Organic solvent-tolerant mutants of Pseudomonas aeruginosa display multiple antibiotic resistance

1999 ◽  
Vol 45 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Xian-Zhi Li ◽  
Keith Poole
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Organic Solvent ◽  
Multiple Antibiotic Resistance ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

A Novel Strain Pseudomonas Aeruginosa CS-2 Producing an Organic Solvent-Tolerant Lipase

2011 ◽  
Vol 343-344 ◽  
pp. 774-780
Author(s):  
Ren Peng ◽  
Jin Ping Lin ◽  
Dong Zhi Wei
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Organic Solvent ◽  
Gum Arabic ◽  
Lipase Production ◽  
16S Rdna Sequence ◽  
Organic Solvent Tolerant ◽  
Initial Ph ◽  
Improved Stability ◽  
Solvent Tolerant ◽  
Physiological And Biochemical

Organic solvent-tolerant lipases have shown new potential application in biotechnological fields. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China. Based on the morphological, physiological and biochemical features and 16S rDNA sequence of the strain, it was identified asPseudomonas aeruginosaCS-2. The crude lipase had an improved stability in the presence of acetonitrile and it exhibited stability in presence of benzene, chloroform,n-Hexane, petroleum ether and isooctane. The optimized composition of culture medium was as follows: olive oil 1%, peptone 4%, K2HPO40.5%, MgSO4·7H2O 0.1%, gum arabic 0.04%. The optimum condition for lipase production was on the following: temperature 37°C, initial pH 7.5, rotary shaking speed 200 rev/min, culture time 48 h.

Unravelling protein -organic solvent interaction of organic solvent tolerant elastase from Pseudomonas aeruginosa strain K crystal structure

2019 ◽  
Vol 127 ◽  
pp. 575-584 ◽  
Author(s):  
Zatty Syamimi @. Adura Mat Said ◽  
Fatin Amirah Mohd Arifi ◽  
Abu Bakar Salleh ◽  
Raja Noor Zaliha Raja Abd Rahman ◽  
Adam Thean Chor Leow ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Pseudomonas Aeruginosa ◽  
Organic Solvent ◽  
Solvent Interaction ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

Lipase production in two-step fed-batch culture of organic solvent-tolerant Pseudomonas aeruginosa LST-03

2001 ◽  
Vol 91 (3) ◽  
pp. 245-250 ◽  
Author(s):  
Takeshi Ito ◽  
Hirokazu Kikuta ◽  
Eiji Nagamori ◽  
Hiroyuki Honda ◽  
Hiroyasu Ogino ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Organic Solvent ◽  
Batch Culture ◽  
Lipase Production ◽  
Fed Batch ◽  
Organic Solvent Tolerant ◽  
Fed Batch Culture ◽  
Solvent Tolerant

Organic solvent-tolerant mutants of Pseudomonas aeruginosa display multiple antibiotic resistance

1999 ◽  
Vol 45 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Xian-Zhi Li ◽  
Keith Poole
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Organic Solvent ◽  
Antimicrobial Agents ◽  
Efflux Pump ◽  
Environmental Pollutants ◽  
Multidrug Resistant ◽  
Solvent Tolerance ◽  
Organic Solvent Tolerance ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant

Organic solvent-tolerant mutants of Pseudomonas aeruginosa selected in the presence of hexane exhibited increased resistance to a variety of structurally unrelated antimicrobial agents, including beta-lactams, fluoroquinolones, chloramphenicol, tetracycline, and novobiocin, a phenotype typical of nalB multidrug-resistant mutants. Western immunoblotting with antibodies specific to components of the three known multidrug efflux systems in P. aeruginosa demonstrated that the solvent-tolerant mutants displayed increased expression of the MexAB-OprM system and decreased expression of the MexEF-OprN system. Sequence analysis of mexR, the repressor gene of mexAB-oprM efflux operon, identified a nonsense mutation and a point mutation in the mexR genes of two solvent-tolerant mutants. These results emphasize the importance of the MexAB-OprM efflux system in organic solvent tolerance and the ability of environmental pollutants to select bacteria with a medically relevant antibiotic-resistant phenotype.Key words: Pseudomonas aeruginosa, organic solvent tolerance, multidrug resistance, MexAB-OprM efflux pump, mexR gene.

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