Isolation and Characterization of an Anaerobic Bacterium, Eubacterium tarantellus sp.nov., Associated with Striped Mullet (Mugil cephalus) Mortality in Biscayne Bay, Florida

1977 ◽  
Vol 34 (3) ◽  
pp. 402-409 ◽  
Author(s):  
Lanny R. Udey ◽  
Elaine Young ◽  
Bennett Sallman
Keyword(s):  
Ictalurus Punctatus ◽  
Anaerobic Bacterium ◽  
Biochemical Characterization ◽  
Anaerobic Bacteria ◽  
Mugil Cephalus ◽  
Estuarine Fish ◽  
Biscayne Bay ◽  
Striped Mullet ◽  
Isolation And Characterization

An asporogenous, gram-positive, anaerobic bacterium was isolated in pure culture from the brains of numerous dead and moribund striped mullet (Mugil cephalus) from Biscayne Bay, Florida. Biochemical characterization confirmed that it was a new species and has been named Eubacterium tarantellus (ATCC 29255). All isolates produced lecithinase and deoxyribonuclease, were beta hemolytic, but only weakly fermented selected carbohydrates. This anaerobe was pathogenic for channel catfish (Ictalurus punctatus) but not for white mice or guinea pigs.More recently E. tarantellus has been isolated from 10 additional fish species. This and other anaerobic bacteria may be significant pathogens of estuarine fish. Key words: Anaerobic bacteria, Eubacterium, Mugil cephalus, and fish diseases.

scholarly journals A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens

2020 ◽  
Vol 40 (7) ◽  
Author(s):  
Feifei Wang ◽  
Yifeng Wei ◽  
Qiang Lu ◽  
Ee Lui Ang ◽  
Huimin Zhao ◽  
...  
Keyword(s):  
Anaerobic Bacterium ◽  
Methyl Viologen ◽  
Flavin Adenine Dinucleotide ◽  
Biochemical Characterization ◽  
Dihydropyrimidine Dehydrogenase ◽  
Anaerobic Bacteria ◽  
Gene Clusters ◽  
Flavin Mononucleotide ◽  
Pyrimidine Degradation

Abstract Dihydropyrimidine dehydrogenase (PydA) catalyzes the first step of the reductive pyrimidine degradation (Pyd) pathway in bacteria and eukaryotes, enabling pyrimidines to be utilized as substrates for growth. PydA homologs studied to date catalyze the reduction of uracil to dihydrouracil, coupled to the oxidation of NAD(P)H. Uracil reduction occurs at a flavin mononucleotide (FMN) site, and NAD(P)H oxidation occurs at a flavin adenine dinucleotide (FAD) site, with two ferredoxin domains thought to mediate inter-site electron transfer. Here, we report the biochemical characterization of a Clostridial PydA homolog (PydAc) from a Pyd gene cluster in the strict anaerobic bacterium Clostridium chromiireducens. PydAc lacks the FAD domain, and instead is able to catalyze uracil reduction using reduced methyl viologen or reduced ferredoxin as the electron source. Homologs of PydAc are present in Pyd gene clusters in many strict anaerobic bacteria, which use reduced ferredoxin as an intermediate in their energy metabolism.

scholarly journals Isolation and characterization of an activator protein for the hydrolysis of ganglioside GM2 from the roe of striped mullet (Mugil cephalus)

1989 ◽  
Vol 260 (3) ◽  
pp. 777-783 ◽  
Author(s):  
R DeGasperi ◽  
Y T Li ◽  
S C Li
Keyword(s):  
Mugil Cephalus ◽  
Striped Mullet ◽  
Activator Protein ◽  
Isolation And Characterization ◽  
Mammalian Tissues ◽  
Hexosaminidase A ◽  
Ammonium Sulphate Fractionation ◽  
Gm2 Activator ◽  
Hydrolysis Of

After the revelation of the presence of ganglioside GM2 as the major ganglioside in the roe of striped mullet, Mugil cephalus [Li, Hirabayashi, DeGasperi, Yu, Ariga, Koerner & Li (1984) J. Biol. Chem. 259, 8980-8985], we have continued to investigate the catabolism of GM2 in this tissue. We have found that mullet roe contains a specific activator protein which stimulates the hydrolysis of GM2 carried out by the beta-hexosaminidase isolated from the same tissue. This activator has been purified by using conventional procedures including ammonium sulphate fractionation and chromatography on Sepharose 6B, DEAE-Sephadex A-50, octyl-Sepharose and Matrex Gel Blue A columns. This activator protein is also able to stimulate the hydrolysis of GM2 carried out by human beta-hexosaminidase A. Unlike human GM2-activator, the roe activator protein does not stimulate the hydrolysis of GgOse3Cer or GbOse4Cer. The molecular mass (18 kDa) of the roe activator protein was found to be similar to that of human GM2-activator; however, the pI (pH 4.1) was found to be lower than that of human GM2-activator. This is the first report on the presence of a GM2-activator protein in a source other than mammalian tissues.

Isolation and characterization of microsatellite loci in the striped mullet, Mugil cephalus

2005 ◽  
Vol 5 (2) ◽  
pp. 323-326 ◽  
Author(s):  
E. MIGGIANO ◽  
R. E. LYONS ◽  
Y. LI ◽  
L. M. DIERENS ◽  
D. CROSETTI ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Mugil Cephalus ◽  
Striped Mullet ◽  
Isolation And Characterization

Isolation and characterization of an extremely thermophilic, cellulolytic, anaerobic bacterium

1988 ◽  
Vol 29 (5) ◽  
pp. 474-479 ◽  
Author(s):  
Masahito Taya ◽  
Haruyuki Hinoki ◽  
Toshiyuki Yagi ◽  
Takeshi Kobayashi
Keyword(s):  
Anaerobic Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Xenobiotic Pesticide Degrading Bacterial Species in Flower Farms Around Lake Naivasha, Kenya

2018 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Biochemical Characterization ◽  
Bacterial Species ◽  
Rhodococcus Erythropolis ◽  
Soil Samples ◽  
Lake Naivasha ◽  
Organophosphate Pesticide ◽  
Lower Growth ◽  
Isolation And Characterization ◽  
Cell Counts

Certain microorganisms especially bacteria and fungi are able to use xenobiotic organic compounds as their carbon and nitrogen source for metabolism. Flower farms around lake Naivasha basin uses several agrochemicals especially pesticides to control pests and improve flower production. The aim of this study was to isolate and characterize morphologically and biochemically the main bacterial species that are able to grow and tolerate the pesticide contaminated farm soils. Soil samples were collected from randomly selected five greenhouses from each five flower farms namely Crescent, Elsamere, Karuturi, Malewa and Sewage farms around Lake Naivasha basin. The collected samples were processed for bacterial isolation using the nutrient agar, mac’ Conkey agar, blood agar, Luria-Bertani and Minimum Salt Media nutrient media. The conventional methods of swabbing and streaking were used. Pure colonies of isolates organisms were identified and characterized using standard microbiological technique. Morphological, cultural and biochemical characterization of bacterial species isolated from the flower farm soil samples identified mainly Pseudomonas auriginosa, Escherichia coli, Rhodococcus erythropolis and Bacillus subtilis species. Bacterial growth in pesticide consortia was quantified by monitoring colony growth of the species in liquid culture over time. The viable cell counts were determined turbidimetrically at O.D696nm. All the isolated bacterial species were able to grow in flower farm soil contaminated with organochloride and organophosphate pesticide residues. B. subtilis recorded the highest growth at 1.77±0.07 O.D696nm in pesticide mixture consortia. There was lower growth in organochloride pesticide consortia as compared to organophosphate pesticide consortia.

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