Molecular mapping of the shrunken endosperm genes seg8 and sex1 in barley (Hordeum vulgare L.)

Genome ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 1209-1214 ◽  
Author(s):  
Marion S. Röder ◽  
Christian Kaiser ◽  
Winfriede Weschke
Keyword(s):  
Hordeum Vulgare ◽  
Microsatellite Markers ◽  
Linkage Mapping ◽  
Centromeric Region ◽  
Molecular Mapping ◽  
Recessive Gene ◽  
Rice Chromosome ◽  
Chromosome 2 ◽  
Hordeum Vulgare L ◽  
Causal Genes

A number of mutations affecting seed development in barley (Hordeum vulgare L.) have been known for many years; however, to date, no research has been reported that elucidates the molecular structure of the causal genes. As a first step, we initiated the linkage mapping of the two shrunken endosperm genes seg8 and sex1 using microsatellite markers. The recessive gene seg8 was mapped in the centromeric region of chromosome 7H to a 4.6 cM interval flanked by markers GBM1516 and Bmag341. The recessive sex1 gene showed xenia effects and was located in the centromeric region of barley chromosome 6H, which is in accordance to the previously reported chromosomal location in the classical linkage map. It was flanked by markers GBM5012 and GBM1063 in a 4.2 cM interval. EST-derived microsatellite markers were used to establish the syntenic relationships to the genomic rice sequences. Two orthologous sites on rice chromosome 2 flanking a 4.1 Mb sequence had homology to the respective barley markers in the sex1 region. For the markers in the seg8 region orthologous sites on rice chromosome 6 were detected.

Genetic studies on net blotch resistance in a barley cross

1996 ◽  
Vol 76 (4) ◽  
pp. 715-719 ◽  
Author(s):  
K.M. Ho ◽  
T.M. Choo ◽  
A. Tekauz ◽  
R.A. Martin
Keyword(s):  
Hordeum Vulgare ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Pyrenophora Teres ◽  
Chromosome 2 ◽  
Net Blotch ◽  
Hordeum Vulgare L ◽  
Key Words Barley ◽  
Recessive Genes ◽  
Selection For

An investigation was initiated to study the genetics of resistance to three isolates of Pyrenophora teres (WRS102, WRS858, and WRS857), which have been routinely used for screening for net blotch resistance in Canada. The F1, F2, and doubled-haploid lines were derived from a Leger/CI9831 cross of barley (Hordeum vulgare L.). These materials, along with their parents, were inoculated with each of the three isolates at the three-leaf stage in growth chambers. Results showed that resistance to WRS102 was controlled by three recessive genes, resistance to WRS858 by one recessive gene, and resistance to WRS857 by either one dominant gene or two complementary genes. One of the WRS102-resistance genes appeared to be on chromosome 2 and another linked to the WRS858-resistance gene. Resistance to these three isolates was not associated with awn type, esterase 1, and esterase 5. Selection for resistance to WRS102 and WRS858 would be more effective than selection for resistance to WRS857 in a conventional breeding program. Key words: Barley, Hordeum vulgare, net blotch, Pyrenophora teres, haploids

INHERITANCE AND LINKAGE STUDIES WITH THE GRANDPA GENE IN BARLEY, HORDEUM VULGARE L.

1971 ◽  
Vol 13 (3) ◽  
pp. 489-498
Author(s):  
R. W. Matchett ◽  
H. G. Nass ◽  
D. W. Robertson
Keyword(s):  
Hordeum Vulgare ◽  
Chromosomal Location ◽  
Gene Interactions ◽  
Chromosome 2 ◽  
Linkage Studies ◽  
Hordeum Vulgare L ◽  
Barley Genome ◽  
Chlorophyll Mutants ◽  
Mutant Genes ◽  
Linkage Association

This study was initiated to determine the chromosomal location of the grandpa (gp) gene within the barley genome. The gp gene was placed on the long arm of chromosome 2 as indicated by linkage association with liguleless (li).Tests of allelism showed the gp gene to the allelic with the gp-2 gene. Seven sources of "yellow" chlorophyll mutants when crossed to grandpa plants gave albino double recessive seedlings. Three other sources of "yellow" chlorophyll mutants in the double recessive combination with grandpa exhibited yellow and white bands on the leaves. Double recessive individuals carrying the mottled (mt2) and grandpa genes were also albino. This is evidence of gene interactions between chlorophyll mutant genes.

IDENTICAL GENES FOR VIRULENCE IN THE SMUTS USTILAGO HORDEI AND U. NIGRA, AND INHERITANCE OF VIRULENCE ON THE BARLEY CULTIVARS KEYSTONE AND CONQUEST

1974 ◽  
Vol 54 (2) ◽  
pp. 253-257 ◽  
Author(s):  
R. A. FULLERTON ◽  
J. NIELSEN
Keyword(s):  
Hordeum Vulgare ◽  
Virulence Genes ◽  
Recessive Gene ◽  
Breeding Program ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Ustilago Hordei

Virulence of Ustilago hordei (Pers.) Lagerh. and U. nigra Tapke on the barley (Hordeum vulgare L.) cultivars Keystone and Conquest was studied. One recessive gene or linked genes, the same in both smut species, appeared to confer virulence on both cultivars. Earlier investigators had found identical virulence genes in both species of smut for the cultivars Excelsior, Lion, Pannier and Trebi. Therefore, testing for resistance or susceptibility in a breeding program could be done with a strain of only one of the species.

Inheritance and qualitative analysis of pigments in a barley mutant

1971 ◽  
Vol 49 (1) ◽  
pp. 49-51 ◽  
Author(s):  
Kari Stephansen ◽  
Saul Zalik
Keyword(s):  
Quantitative Analysis ◽  
Hordeum Vulgare ◽  
Qualitative Analysis ◽  
Chlorophyll A ◽  
Recessive Gene ◽  
Single Recessive Gene ◽  
Hordeum Vulgare L ◽  
Chlorophyll Deficiency ◽  
Reciprocal Crosses ◽  
Barley Mutant

Reciprocal crosses were performed with barley (Hordeum vulgare L. cv. ‘Gateway’) and a virescens mutant derived from it. The results from F1 and F2 generations of the crosses showed that chlorophyll deficiency was governed by a single recessive gene. Quantitative analysis of pigment concentrations in normal and mutant shoots of F1 and F2 plants of the same age provided evidence for heterosis in chlorophyll a synthesis.

Acrotrisomic analysis in linkage mapping in barley (Hordeum vulgare L.)

1984 ◽  
Vol 68 (5) ◽  
pp. 433-439 ◽  
Author(s):  
T. Tsuchiya ◽  
R. J. Singh ◽  
Azizeh Shahla ◽  
A. Hang
Keyword(s):  
Hordeum Vulgare ◽  
Linkage Mapping ◽  
Hordeum Vulgare L

Physical mapping of four sites of 5S rDNA sequences and one site of the α-amylase-2 gene in barley (Hordeum vulgare)

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 517-523 ◽  
Author(s):  
I. J. Leitch ◽  
J. S. Heslop-Harrison
Keyword(s):  
In Situ Hybridization ◽  
Hordeum Vulgare ◽  
Physical Mapping ◽  
5S Rdna ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Hordeum Vulgare L ◽  
Rdna Sequences ◽  
5S Rdna Sequence

The 5S rDNA sequences have been mapped on four pairs of barley (Hordeum vulgare L.) chromosomes using in situ hybridization and barley monotelotrisomic lines. The 5S rDNA sequences are located, genetically and physically, on the short arm of chromosome 1 (7I) and the long arms of chromosomes 2 (2I) and 3 (3I). The 5S rDNA sequence is also located on the physically long arm of chromosome 4 (4I). Only one site on chromosome 2(2I) has previously been reported. The characteristic locations of the 5S rDNA sequences make them useful as molecular markers to identify each barley chromosome. The physical position of the low-copy α-amylase-2 gene was determined using in situ hybridization; the location of this gene on the long arm of chromosome 1 (7I) was confirmed by reprobing the same preparation with the 5S rDNA probe. The results show that there is a discrepancy between the physical and genetic position of the α-amylase-2 gene.Key words: genetic mapping, physical mapping, barley, mapping, 5S DNA, α-amylase, in situ hybridization.

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