Fluorescent in situ hybridization with rDNA probes on chromosomes of Acipenser ruthenus and Acipenser naccarii (Osteichthyes Acipenseriformes)

The genes for 28S and 5S rDNA were physically mapped on the chromosomes of two sturgeon species, the sterlet (Acipenser ruthenus, 2n = 118 ± 4) and the Adriatic sturgeon (Acipenser naccarii, 2n = 248 ± 4) by fluorescent in situ hybridization. In the sterlet, the 28S rDNA was located on six chromosomes, four of which actively transcribed, while in the Adriatic sturgeon the 28S rDNA was located on a chromosome number ranging from 10 to 12, eight of which actively transcribed. The 5S rDNA was physically mapped on two chromosomes in the sterlet and on four in the Adriatic sturgeon. A more detailed characterization of the latter karyotype was obtained during this study. All these data are discussed in connection with the ploidy relationships among sturgeon species.Key words: karyotype, ploidy, FISH, 28S and 5S rDNA.

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Chromosomal mapping of 18S28S and 5S rRNA genes by two-colour fluorescent in situ hybridization in six sturgeon species

Genome ◽

10.1139/g03-007 ◽

2003 ◽

Vol 46 (3) ◽

pp. 473-477 ◽

Cited By ~ 39

Author(s):

Francesco Fontana ◽

Massimo Lanfredi ◽

Leonardo Congiu ◽

Marilena Leis ◽

Milvia Chicca ◽

...

Keyword(s):

In Situ Hybridization ◽

Close Association ◽

5S Rdna ◽

28S Rdna ◽

5S Rrna ◽

Chromosomal Mapping ◽

Rrna Genes ◽

Acipenser Baerii ◽

Sturgeon Species

The number and distribution of the 18S28S and 5S rRNA (rDNA) gene sequences were examined on mitotic chromosomes of six sturgeon species by two-colour in situ hybridization. Four of the six species, Huso huso, Acipenser stellatus, Acipenser sturio, and Acipenser ruthenus, with about 120 chromosomes, showed from six to eight 18S28S rDNA signals, while 5S rDNA signals were on only one chromosome pair. The two species with 250270 chromosomes, Acipenser baerii and Acipenser transmontanus, showed from 10 to 12 18S28S sites and two chromosome pairs bearing 5S rDNA signals. In all examined species, the rather intense 5S rDNA signals apparently overlapped those of 18S28S rDNA. These data support the diploidtetraploid relationships between the two chromosome groups of sturgeons. The close association between the two rDNA families in species belonging to an ancestral fish order, such as Acipenseriformes, supports the hypothesis that the association represents a primitive condition.Key words: Acipenseriformes, FISH, fish cytogenetics, ribosomal genes.

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More detailed characterization of some of the HL60 karyotypic features by fluorescence in situ hybridization

Cancer Genetics and Cytogenetics ◽

10.1016/0165-4608(95)00214-6 ◽

1996 ◽

Vol 87 (2) ◽

pp. 103-106 ◽

Cited By ~ 8

Author(s):

Emanuela V. Volpi ◽

R. Vatcheva ◽

T. Labella ◽

S.U. Gan

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Detailed Characterization

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Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization

Genome ◽

10.1139/g95-142 ◽

1995 ◽

Vol 38 (6) ◽

pp. 1061-1069 ◽

Cited By ~ 42

Author(s):

A. Cuadrado ◽

N. Jouve ◽

C. Ceoloni

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Repetitive Dna ◽

Dna Sequences ◽

5S Rdna ◽

Individual Identification ◽

Highly Repetitive Dna ◽

The Individual

The molecular characterization of heterochromatin in six lines of rye has been performed using fluorescence in situ hybridization (FISH). The highly repetitive rye DNA sequences pSc 119.2, pSc74, and pSc34, and the probes pTa71 and pSc794 containing the 25S–5.8S–18S rDNA (NOR) and the 5S rDNA multigene families, respectively, were used. This allowed the individual identification of all seven rye chromosomes and most chromosome arms in all lines. All varieties showed similar but not identical patterns. A standard in situ hybridization map was constructed following the nomenclature system recommended for C-bands. All FISH sites observed appeared to correspond well with C-band locations, but not all C-banding sites coincided with hybridization sites of the repetitive DNA probes used. Quantitative and qualitative differences between different varieties were found for in situ hybridization response at corresponding sites. Variation between plants and even between homologous chromosomes of the same plant was found in open-pollinated lines. In inbred lines, the in situ pattern of the homologues was practically identical and no variation between plants was detected. The observed quantitative and qualitative differences are consistent with a corresponding variation for C-bands detected both within and between cultivars.Key words: fluorescence in situ hybridization, repetitive DNA, rye, Secale cereale, polymorphism.

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