DNA barcoding the bees (Hymenoptera: Apoidea) of Chile: species discovery in a reasonably well known bee fauna with the description of a new species of Lonchopria (Colletidae)

Genome ◽  
2017 ◽  
Vol 60 (5) ◽  
pp. 414-430 ◽  
Author(s):  
Laurence Packer ◽  
Luisa Ruz
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Morphological Differentiation ◽  
Spanish Language ◽  
Dna Barcodes ◽  
Species Discovery ◽  
Traditional Taxonomy ◽  
Taxonomic Research ◽  
A New Species

We compare the diversity of bees in the Chilean fauna as understood from traditional taxonomy-based catalogues with that currently known from DNA barcodes using the BIN system informed by ongoing morphology-based taxonomic research. While DNA barcode surveys of the Chilean bee fauna remain incomplete, it is clear that new species can readily be distinguished using this method and that morphological differentiation of distinct barcode clusters is sometimes very easy. We assess the situation in two genera in some detail. In Lonchopria Vachal one “species” is readily separable into two BINs that are easily differentiated based upon male mandibular and genitalic morphology (characters generally used in this group) as well as female hair patterns. Consequently, we describe Lonchopria (Lonchopria) heberti Packer and Ruz, new species. For Liphanthus Reed, a large number of new species has been detected using DNA barcoding and considerable additional traditional morphological work will be required to describe them. When we add the number of BINs (whether identified to named species or not) to the number of Chilean bee species that we know have not been barcoded (both described and new species under study in our laboratories) we conclude that the bee fauna of Chile is substantially greater than the 436 species currently known. Spanish language abstract available as supplementary data 1 .

Revision of the Australian Oenochroma vinaria Guenée, 1858 species-complex (Lepidoptera: Geometridae, Oenochrominae): DNA barcoding reveals cryptic diversity and assesses status of type specimen without dissection

Zootaxa ◽  
2009 ◽  
Vol 2239 (1) ◽  
pp. 1-21 ◽  
Author(s):  
AXEL HAUSMANN ◽  
PAUL D. N. HEBERT ◽  
ANDREW MITCHELL ◽  
RODOLPHE ROUGERIE ◽  
MANFRED SOMMERER ◽  
...  
Keyword(s):  
New Species ◽  
Species Complex ◽  
New South ◽  
Dna Barcode ◽  
Type Specimen ◽  
Immature Stages ◽  
Cryptic Diversity ◽  
Dna Barcodes ◽  
South Wales ◽  
A New Species

The assembly of a DNA barcode library for Australian Lepidoptera revealed that Oenochroma vinaria Guenée, 1858, as currently understood, is actually a mix of two different species. By analyzing DNA barcodes from recently collected specimens and the 150 year-old female lectotype of O. vinaria, we propose a reliable assignment of the name vinaria to one of these two species. A lectotype is designated for Monoctenia decora, a confirmed synonym of O. vinaria, and a new species, Oenochroma barcodificata sp. nov., is described. This species is only known from Tasmania and New South Wales; its biology and immature stages are described in detail.

scholarly journals Monopis jussii, a new species (Lepidoptera, Tineidae) inhabiting nests of the Boreal owl (Aegolius funereus)

ZooKeys ◽  
2020 ◽  
Vol 992 ◽  
pp. 157-181
Author(s):  
Marko Mutanen ◽  
Peter Huemer ◽  
Jonna Autto ◽  
Ole Karsholt ◽  
Lauri Kaila
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Parus Major ◽  
Species Discovery ◽  
Aegolius Funereus ◽  
The Alps ◽  
Suitable Habitats ◽  
Boreal Owl ◽  
A New Species ◽  
Ural Owl

Monopis jussii Kaila, Mutanen, Huemer, Karsholt & Autto, sp. nov. (Lepidoptera, Tineidae) is described as a new species. It is closely related to the widespread and common M. laevigella ([Denis & Schiffermüller], 1775), but differs in its distinct COI DNA barcode sequences, four examined nuclear loci as well as details in forewing coloration and pattern. Most reared specimens of M. jussii have emerged from the nest remnants of the Boreal owl (Aegolius funereus (Linnaeus, 1758)), but also nests of the Ural owl (Strix uralensis Pallas, 1771) and the Great tit (Parus major Linnaeus, 1758) have been observed as suitable habitats. Based on the present knowledge, the new species has a boreo-montane distribution as it is recorded only from northern Europe and the Alps. Several extensive rearing experiments from Strix spp. nest remnants from southern Finland did not produce any M. jussii, but thousands of M. laevigella, suggesting that the species is lacking in the area or, more unlikely, that the nest of these owl species do not serve as good habitat for the new species. This unexpected species discovery highlights, once again, the usefulness of DNA barcoding in revealing the cryptic layers of biodiversity. To serve stability we select a neotype for Tinea laevigella [Denis & Schiffermüller], 1775, and discuss the complicated synonymy and nomenclature of this species.

A new species of Rhododendron (Ericaceae) from the Gaoligong Mountains, Yunnan, China, supported by morphological and DNA barcoding data

Phytotaxa ◽  
2013 ◽  
Vol 114 (1) ◽  
pp. 42 ◽  
Author(s):  
YONGPENG MA ◽  
ZHIKUN WU ◽  
RUIJUAN XUE ◽  
XIAOLING TIAN ◽  
LIANMING GAO ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Molecular Analysis ◽  
Dna Barcodes ◽  
Eastern Side ◽  
Mature Leaves ◽  
Morphological Differences ◽  
A New Species

Rhododendron baihuaense sp. nov. is described as a new species endemic to Yunnan, China, that has, to date, only been found in Baihualing on the eastern side of the Gaoligong Mountains. Morphologically, R. baihuaense can be readily distinguished from R. hanceanum and R. genestierianum, by its absent scales on mature leaves, only 2–5 flowers per inflorescence, the funnel-shaped flower and white corolla, and its slender and erect style. A molecular analysis based on the four standard DNA barcodes (rbcL, matK, trnH-psbA and ITS) indicates that R. baihuaense is genetically distinct from R. hanceanum and R. genestierianum, and, together with the morphological differences, is described here as a new species.

scholarly journals Dichrooscytus fervens sp. n., a new species of Miridae (Hemiptera, Heteroptera) from Finland

2019 ◽  
Vol 30 (4) ◽  
pp. 159-167
Author(s):  
Arto Muinonen ◽  
Veikko Rinne ◽  
Eero Vesterinen
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Identification Key ◽  
Coi Gene ◽  
Northern Europe ◽  
Morphological Description ◽  
Dna Barcodes ◽  
A New Species

Dichrooscytus fervens Muinonen & Rinne sp. n. (Hemiptera: Heteroptera: Miridae) is described based on material collected in Finland between 2011 and 2018. A morphological description is provided along with DNA barcodes (COI gene) and a phylogeny of all the Dichrooscytus species for which a DNA barcode is available. The new species lives on Picea spp. and is still known only from Finland. A map of all the records is given, as well as an identification key of the Dichrooscytus species occurring in northern Europe.

A new species of Tanytarsus van der Wulp, 1874 (Diptera: Chironomidae) and the first Japanese record of Tanytarsus ovatus Johannsen, 1932 with DNA barcodes

Zootaxa ◽  
2018 ◽  
Vol 4422 (4) ◽  
pp. 591
Author(s):  
ATSUYA KODAMA ◽  
KOICHIRO KAWAI ◽  
HIDETOSHI SAITO
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Adult Male ◽  
Related Species ◽  
First Record ◽  
Species Group ◽  
Dna Barcodes ◽  
A New Species

A new species of the genus Tanytarsus van der Wulp, 1874 was described and illustrated based on an adult male. Tanytarsus trichovalis sp. nov. belongs to the eminulus species group, and is closely related to T. tamaundecimus Sasa, 1980, T. okuboi Sasa, 1986, and T. tonebeceus Sasa & Tanaka, 2000. However, the species is distinguished from these species by the morphology of the hypopygium. We also present the first record of T. ovatus Johannsen, 1932 in Japan. In addition, DNA barcoding of 5 species including T. trichovalis sp. nov., T. ovatus, and other morphologically related species was undertaken. A new key of the eleven Japanese eminulus species group was compiled. 

scholarly journals Hydrodroma angelieri (Acari, Hydrachnidia: Hydrodromidae) a new water mite species from Corsica based on morphological and DNA barcode evidence

Acarologia ◽  
2022 ◽  
Vol 62 (1) ◽  
pp. 3-11
Author(s):  
Vladimir Pešić ◽  
Harry Smit
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Distinct Species ◽  
Mite Species ◽  
Morphological Data ◽  
Dna Barcodes ◽  
Water Mite ◽  
European Species ◽  
A New Species ◽  
High Genetic Distance

In the present study we used morphological data and DNA barcodes to describe a new species, Hydrodroma angelieri sp. nov. from Corsica, France. A high genetic distance of 17.3±0.017% K2P from its molecularly most closely related European congener, H. despiciens (Müller, 1776), supports H. angelieri sp. nov. as a distinct species. Morphologically the new species can be identified on the basis of relatively small leg claws, the presence of only one swimming seta on II-L-5 and 4-6 swimming setae on the anterior surface of IV-L-5. An updated key for the European species of Hydrodroma is provided.

scholarly journals Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Species Identification ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Primate Species ◽  
Dna Barcodes ◽  
Global Biodiversity ◽  
Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

A new species of the genus Parapentacentrus Shiraki, 1930 from Yunnan, China (Orthoptera: Gryllidae: Itarinae)

Zootaxa ◽  
2021 ◽  
Vol 5032 (1) ◽  
pp. 143-146
Author(s):  
YI-JIAO LIU ◽  
ZHU-QING HE
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Dna Barcode ◽  
Coi Gene ◽  
East China ◽  
Anal Plate ◽  
Type Specimens ◽  
Normal University ◽  
One New Species ◽  
A New Species

The genus Parapentacentrus Shiraki, 1930 includes two species with long wings. In this paper, we report one new species, P. brevipennis He sp. nov., from Jinping, Yunnan, China. The new species have short forewings and hindwings, and have differences in the shape of supra-anal plate and male genitalia. DNA Barcode (COI gene) of this new species are provided. The type specimens are deposited in Museum of Biology, East China Normal University (ECNU).  

scholarly journals A new species of Sacada Walker, 1862 from Thailand (Lepidoptera, Pyralidae, Pyralinae)

2020 ◽  
Vol 4 (2) ◽  
pp. 71-77
Author(s):  
Markku J. Pellinen ◽  
Reza Zahiri ◽  
Pasi Sihvonen
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Valid Species ◽  
Morphological Comparison ◽  
Northern Thailand ◽  
Lepidoptera Pyralidae ◽  
A New Species

A new species of Sacada from northern Thailand is described: S. chaehomensissp. nov. Pellinen & Zahiri (Lepidoptera: Pyralidae, Pyralinae). Morphological characters and DNA barcode data are provided for the new species, with a morphological comparison to S. dzonguensis and S. umtasorensis, and a DNA-barcode comparison to S. ragonotalis and S. albioculalis, respectively. After this addition, the current number of valid species in the genus Sacada is 43.

scholarly journals A cryptic new species of Chlidonoptera Karsch, 1892 from the south west protected zone of the Central African Republic (Insecta, Mantodea, Hymenopodidae)

ZooKeys ◽  
2020 ◽  
Vol 917 ◽  
pp. 63-83
Author(s):  
Nicolas Moulin
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
National Park ◽  
Central African Republic ◽  
Measurement Data ◽  
Critical Issue ◽  
Key To Species ◽  
Tropical Regions ◽  
History Information ◽  
A New Species

Between 1998 and 2012, several scientific expeditions in Dzanga-Sangha Special Reserve and Dzanga-Ndoki National Park led to the collection of many Mantodea specimens from Central African Republic (CAR). Among these specimens, several males of an undescribed species were discovered. Morphologically, this species most closely resembles to Chlidonoptera vexillum Karsch, 1892 and Chlidonoptera lestoni Roy, 1975. A new lineage was revealed by DNA barcoding. Therefore, a new species is described, Chlidonoptera roxanaesp. nov. Habitus images, genitalia illustrations and descriptions, measurement data, a key to species, natural history information, and locality data are provided. These results add to the evidence that cryptic species can be found in tropical regions, a critical issue in efforts to document global species richness. They also illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity.

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