Enhanced lipase production from organic solvent tolerant Pseudomonas aeruginosa UKHL1 and its application in oily waste-water treatment

Organic solvent-tolerant lipases have shown new potential application in biotechnological fields. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China. Based on the morphological, physiological and biochemical features and 16S rDNA sequence of the strain, it was identified asPseudomonas aeruginosaCS-2. The crude lipase had an improved stability in the presence of acetonitrile and it exhibited stability in presence of benzene, chloroform,n-Hexane, petroleum ether and isooctane. The optimized composition of culture medium was as follows: olive oil 1%, peptone 4%, K2HPO40.5%, MgSO4·7H2O 0.1%, gum arabic 0.04%. The optimum condition for lipase production was on the following: temperature 37°C, initial pH 7.5, rotary shaking speed 200 rev/min, culture time 48 h.

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Lipase production in two-step fed-batch culture of organic solvent-tolerant Pseudomonas aeruginosa LST-03

Journal of Bioscience and Bioengineering ◽

10.1016/s1389-1723(01)80128-6 ◽

2001 ◽

Vol 91 (3) ◽

pp. 245-250 ◽

Cited By ~ 22

Author(s):

Takeshi Ito ◽

Hirokazu Kikuta ◽

Eiji Nagamori ◽

Hiroyuki Honda ◽

Hiroyasu Ogino ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Organic Solvent ◽

Batch Culture ◽

Lipase Production ◽

Fed Batch ◽

Organic Solvent Tolerant ◽

Fed Batch Culture ◽

Solvent Tolerant

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Lipase Production in Two-Step Fed-Batch Culture of Organic Solvent-Tolerant Pseudomonas aeruginosa LST-03.

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.91.245 ◽

2001 ◽

Vol 91 (3) ◽

pp. 245-250 ◽

Cited By ~ 7

Author(s):

TAKESHI ITO ◽

HIROKAZU KIKUTA ◽

EIJI NAGAMORI ◽

HIROYUKI HONDA ◽

HIROYASU OGINO ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Organic Solvent ◽

Batch Culture ◽

Lipase Production ◽

Fed Batch ◽

Organic Solvent Tolerant ◽

Fed Batch Culture ◽

Solvent Tolerant

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Microbiological quality of residues from drinking water preparation

Water Science & Technology ◽

10.2166/wst.1995.0565 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 75-79 ◽

Cited By ~ 5

Author(s):

M. Würzer ◽

A. Wiedenmann ◽

K. Botzenhart

Keyword(s):

Waste Water ◽

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Drinking Water ◽

Water Treatment ◽

Pathogenic Bacteria ◽

Waste Water Treatment ◽

Microbiological Quality ◽

Detection Limits

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.

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