽  
S.J. Hudson ◽  
C.A. Reid
Keyword(s):  
Polyvinyl Chloride ◽  
Microbiological Study ◽  
Chloride Film
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