Cytoembryology of Amaryllis hybrids

1989 ◽  
Vol 67 (3) ◽  
pp. 839-847 ◽  
Author(s):  
Tasneem F. Khaleel ◽  
Daniel Siemsen
Keyword(s):  
Chromosome Number ◽  
Seed Set ◽  
Karyotype Analysis ◽  
Pollen Grains ◽  
Basic Chromosome Number ◽  
Archesporial Cell ◽  
Anther Wall ◽  
Basic Chromosome ◽  
Development Of Gametophytes ◽  
Secretory Type

Karyotype analysis, sporogenesis, and the development of gametophytes are described for four hybrids of garden Amaryllis. The basic chromosome number is x = 11, and all four hybrids are tetraploid. The basikaryotype, which consists of two median, five submedian, and four subterminal chromosomes, is traceable in four hybrids. The anther wall is five to six layered. The tapetum is of the secretory type, and its cells become two nucleate at the onset of meiosis in the microsporocytes. Bivalents, trivalents, and quadrivalents are formed during meiosis. The endothecial cells lack fibrillar thickenings. Pollen grains are shed at the two-celled stage. About 60–70% of the pollen grains are fertile. Ovules are anatropous and bitegmic. The archesporial cell may or may not undergo periclinal division. Development of the megagametophyte is monosporic in 90% of the ovules and bisporic in the remaining 10%. All four hybrids show degeneration of embryo sacs at various stages of development which accounts for the low percentage or lack of seed set.

CYANIXIA, A NEW GENUS FOR THE SOCOTRAN ENDEMIC BABIANA SOCOTRANA (IRIDACEAE–CROCOIDEAE)

2003 ◽  
Vol 60 (3) ◽  
pp. 517-532 ◽  
Author(s):  
P. GOLDBLATT ◽  
J. C. MANNING ◽  
J. DAVIES ◽  
V. SAVOLAINEN ◽  
S. REZAI
Keyword(s):  
Chromosome Number ◽  
New Genus ◽  
Pollen Grains ◽  
Basic Chromosome Number ◽  
Sub Saharan Africa ◽  
Chloroplast Gene ◽  
Basic Chromosome ◽  
Sub Saharan ◽  
Common Situation ◽  
African Genus

The sub-Saharan and mainly western southern African genus Babiana (Iridaceae–Crocoideae) is morphologically diverse, containing some 80 species. The Socotran B. socotrana, which differs from all other members of the genus inter alia in its trisulcate pollen grains, basic chromosome number, x=10, corms of axillary origin, and globose, colliculate–tuberculate seeds, is here excluded from Babiana and referred to the new genus Cyanixia. Other Babiana species have monosulcate pollen grains with a two-banded operculum, a basic chromosome number of x=7, terminal corm ontogeny, and derived pear-shaped seeds with a smooth glossy surface. Cyanixia socotrana was previously assigned to Babiana because of superficial similarities in morphology, including plicate leaves and blue flowers, thought at the time to be bilabiate, although now known to be actinomorphic and rotate. DNA sequence analysis using the chloroplast gene matK confirms that Babiana is a monophyletic assemblage, whereas B. socotrana is sister to the Lapeirousia/Savannosiphon clade of sub-Saharan Africa. The latter shares axillary corm ontogeny with the southern African Micranthus, Thereianthus, and Watsonia, in contrast to the more common situation in Crocoideae (including Babiana) in which the corm develops from the base of the flowering stem. The tropical African Zygotritonia, which also has axillary corm development, shares with Cyanixia a trisulcate pollen grain, but it differs markedly from that genus in its small, zygomorphic flower, undivided style, and basic chromosome number x=8. Cyanixia is here described as a new monotypic genus of Crocoideae, probably most closely allied to Savannosiphon, also a monospecific genus.

Karyotype analysis of medicinal plant Liriope spicata var. prolifera (Liliaceae)

Biologia ◽  
2009 ◽  
Vol 64 (4) ◽  
Author(s):  
Qun Zhou ◽  
Jianqiu Zhou ◽  
Jiachun Chen ◽  
Xiaogang Wang
Keyword(s):  
Chromosome Number ◽  
Medicinal Plant ◽  
Endemic Species ◽  
Secondary Constriction ◽  
Karyotype Analysis ◽  
Basic Chromosome Number ◽  
Separate Species ◽  
Basic Chromosome ◽  
First Time

AbstractKaryotype of Liriope spicata var. prolifera, a Chinese endemic species, was described in detail for the first time. Its proto-variety L. spicata was also investigated for comparison. The basic chromosome number of these two species was x = 18. L. spicata var. prolifera, recorded as triploid 2n = 54, consisted of 30 metacentric chromosomes and 24 submetacentric chromosomes. Only one chromosome of the 11th group had a secondary constriction with a satellite in the short arm. L. Spicata was tetraploid 2n = 72 and consisted of four sets of 6 submetacentric chromosomes and 12 metacentric chromosomes without visible satellites. This paper provides further available data on Liriope chromosomes, and also indicates that L. spicata var. prolifera and L. spicata are probably separate species.

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals Relations and evolution in Cheilanthes (Sinopteridaceae, Pteridophita) in Macaronesia and Mediterranean area, deduced from genome analysis of their hybrids

1983 ◽  
Vol 8 ◽  
pp. 101-126 ◽  
Author(s):  
G. Vida ◽  
A. Major ◽  
T. Reichstein
Keyword(s):  
Chromosome Number ◽  
Genome Analysis ◽  
Chromosome Doubling ◽  
Diploid Species ◽  
Mediterranean Area ◽  
Basic Chromosome Number ◽  
The Other ◽  
Experimental Conditions ◽  
Natural Group ◽  
Basic Chromosome

Nine species of "Cheilantoid ferns" are known to grow in Macaronesia and the Mediterranean basin. Two of them (lacking a pseudo-indusium and having the basic chromosome number X = 29), both aggregate species which we prefer to retain in Notholaena, are not included in this study. The other seven species (with distinct pseudo-indusium and the basic chromosome number X = 30), which we accept as members of the genus Cheilanthes Sw. sensu stricto, were subjected to detailed genome analysis of their natural and experimentally produced hybrids and shown to represent an aggregate of four very distinct ancestral diploids and three allotetraploids. The latter must have once been formed by chromosome doubling in the three diploid hybrids of C. maderensis Lowe with the other three diploid species. Theoretically three more allotetraploids would be possible but their formation has obviously been prevented by the geographical separation of the three respective diploids. The most widely distributed of the tetraploids, i.e. C. pteridioides (Reich.) C.Chr. has also been resynthesized from its ancestors (still sympatric) under experimental conditions. The intermediate morphology of the allotetraploids (as compared with their diploid ancestors) is obviously the reason why their status and existence has so long escaped recognition in Europe. These seven species form a natural group and, in our opinion, should not be divided into sections.

Meiotic chromosome behaviour in Cenchrus ciliaris

Bothalia ◽  
1998 ◽  
Vol 28 (1) ◽  
pp. 83-90 ◽  
Author(s):  
N. C. Visser ◽  
J. J. Spies
Keyword(s):  
Chromosome Number ◽  
Meiotic Chromosome ◽  
Basic Chromosome Number ◽  
Chromosome Behaviour ◽  
Meiotic Abnormalities ◽  
Single Specimen ◽  
Cenchrus Ciliaris ◽  
Basic Chromosome ◽  
Single Genome ◽  
Meiotic Irregularities

A basic chromosome number of x = 9 has been confirmed for Cenchrus ciliaris L. Polyploidy is common and levels vary from tetraploid to hexaploid. Aneuploidv is reported for a single specimen, where two chromosomes of a single genome were lost. Various meiotic irregularities were observed. The highest incidence of meiotic abnormalities was observed in the pentaploid specimens. This was attributed to their uneven polyploid level All specimens varied from segmental alloploid to alloploid.

Chromosome studies on African plants. 9. Chromosome numbers in Ehrharta (Poaceae: Ehrharteae)

Bothalia ◽  
1989 ◽  
Vol 19 (1) ◽  
pp. 125-132 ◽  
Author(s):  
J. J. Spies ◽  
E. J. L. Saayman ◽  
S. P. Voges ◽  
G. Davidse
Keyword(s):  
Chromosome Number ◽  
Ploidy Level ◽  
Chromosome Numbers ◽  
Basic Chromosome Number ◽  
B Chromosomes ◽  
Basic Chromosome ◽  
Cytogenetic Studies ◽  
African Plants ◽  
First Time

Cytogenetic studies of 53 specimens of 14 species of the genus  Ehrharta Thunb. confirmed a basic chromosome number of 12 for the genus. Chromosome numbers for 13 species are described for the first time. The highest ploidy level yet observed in the genus (2n = lOx = 120) is reported for E. villosa var.  villosa. B chromosomes were observed in several specimens of four different species.

Chromosome studies on African plants. 11. The tribe Andropogoneae (Poaceae: Panicoideae)

Bothalia ◽  
1994 ◽  
Vol 24 (2) ◽  
pp. 241-246 ◽  
Author(s):  
J. J. Spies ◽  
T. H. Troskie ◽  
E. Van der Vyver ◽  
S. M. C Van Wyk
Keyword(s):  
Chromosome Number ◽  
Basic Chromosome Number ◽  
Basic Chromosome ◽  
African Plants

Representative specimens of various species of the genera  Andropogon L.,  Cymbopogon Spreng.,  Elionurus Kunth ex Willd.,  Hyparrhenia Foum. and  Hyperthelia Clayton were cytogenetically studied. All specimens had a secondary basic chromosome number of ten. Polyploidy, either as alloploidy or segmental alloploidy. was frequent. The taxa studied represent mature polyploid complexes.  

Infraspecific polyploidy and gynodioecism in Ptilotus obovatus (Amaranthaceae)

1976 ◽  
Vol 24 (2) ◽  
pp. 237 ◽  
Author(s):  
D.A. Stewart ◽  
BA Barlow
Keyword(s):  
Chromosome Number ◽  
Sex Ratio ◽  
Dynamic Equilibrium ◽  
Arid Zone ◽  
Basic Number ◽  
Basic Chromosome Number ◽  
Genetic Effects ◽  
Basic Chromosome ◽  
Species Area ◽  
Selective Effects

A basic chromosome number of x = 27 is constant in 14 species of Ptilotus examined. This basic number may be polyploid in derivation, with the entire genus having developed at a stabilized hexaploid level from an ancestral stock with x = 9. Only one species, P. obovatus (Gaud.) F. Muell., shows cytotypic variation, with diploid and tetraploid forms having n = 27 and n = 54 respectively. The tetraploid biotype is relatively uniform morphologically and is distributed throughout the species area studied. Diploid biotypes are more variable morphologically and of more local occurrence, and may be isolated relicts. The adaptive tetraploid biotype has probably been a more successful recolonizer of the arid zone following Recent arid maxima. Gynodioecism is of general occurrence in both diploid and tetraploid races of P. obovatus, and is probably effective as an outcrossing mechanism in this self-compatible species. The sex ratio varies between populations, and may be in dynamic equilibrium with the genetic effects of polyploidy and the selective effects of habitat stability.

Sign in / Sign up

Export Citation Format

Share Document