DEMONSTRATION OF A PARASEXUAL CYCLE IN THE ENTOMOPATHOGENIC FUNGUS METARHIZIUM ANISOPLIAE

1980 ◽  
Vol 22 (2) ◽  
pp. 309-314 ◽  
Author(s):  
K. Al-Aidroos
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Parasexual Cycle

In an attempt to demonstrate the existence of a parasexual cycle in the imperfect entomopathogenic fungus, Metarhizium anisopliae (Metschnikoff) Sorokin, doubly- and triply-marked strains were forced to form heterokaryons. All heterokaryons produced diploid spores, two of which yielded putative recombinants.

scholarly journals Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

1995 ◽  
Vol 52 (3) ◽  
pp. 548-554 ◽  
Author(s):  
V. Kava - Cordeiro ◽  
E.A. Luna - Alves - Lima ◽  
J.L. Azevedo
Keyword(s):  
Gamma Radiation ◽  
Ultraviolet Light ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Nitrous Acid ◽  
Wild Strain ◽  
Wild Type ◽  
Survival Curves ◽  
Auxotrophic Mutants ◽  
Morphological Mutants

A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.

Studies of the inheritance of virulence in the entomopathogenic fungus Metarhizium anisopliae

1985 ◽  
Vol 46 (1) ◽  
pp. 20-25 ◽  
Author(s):  
G. Riba ◽  
J.L. de Azevedo ◽  
C. Messias ◽  
W. Dias da Silveira ◽  
R. Tuveson
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus

The effects of isothiocyanates on the growth of the entomopathogenic fungus Metarhizium anisopliae and its infection of the mustard beetle

1999 ◽  
Vol 103 (8) ◽  
pp. 974-980 ◽  
Author(s):  
E.N. Inyang ◽  
T.M. Butt ◽  
K.J. Doughty ◽  
A.D. Todd ◽  
S. Archer
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus

scholarly journals Screening for attractants compatible with entomopathogenic fungus Metarhizium anisopliae for use in thrips management

2016 ◽  
Vol 15 (17) ◽  
pp. 714-721 ◽  
Author(s):  
Kupesa Mfuti David ◽  
Subramanian Sevgan ◽  
Niassy Saliou ◽  
Salifu Daisy ◽  
du Plessis Hannalene ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus

scholarly journals Sensibility of the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin to Pelargonium sidoides extract (EPs 7630) assessed by conidia germination speed parameter

2014 ◽  
Vol 13 (7) ◽  
pp. 821-826
Author(s):  
Tognolli de Almeida Tiago ◽  
Casarotti Orlandelli Ravely ◽  
Specian Vnia ◽  
Cesar Polonio Julio ◽  
Andressa Lino Loureno Daniela ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Conidia Germination ◽  
Pelargonium Sidoides ◽  
Germination Speed ◽  
Speed Parameter

scholarly journals Protein kinases in the entomopathogenic fungus Metarhizium anisopliae

1990 ◽  
Vol 136 (7) ◽  
pp. 1401-1411 ◽  
Author(s):  
R. J. S. Leger ◽  
L. B. Laccetti ◽  
R. C. Staples ◽  
D. W. Roberts
Keyword(s):  
Protein Kinases ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus

Expression and characterization of the 42 kDa chitinase of the biocontrol fungusMetarhizium anisopliaeinEscherichia coli

2003 ◽  
Vol 49 (11) ◽  
pp. 723-726 ◽  
Author(s):  
César Milton Baratto ◽  
Marcia Vanusa da Silva ◽  
Lucélia Santi ◽  
Luciane Passaglia ◽  
Irene Silveira Schrank ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Expression Vector ◽  
Hydrolytic Enzymes ◽  
Active Protein ◽  
Chitinase Genes

Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase.Key words: Metarhizium anisopliae, chitinases, chit genes, recombinant protein, enthomopathogenic fungi.

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