Banding differences between tiger salamander and axolotl chromosomes

1985 ◽  
Vol 27 (5) ◽  
pp. 510-514 ◽  
Author(s):  
Robert Cuny ◽  
George M. Malacinski
Keyword(s):  
Nucleolar Organizer ◽  
Ambystoma Mexicanum ◽  
Chromosome 9 ◽  
Ambystoma Tigrinum ◽  
Tiger Salamander ◽  
Species Hybrid ◽  
Hoechst 33258 ◽  
Banding Patterns ◽  
Heterogametic Sex ◽  
Species Hybrids

The Hoechst 33258 – Giemsa banding patterns were compared on axolotl (Ambystoma mexicanum Shaw) and axolotl – tiger salamander (Ambystoma tigrinum Green) species hybrid prophase chromosomes. Approximately 369 bands per haploid chromosome set were seen in the axolotl and about 344 bands in the tiger salamander. In the haploid set of 14 chromosomes, chromosome 3 has a constant short or q-arm terminal constriction at the location of the nucleolar organizer. Chromosomes 14 Z and W carry the sex determinants, the female being the heterogametic sex (ZW). The banding patterns of chromosomes 1, 6, 11, and 14 Z of the two species are apparently indistinguishable by our banding method. In the axolotl, chromosome 9 has a small long or p-arm terminal deletion. In the tiger salamander, the remaining 10 chromosomes have terminal or internal deletions. No translocations or inversions seem to have occurred since the gene pool separation of the two closely related species.Key words: chromosome banding, species hybrids, axolotl, tiger salamander.

Cutaneous mastocytomas in the neotenic caudate amphibians Ambystoma mexicanum (axolotl) and Ambystoma tigrinum (tiger salamander)

1999 ◽  
Vol 125 (3-4) ◽  
pp. 187-192 ◽  
Author(s):  
John C. Harshbarger ◽  
Sing Chen Chang ◽  
Louis E. DeLanney ◽  
Francis L. Rose ◽  
David E. Green
Keyword(s):  
Ambystoma Mexicanum ◽  
Ambystoma Tigrinum ◽  
Tiger Salamander

scholarly journals Short-term storage of tiger salamander (Ambystoma tigrinum) spermatozoa: The effect of collection type, temperature and time

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245047
Author(s):  
Amanda B. Gillis ◽  
Emmet L. Guy ◽  
Andrew J. Kouba ◽  
Peter J. Allen ◽  
Ruth M. Marcec-Greaves ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Hormone Treatment ◽  
Effect Of Temperature ◽  
Initial Assessment ◽  
Ambystoma Tigrinum ◽  
Tiger Salamander ◽  
Storage Duration ◽  
Spermatozoa Motility ◽  
Over Time

The aims of this project were to characterize tiger salamander (Ambystoma tigrinum) spermatozoa motility over time, when excreted as either milt or spermic urine prior to packaging into a spermatophore, and to determine the effect of temperature on sperm motility. A split-plot design was utilized to assess the motility of the two pre-spermatophore sample types at two temperatures, 0°C and 20°C (n = 10 for each treatment). Spermiation was induced through exogenous hormone treatment of luteinizing hormone releasing hormone analog in order to collect both milt and spermic urine, which were evaluated for motility, divided into two separate aliquots, and subsequently stored in either an ice-bath (0°C) or on the benchtop (20°C). The decay rate of sperm motility was assessed by reevaluating subsamples at 0.5, 1, 2, 3, 5, 7, and 24 hours following the initial assessment. Results showed that sperm stored at 0°C had significantly higher progressive, non-progressive, and total motility for both sperm collection types over time. An interaction was found between collection type and time, with milt exhibiting lower initial motility that was more sustainable over time, compared to spermic urine. For both milt and spermic urine, motility decreased rapidly with storage duration, indicating samples should be used as soon as possible to maximize motility for in-vitro fertilization and cryopreservation. This is the first study to describe the differences in sperm motility between milt and spermic urine from an internally fertilizing caudate and demonstrates the benefits of near freezing temperatures on sperm longevity.

Pattern of X-Y chromosome pairing in the Taiwan vole, Microtus kikuchii

Genome ◽  
2001 ◽  
Vol 44 (1) ◽  
pp. 27-31 ◽  
Author(s):  
K Mekada ◽  
M Harada ◽  
L K Lin ◽  
K Koyasu ◽  
P M Borodin ◽  
...  
Keyword(s):  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Sex Chromosomes ◽  
Meiotic Prophase ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
The Other ◽  
Banding Patterns ◽  
Y Chromosomes

Pairing of X and Y chromosomes at meiotic prophase and the G- and C-banding patterns and nucleolar organizer region (NOR) distribution were analyzed in Microtus kikuchii. M. kikuchii is closely related to M. oeconomus and M. montebelli, karyologically and systematically. The formation of a synaptonemal complex between the X and Y chromosomes at pachytene and end-to-end association at diakinesis – metaphase I are only observed in three species in the genus Microtus; M. kikuchii, M. oeconomus, and M. montebelli. All the other species that have been studied so far have had asynaptic X–Y chromosomes. These data confirm that M. kikuchii, M. oeconomus, and M. montebelli are very closely related, and support the separation of asynaptic and synaptic groups on the phylogenetic tree.Key words: Microtus kikuchii, Microtus phylogeny, karyotype, synaptic sex chromosomes, synaptonemal complex.

Variation in rDNA Redundancy Level and Nucleolar Organizer Length in Normal and Variant Lines of the Mexican Axolotl

1974 ◽  
Vol 15 (2) ◽  
pp. 239-257
Author(s):  
J. H. SINCLAIR ◽  
CAROLE R. CARROLL ◽  
R. R. HUMPHREY
Keyword(s):  
Genetic Material ◽  
Nucleolar Organizer ◽  
Ambystoma Mexicanum ◽  
The Other ◽  
Colour Pattern ◽  
Nucleolar Organizer Regions ◽  
Wild Type ◽  
Mexican Axolotl ◽  
Apparent Lack ◽  
Heterozygous Condition

The level of redundancy of ribosomal genes, and the relationship of this level to nucleolar formation at different stages of embryonic development, have been examined in the Mexican axolotl, Ambystoma mexicanum. Individuals from 4 inbred stocks were examined, as well as descendants from 2 nucleolar variants which, in the heterozygous condition, are distinguished by exceptionally small nucleoli. Ribosomal RNA-DNA hybridization assays show that one of the 4 wild type lines has only about one-third as much ribosomal DNA (rDNA) as the other three. One of the nucleolar variants has the same level of rDNA as the larger wild-type level; the other variant has the same amount as the smaller ribosomal genome line. Both original nucleolar variants arose as F1 progeny of crosses between a large rDNA genome line and the small genome line. Cytological examination of pregastrula stage embryos from wild type and nucleolar variant lines show that the lengths of the nucleolar organizer regions (NOR) and the sizes of nucleoli formed, are directly correlated with the amount of rDNA present at the nucleolar locus. During gastrulation of the nucleolar variants, however, a transition appears to take place and the amount of rDNA ceases to be the determining factor in nucleolar size. After late gastrula, heterozygous progeny resulting from crosses of either large rDNA genome or small rDNA genome wild type individuals with either nucleolar variant line, have a small and a large nucleolus. The factor or factors associated with this apparent lack of competitive ability of the variant NOR, when opposed to a normal NOR, are unknown. It might be suggested that since the chromosomal alterations which produced the nucleolar variants in both cases eliminated the gene determining the dark colour pattern, they could at the same time have eliminated other genetic material.

Microsatellite mutation rates in the eastern tiger salamander (Ambystoma tigrinum tigrinum) differ 10-fold across loci

Genetica ◽  
2008 ◽  
Vol 136 (3) ◽  
pp. 501-504 ◽  
Author(s):  
Zafer Bulut ◽  
Cory R. McCormick ◽  
David Gopurenko ◽  
Rod N. Williams ◽  
David H. Bos ◽  
...  
Keyword(s):  
Mutation Rates ◽  
Ambystoma Tigrinum ◽  
Tiger Salamander ◽  
Microsatellite Mutation ◽  
Ambystoma Tigrinum Tigrinum

CHROMOSOME BANDING HOMOLOGIES OF A TANDEM FUSION IN RIVER, SWAMP, AND CROSSBRED BUFFALOES (BUBALUS BUBALIS)

1982 ◽  
Vol 24 (6) ◽  
pp. 667-673 ◽  
Author(s):  
T. A. Bongso ◽  
M. Hilmi
Keyword(s):  
First Generation ◽  
Bubalus Bubalis ◽  
Chromosome 9 ◽  
Chromosome 4 ◽  
Centromere Region ◽  
Hybrid Female ◽  
Banding Patterns ◽  
C Banding ◽  
Tandem Fusion ◽  
Y Chromosomes

The chromosomes of the Murrah (River), Swamp (Malaysian kerbau), F1 hybrid (Murrah × Swamp) and first generation backcross (F1 hybrid female × Murrah male) buffaloes (Bubalus bubalis L.) were studied using Giemsa (G) and centromeric (C) banding techniques. The diploid chromosome number for the Murrah was 2n = 50, Swamp 2n = 48, F1 hybrid 2n = 49 and two backcross animals had 2n = 49 and 2n = 50, respectively. The largest two metacentric chromosomes of the Swamp resulted from a tandem fusion between the two chromosomes 4p and 9, respectively, of the Murrah karyotype. The F1 hybrid (2n = 49) and one of the backcrosses (2n = 49) had karyotypes intermediate to the Murrah and Swamp parents. The C banding patterns were useful in identifying the X and Y chromosomes of the buffalo and demonstrated that a major portion of the centromere region of chromosome 9 was not incorporated into chromosome 4 during the tandem fusion.

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