Identification of rice blast resistance genes in the elite hybrid rice restorer line Yahui2115

Rice blast, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most serious rice diseases worldwide. We previously developed an elite hybrid rice restorer line with high resistance to rice blast, Yahui2115 (YH2115). To identify the blast resistance genes in YH2115, we first performed expression profiling on previously reported blast resistance genes and disease assay on monogenic lines, and we found that Pi2, Pi9, and Pikm were the most likely resistance candidates in YH2115. Furthermore, RNA interference and linkage analysis demonstrated that silencing of Pi2 reduced the blast resistance of YH2115 and a Pi2 linkage marker was closely associated with blast resistance in an F2 population generated from YH2115. These data suggest that the broad-spectrum blast resistance gene Pi2 contributes greatly to the blast resistance of YH2115. Thus, YH2115 could be used as a new germplasm to facilitate rice blast resistance breeding in hybrid rice breeding programs.

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Identification of novel rice blast resistance alleles through sequence-based allele mining

10.21203/rs.2.18859/v1 ◽

2019 ◽

Author(s):

Ying Zhou ◽

Fang Lei ◽

Qiong Wang ◽

Weicong He ◽

Yuan Bin ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Resistance Breeding ◽

Economic Losses ◽

The Novel ◽

Allele Mining ◽

Rice Varieties ◽

Rice Blast Resistance ◽

Blast Resistance Genes

Abstract Background: As rice ( Oryza sativa ) is the staple food of more than half the world’s population, rice production contributes greatly to global food security. Rice blast caused by the fungus M agnaporthe oryzae is a devastating fungal disease of rice, affecting yield and grain quality and resulting in substantial annual economic losses. Because the fungus evolves rapidly,, resistance conferred by most of the single blast race resistance genes is often broken after a few years of intensive agricultural use. Effective resistance breeding in rice therefore requires continual enrichment of the reservoir of resistance genes and alleles. Seed banks represent a rich source of genetic diversity; however, they have not been extensively used to identify novel genes and alleles. Results: We carried out a large-scale screen for novel blast resistance alleles in 1883 rice varieties from major rice producing areas across China. Of these, 107 varieties showed at least moderate resistance to natural infection by rice blast at rice blast nurseries in Enshi and Yichang, Hubei Province. Using sequence-based allele mining to amplify and clone the allelic variants of major rice blast resistance genes at the Pi2/9/gm/zt locus of chromosome 6 from the 107 blast-resistant varieties, we identified 13 novel blast resistance alleles. We then used controlled infections to assess the resistance of rice varieties carrying the novel alleles to 34 single rice blast isolates from Hubei, Guangdong, Jiangsu, Hunan, Jangxi, Sichuan, Heilongjiang, and Fujin Provinces. The varieties identified as being resistant in the nursery trials showed varied disease responses when infected with the single blast isolates, suggesting that the novel Pi2/9/gm/zt alleles vary in their blast resistance spectra. Some of the newly identified alleles have unique single nucleotide polymorphisms (SNPs), insertions, or deletions, in addition to polymorphic residues that are shared between the different alleles. Conclusions: These alleles expand the allelic series of blast resistance genes, enriching the genetic resource for rice blast resistance breeding programs and for studies aimed at deciphering rice–rice blast molecular interactions. Key words : Pi9 , R-genes, Nucleotide diversity, Gene conversion, Resistance gene alleles, Rice blast

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Identification of Novel Alleles of the Rice Blast-Resistance Gene Pi9 through Sequence-Based Allele Mining

Rice ◽

10.1186/s12284-020-00442-z ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Ying Zhou ◽

Fang Lei ◽

Qiong Wang ◽

Weicong He ◽

Bin Yuan ◽

...

Keyword(s):

Resistance Genes ◽

Tandem Repeat ◽

Rice Blast ◽

Blast Resistance ◽

Resistance Breeding ◽

Economic Losses ◽

Chromosome 6 ◽

Allele Mining ◽

Rice Blast Resistance ◽

Blast Resistance Genes

Abstract Background As rice (Oryza sativa) is the staple food of more than half the world’s population, rice production contributes greatly to global food security. Rice blast caused by the fungus Magnaporthe oryzae (M. oryzae) is a devastating disease that affects rice yields and grain quality, resulting in substantial economic losses annually. Because the fungus evolves rapidly, the resistance conferred by most the single blast-resistance genes is broken after a few years of intensive agricultural use. Therefore, effective resistance breeding in rice requires continual enrichment of the reservoir of resistance genes, alleles, or QTLs. Seed banks represent a rich source of genetic diversity; however, they have not been extensively used to identify novel genes and alleles. Results We carried out a large-scale screen for novel blast-resistance alleles in 1883 rice varieties from major rice-producing areas across China. Of these, 361 varieties showed at least moderate resistance to natural infection by rice blast at rice blast nurseries in Enshi and Yichang, Hubei Province. We used sequence-based allele mining to amplify and sequence the allelic variants of the major rice blast-resistance genes at the Pi2/Pi9 locus of chromosome 6 from the 361 blast-resistant varieties, and the full-length coding region of this gene could be amplified from 107 varieties. Thirteen novel Pi9 alleles (named Pi9-Type1 to Pi9-Type13) were identified in these 107 varieties based on comparison to the Pi9 referenced sequence. Based on the sequencing results, the Pi2/Pi9 locus of the 107 varieties was divided into 15 genotypes (including three different genotypes of Pi9-Type5). Fifteen varieties, each representing one genotype, were evaluated for resistance to 34 M. oryzae isolates. The alleles from seven varieties with the highest resistance and widest resistance spectra were selected for transformation into the susceptible variety J23B to construct near-isogenic lines (NILs). These NILs showed resistance in a field test in Enshi and Yichang, indicating that the seven novel rice blast-resistance tandem-repeat regions at the Pi2/Pi9 locus of chromosome 6 could potentially serve as a genetic resource for molecular breeding of resistance to rice blast. Conclusions The thirteen novel Pi9 alleles identified in this study expand the list of available of blast-resistance alleles. Seven tandem-repeat regions of the Pi2/Pi9 locus from different donors were characterized as broad-spectrum rice blast-resistance fragments; these donors enrich the genetic resources available for rice blast-resistance breeding programs.

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The Utilization of Rice Blast Resistance Genes in Hybrid Rice Breeding in China

Protecting Rice Grains in the Post-Genomic Era ◽

10.5772/intechopen.83617 ◽

2019 ◽

Cited By ~ 1

Author(s):

Junjie Xing ◽

Huafeng Deng ◽

Longping Yuan

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Hybrid Rice ◽

Blast Resistance ◽

Rice Breeding ◽

Rice Blast Resistance ◽

Blast Resistance Genes

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Expression-based genotyping of the rice blast resistance genes in the elite maintainer line Yixiang1B

European Journal of Plant Pathology ◽

10.1007/s10658-017-1149-1 ◽

2017 ◽

Vol 148 (4) ◽

pp. 955-965 ◽

Cited By ~ 1

Author(s):

Li Wang ◽

Xiao-Hong Hu ◽

Gang Lin ◽

De-Ming Zhao ◽

Jun Shi ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Maintainer Line ◽

Rice Blast Resistance ◽

Blast Resistance Genes

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Screening of the Dominant Rice Blast Resistance Genes with PCR-based SNP and CAPS Marker in Aromatic Rice Germplasm

Korean Journal of Crop Science ◽

10.7740/kjcs.2011.56.4.329 ◽

2011 ◽

Vol 56 (4) ◽

pp. 329-341 ◽

Cited By ~ 1

Author(s):

Jeong-Soon Kim ◽

Sang-Nag Ahn ◽

Sung-Jun Hong ◽

Jin-Hyeuk Kwon ◽

Yeong-Ki Kim ◽

...

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Blast Resistance ◽

Aromatic Rice ◽

Caps Marker ◽

Rice Blast Resistance ◽

Rice Germplasm ◽

Blast Resistance Genes

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Identification of rice blast (Pyricularia) resistance genes in the breeding samples of rice

E3S Web of Conferences ◽

10.1051/e3sconf/202017501008 ◽

2020 ◽

Vol 175 ◽

pp. 01008

Author(s):

Nataliya Vozhzhova ◽

Elena Krasnova ◽

Elena Ionova

Keyword(s):

Resistance Genes ◽

Rice Blast ◽

Agricultural Research ◽

Blast Resistance ◽

Genetic Material ◽

Climatic Conditions ◽

Rice Blast Resistance ◽

Rice Samples ◽

Functional Alleles ◽

Blast Resistance Genes

The current paper has considered the challenge of breeding of rice lines adapted to the climatic conditions of the Southern Federal District of Russia by pyramiding a complex of Pi-1, Pi-2, Pi-33, Pi-ta and Pi-b rice blast resistance genes which is efficient in the southern part of Russia. The control of the resistance genes present in the breeding material was carried out using a marker-associated selection (MAS). The purpose of the current study was to identify the Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b rice blast resistance genes in the rice samples developed in the laboratory of rice breeding and seed production of the FSBSI “Agricultural Research Center “Donskoy” to identify valuable genotypes. The screening of 689 breeding samples of rice resulted in identification of the allelic state of the Pi-1, Pi-2, Pi-33, Pi-ta and Pi-b rice blast resistance genes, and the genotypes with functional alleles of these genes and their combinations. There were identified 19 breeding samples of rice possessing a combination of 4 functional alleles of rice blast resistance genes in various combinations. The identified rice samples are valuable genetic material and are recommended to use as the sources of rice blast resistance in the breeding process.

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