In vitro Cultivation of Contracaecum osculatum (Nematoda:Anisakidae) from Third-Stage Larvae to Egg-Laying Adults

1992 ◽  
Vol 49 (2) ◽  
pp. 347-348 ◽  
Author(s):  
C. G. Likely ◽  
M. D. B. Burt
Keyword(s):  
Gas Phase ◽  
Culture System ◽  
Sexual Maturity ◽  
Gadus Morhua ◽  
Egg Laying ◽  
In Vitro Cultivation ◽  
Third Stage ◽  
Contracaecum Osculatum

Third-stage (L3) Contracaecum sp. larvae, from Gadus morhua, developed to sexual maturity in a two-step roller culture system using medium API-1 supplemented with 5 mM L-cysteine only (step 1) and with bovine haem (Fildes' reagent) added (step 2). The gas phase was 85% N2 – 10% CO2 – 5% O2 and the culture was maintained at 35 °C. The mature nematodes proved to be Contracaecum osculatum, a species which normally matures in the stomach of pinnipeds.

Cultivation of Pseudoterranova decipiens (Sealworm) from Third-Stage Larvae to Egg-Laying Adults in Vitro

1989 ◽  
Vol 46 (7) ◽  
pp. 1095-1096 ◽  
Author(s):  
C. G. Likely ◽  
M. D. B. Burt
Keyword(s):  
Gas Phase ◽  
Culture System ◽  
Gadus Morhua ◽  
Egg Laying ◽  
Pseudoterranova Decipiens ◽  
Third Stage

Third-stage larvae (L3) of Pseudoterranova decipiens from cod (Gadus morhua) developed to maturity at 35 °C in a two-step roller culture system. The medium was API-1 for step 1, and API-1 with bovine haem supplement for step 2. The gas phase was 85% N: 10% CO2:5% O2.

scholarly journals Angiostrongylus cantonensis (Nematode: Metastrongiloidea): In Vitro Cultivation of Infective Third-Stage Larvae to Fourth-Stage Larvae

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e72084 ◽  
Author(s):  
Rong-Jyh Lin ◽  
Jie-Wen He ◽  
Li-Yu Chung ◽  
June-Der Lee ◽  
Jiun-Jye Wang ◽  
...  
Keyword(s):  
Angiostrongylus Cantonensis ◽  
In Vitro Cultivation ◽  
Fourth Stage ◽  
Third Stage

scholarly journals In vitro cultivation of Toxocara cati adult worms for production of eggs and evaluation of oviposition

2009 ◽  
Vol 46 (1) ◽  
pp. 28-30 ◽  
Author(s):  
M. Zibaei ◽  
S. Sadjjadi ◽  
B. Sarkari ◽  
A. Oryan ◽  
S. Uga
Keyword(s):  
Culture Method ◽  
Toxocara Canis ◽  
Clinical Syndrome ◽  
Egg Laying ◽  
In Vitro Cultivation ◽  
Toxocara Cati ◽  
Second Stage ◽  
Immunological Diagnosis ◽  
The Mean

AbstractToxocariasis is the clinical syndrome caused by infection of zoonotic roundworms of dogs (Toxocara canis) or cats (Toxocara cati). Current research on the immunology and pathology aspects of toxocariasis requires Toxocara second stage larvae and a ready source of excretory-secretory (ES) antigens. We cultured eleven pairs of both sexes of Toxocara cati adult worms maintained in RPMI 1640 medium in order to evaluate the amounts and duration of egg laying. At the first day and last day (day 19), the mean egg counts were 9300 and 250 eggs/ml, respectively. These results showed that this culture method is very appropriate for collection of pure oviposited eggs and/or production of adult ES antigens of Toxocara cati that could be used for immunological diagnosis of toxocariasis.

In vitro cultivation of Hysterothylacium aduncum (Nematoda: Anisakidae) from 3rd-stage larvae to egg-laying adults

Parasitology ◽  
2002 ◽  
Vol 125 (5) ◽  
pp. 467-475 ◽  
Author(s):  
L. IGLESIAS ◽  
A. VALERO ◽  
L. GÁLVEZ ◽  
R. BENÍTEZ ◽  
F. J. ADROHER
Keyword(s):  
Adult Stage ◽  
Defined Medium ◽  
Egg Laying ◽  
Biological Study ◽  
In Vitro Cultivation ◽  
Adult Males ◽  
Average Survival ◽  
The Mean ◽  
Hysterothylacium Aduncum

This is the first demonstration of the in vitro development of the 3rd-stage larvae (L3) of Hysterothylacium aduncum to the adult. This was achieved in a semi-defined medium that is easy to prepare and to reproduce. The L3, collected from the peritoneal cavity of horse mackerel (Trachurus trachurus), were individually inoculated into RPMI-1640 medium +20% heat-inactivated fetal bovine serum (IFBS). It has been demonstrated that the optimum temperature for development is around 13 °C and is stimulated by the presence of 5% CO2 in the growth atmosphere, increasing the percentage moulting to the 4th larval stage (L4) by 1.9-fold (from 44 to 82%) and the average survival of the nematodes by 1.6 times (from 60 to 96 days). When the larvae were grown at different pHs, optimum development occurred at pH 4.0. Under these conditions, all the larvae moulted to the L4 and more than two-thirds transformed to the adult stage – in which 25–30% of the females laid eggs – and reached an average survival of over 4 months. When this medium was supplemented with 1% (w/v) of commercial pepsin, all the larvae reached the adult stage, at least 45% of the females oviposited, laying around 12-fold more eggs per female than in the medium without pepsin. The mean size of the eggs (non-fertilized) obtained was 56.8×47.6 μm. The mean length of the adult males obtained was between 3.2 and 5.2 cm and the females were between 3.0 and 6.5 cm. The adult specimens were morphologically identified as Hysterothylacium aduncum aduncum. This culture medium (RPMI-1640+20% (v/v) IFBS+1% commercial pepsin, at pH 4.0, 13 °C and 5% CO2 in air) could facilitate the identification of at least some of the larvae of the genus Hysterothylacium – and perhaps other anisakids – for which the specific identification and the biological study of these parasites is often difficult.

Evaluation of Different Medium Supplements for In vitro Cultivation of Brugia malayi Third-Stage Larvae

1994 ◽  
Vol 80 (3) ◽  
pp. 380 ◽  
Author(s):  
Cathy L. Smillie ◽  
Ann C. Vickery ◽  
B. H. Kwa ◽  
Jai K. Nayar ◽  
Undara R. Rao
Keyword(s):  
Brugia Malayi ◽  
In Vitro Cultivation ◽  
Third Stage
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