Amplification of Ac in tomato is correlated with high Ac transposition activity

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 265-276 ◽  
Author(s):  
Peter W. Peterson ◽  
John I. Yoder
Keyword(s):  
Transposable Element ◽  
Single Copy ◽  
Tomato Plants ◽  
Transposition Activity ◽  
Transgenic Tomato Plants ◽  
Activity State ◽  
Ac Transposition ◽  
Low Activity ◽  
Different Levels ◽  
Maize Transposable Element

We have assayed the transposition activity of the maize transposable element Ac in transgenic tomato plants that had a single copy of Ac. We found that Ac elements were in either a high or low activity state and that an Ac insertion could cycle from low to high activity within a generation. The different transposition activities were not simply due to the chromosomal position of the element, because the same Ac insertion had different levels of activity in sibling plants. Transposition activity was measured by two methods, one genetic and one physical; both assays gave similar results for each plant studied. Notably, plants with active Ac elements had progeny with amplified Ac copy number, while no amplification was detected in lines containing Ac in a low activity state. Analysis of lines with amplified elements revealed that the elements could be either clustered or dispersed. Our results were consistent with amplification being the result of transposition.Key words: Ae, transposable element, amplification, transposition.

Ac transposition in transgenic tomato plants

1988 ◽  
Vol 213 (2-3) ◽  
pp. 291-296 ◽  
Author(s):  
John I. Yoder ◽  
Joe Palys ◽  
Kevin Alpert ◽  
Michael Lassner
Keyword(s):  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Ac Transposition

Dark respiration rate of transgenic tomato plants expressing FeSOD1 gene under chloride and sulfate salinity

2014 ◽  
Vol 40 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Ye. N. Baranova ◽  
E. N. Akanov ◽  
A. A. Gulevich ◽  
L. V. Kurenina ◽  
S. A. Danilova ◽  
...  
Keyword(s):  
Respiration Rate ◽  
Dark Respiration ◽  
Transgenic Tomato ◽  
Dark Respiration Rate ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Genetic transactivation of Dissociation elements in transgenic tomato plants

1989 ◽  
Vol 218 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Michael W. Lassner ◽  
Joseph M. Palys ◽  
John I. Yoder
Keyword(s):  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Overexpression of LeNHX2 and SlSOS2 increases salt tolerance and fruit production in double transgenic tomato plants

2019 ◽  
Vol 135 ◽  
pp. 77-86 ◽  
Author(s):  
Mourad Baghour ◽  
Francisco Javier Gálvez ◽  
M. Elena Sánchez ◽  
M. Nieves Aranda ◽  
Kees Venema ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Fruit Production ◽  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

scholarly journals Control of Ethylene Synthesis by Expression of a Bacterial Enzyme in Transgenic Tomato Plants

1991 ◽  
Vol 3 (11) ◽  
pp. 1187 ◽  
Author(s):  
Harry J. Klee ◽  
Maria B. Hayford ◽  
Keith A. Kretzmer ◽  
Gerard F. Barry ◽  
Ganesh M. Kishore
Keyword(s):  
Transgenic Tomato ◽  
Ethylene Synthesis ◽  
Tomato Plants ◽  
Bacterial Enzyme ◽  
Transgenic Tomato Plants

scholarly journals Construction of CRISPR/Cas9 expression vectors habouring gRNA targeted on SLIAA9 gene of tomato

2020 ◽  
Vol 18 (1) ◽  
pp. 147-156
Author(s):  
Bui Manh Minh ◽  
Ha Hong Hanh ◽  
Le Thi Thu Hien ◽  
Huynh Thi Thu Hue
Keyword(s):  
Transgenic Plants ◽  
Genome Editing ◽  
Tomato Fruit ◽  
Expression System ◽  
Secondary Compounds ◽  
Expression Vectors ◽  
Tomato Plants ◽  
Normal Morphology ◽  
Transgenic Tomato Plants ◽  
Pcr Test

Tomato (Solanum lycopersicum) is a nutritious fruit containing many secondary compounds with health benefits. The formation of tomato fruit through fertilization is controlled by auxin through Aux/IAA9 and ARF8 proteins. The mutated SlIAA9 gene leads to the parthenocarpic development of fruit or seedless tomato fruit. Nowadays, the CRISPR/Cas9 genome editing system is becoming increasingly popular in modifying desired genes on plant objects. In this study, gRNAs which target on tomato SlIAA9 gene were designed and inserted into CRISPR/Cas9 vectors. In addition, two strains of A. tumefaciens harboring pRGEB31-IAA9G2 and pRGEB32-IAA9G2 vectors carrying CRISPR/Cas9 expression system towards SlIAA9 gene in tomato were successfully created. The strain of A. tumefaciens harboring pRGEB31- IAA9G2 plasmid was used to develop transgenic tomato plants from Micro-Tom variety. PCR test showed that 5/14 plants had the presence of Cas9 gene in T0 plants. The transgenic plants have a normal morphology in comparation with the controls. The evaluation of mutant efficiency, type, and stability of mutations on the SlIAA9 will be conducted on next-generation plants when the mutations are stable and segregated into descendents.

Production of transgenic tomato plants with the Fe-dependent superoxide dismutase gene

2009 ◽  
Vol 35 (4) ◽  
pp. 223-226 ◽  
Author(s):  
E. K. Serenko ◽  
V. N. Ovchinnikova ◽  
L. V. Kurenina ◽  
E. N. Baranova ◽  
A. A. Gulevich ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Superoxide Dismutase Gene

scholarly journals Characterization of theAc/Ds behaviour in transgenic tomato plants using plasmid rescue

1992 ◽  
Vol 20 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Caius M. T. Rommens ◽  
George N. Rudenko ◽  
Paul P. Dijkwel ◽  
Mark J. J. van Haaren ◽  
Pieter B. F. Ouwerkerk ◽  
...  
Keyword(s):  
Transgenic Tomato ◽  
Plasmid Rescue ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Introduction of sense constructs of cinnamate 4-hydroxylase (CYP73A24) in transgenic tomato plants shows opposite effects on flux into stem lignin and fruit flavonoids

2007 ◽  
Vol 68 (11) ◽  
pp. 1497-1509 ◽  
Author(s):  
David J. Millar ◽  
Marianne Long ◽  
Georgina Donovan ◽  
Paul D. Fraser ◽  
Alain-Michel Boudet ◽  
...  
Keyword(s):  
Transgenic Tomato ◽  
Tomato Plants ◽  
Transgenic Tomato Plants ◽  
Stem Lignin

scholarly journals A flare in the optical spotted in the changing-look Seyfert NGC 3516

2020 ◽  
Vol 638 ◽  
pp. A13 ◽  
Author(s):  
D. Ilić ◽  
V. Oknyansky ◽  
L. Č. Popović ◽  
S. S. Tsygankov ◽  
A. A. Belinski ◽  
...  
Keyword(s):  
Maximum Amplitude ◽  
Light Curves ◽  
Continuum Emission ◽  
X Ray ◽  
The Past ◽  
Broad Component ◽  
High Ionization ◽  
Balmer Lines ◽  
Activity State ◽  
Low Activity

Context. We present observations from the short-term intensive optical campaign (from September 2019 to January 2020) of the changing-look Seyfert NGC 3516. This active galactic nucleus is known to have strong optical variability and has changed its type in the past. It has been in the low-activity state in the optical since 2013, with some rebrightening from the end of 2015 to the beginning of 2016, after which it remained dormant. Aims. We aim to study the photometric and spectral variability of NGC 3516 from the new observations in U- and B-bands and examine the profiles of the optical broad emission lines in order to demonstrate that this object may be entering a new state of activity. Methods. NGC 3516 has been monitored intensively for the past 4 months with an automated telescope in U and B filters, enabling accurate photometry of 0.01 precision. Spectral observations were triggered when an increase in brightness was spotted. We support our analysis of past-episodes of violent variability with the UV and X-ray long-term light curves constructed from the archival Swift/UVOT and Swift/XRT data. Results. An increase of the photometric magnitude is seen in both U and B filters to a maximum amplitude of 0.25 mag and 0.11 mag, respectively. During the flare, we observe stronger forbidden high-ionization iron lines ([Fe VII] and [Fe X]) than reported before, as well as the complex broad Hα and Hβ lines. This is especially seen in Hα, which appears to be double-peaked. It seems that a very broad component of ∼10 000 km s−1 in width in the Balmer lines is appearing. The trends in the optical, UV, and X-ray light curves are similar, with the amplitudes of variability being significantly larger in the case of UV and X-ray bands. Conclusions. The increase of the continuum emission, the variability of the coronal lines, and the very broad component in the Balmer lines may indicate that the AGN of NGC 3516 is finally leaving the low-activity state in which it has been for the last ∼3 years.

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