A new species of Rhododendron (Ericaceae) from the Gaoligong Mountains, Yunnan, China, supported by morphological and DNA barcoding data

Phytotaxa ◽  
2013 ◽  
Vol 114 (1) ◽  
pp. 42 ◽  
Author(s):  
YONGPENG MA ◽  
ZHIKUN WU ◽  
RUIJUAN XUE ◽  
XIAOLING TIAN ◽  
LIANMING GAO ◽  
...  

Rhododendron baihuaense sp. nov. is described as a new species endemic to Yunnan, China, that has, to date, only been found in Baihualing on the eastern side of the Gaoligong Mountains. Morphologically, R. baihuaense can be readily distinguished from R. hanceanum and R. genestierianum, by its absent scales on mature leaves, only 2–5 flowers per inflorescence, the funnel-shaped flower and white corolla, and its slender and erect style. A molecular analysis based on the four standard DNA barcodes (rbcL, matK, trnH-psbA and ITS) indicates that R. baihuaense is genetically distinct from R. hanceanum and R. genestierianum, and, together with the morphological differences, is described here as a new species.

Author(s):  
Claudia Isabel Navarro-Rodríguez ◽  
Alejandro Valdez-Mondragón

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.


REINWARDTIA ◽  
2016 ◽  
Vol 14 (2) ◽  
pp. 307 ◽  
Author(s):  
MARLINA ARDIYANI

ARDIYANI, M. 2015. - A new species of Zingiber (Zingiberaceae) from Enggano Island, Indonesia. Reinwardtia 14 (2): 307 - 310. - A species of Zingiber Miller (Zingiberaceae), Z. engganoensis Ardiyani, from Enggano Island, Indonesia is described. The species is only known from its type locality. It is similar to Z. spectabile Griff. but with some morphological differences. Three-locus DNA barcodes (rbcL, matK and ITS2) of the new species were generated for its identification purposes


Zootaxa ◽  
2016 ◽  
Vol 4200 (4) ◽  
pp. 523 ◽  
Author(s):  
FATEMEH HOSSEININAVEH ◽  
JAMASB NOZARI ◽  
MEHMET BORA KAYDAN ◽  
VAHID HOSSEININAVEH

Members of the family Diaspididae (Hemiptera: Coccomorpha) can be devastating pests that suck parenchyma cell contents from crops and cause severe damage to pistachio trees (Pistacia vera L.). The current research collected and characterized diaspidid species from pistachio orchards in Kerman province, Iran, according to their morphological and molecular features. Lepidosaphes pistaciae Archangelskaya, Suturaspis davatchi (Balachowsky & Kaussari) and Melanaspis inopinata (Leonardi) are redescribed and a new species, Melanaspis pistaciae Hosseininaveh & Kaydan sp. n., is described. Phylogenetic trees based on molecular analysis of COI and 28S rDNA fragments placed all the species in separated clades and confirmed M. pistaciae as a new taxon which is concluded by morphological differences. Molecular analysis suggests non-monophyly of the populations of each species. Melanaspis pistaciae sp. n. has spread to most cultivated pistachio areas in Iran and has probably been misidentified as M. inopinata in the past. Further investigation of the biology of this species may lead to development of more effective approaches for controlling this pest. 


Genome ◽  
2017 ◽  
Vol 60 (5) ◽  
pp. 414-430 ◽  
Author(s):  
Laurence Packer ◽  
Luisa Ruz

We compare the diversity of bees in the Chilean fauna as understood from traditional taxonomy-based catalogues with that currently known from DNA barcodes using the BIN system informed by ongoing morphology-based taxonomic research. While DNA barcode surveys of the Chilean bee fauna remain incomplete, it is clear that new species can readily be distinguished using this method and that morphological differentiation of distinct barcode clusters is sometimes very easy. We assess the situation in two genera in some detail. In Lonchopria Vachal one “species” is readily separable into two BINs that are easily differentiated based upon male mandibular and genitalic morphology (characters generally used in this group) as well as female hair patterns. Consequently, we describe Lonchopria (Lonchopria) heberti Packer and Ruz, new species. For Liphanthus Reed, a large number of new species has been detected using DNA barcoding and considerable additional traditional morphological work will be required to describe them. When we add the number of BINs (whether identified to named species or not) to the number of Chilean bee species that we know have not been barcoded (both described and new species under study in our laboratories) we conclude that the bee fauna of Chile is substantially greater than the 436 species currently known. Spanish language abstract available as supplementary data 1 .


Zootaxa ◽  
2018 ◽  
Vol 4422 (4) ◽  
pp. 591
Author(s):  
ATSUYA KODAMA ◽  
KOICHIRO KAWAI ◽  
HIDETOSHI SAITO

A new species of the genus Tanytarsus van der Wulp, 1874 was described and illustrated based on an adult male. Tanytarsus trichovalis sp. nov. belongs to the eminulus species group, and is closely related to T. tamaundecimus Sasa, 1980, T. okuboi Sasa, 1986, and T. tonebeceus Sasa & Tanaka, 2000. However, the species is distinguished from these species by the morphology of the hypopygium. We also present the first record of T. ovatus Johannsen, 1932 in Japan. In addition, DNA barcoding of 5 species including T. trichovalis sp. nov., T. ovatus, and other morphologically related species was undertaken. A new key of the eleven Japanese eminulus species group was compiled. 


Zootaxa ◽  
2019 ◽  
Vol 4612 (2) ◽  
pp. 171 ◽  
Author(s):  
AKIHIKO SHINOHARA ◽  
YUICHI KAMEDA

Taxonomic status of the widely spread xiphydriid woodwasp Xiphydria camelus (Linné, 1758) was revised by examining morphology of 964 specimens and by molecular analysis using COI barcode sequences. Both morphological and molecular approaches indicated existence of three separate species masquerading in X. camelus of authors in East Asia. The three species were finally determined as X. camelus, X. eborata Konow, 1899, and a new species, X. albopicta. Xiphydria eborata is revived from synonymy with X. camelus and X. albopicta is described as a new species from Russia (Primorskij Kraj), China (Heilongjiang) and Japan (Hokkaido). Collection records are given for all the specimens examined. 


2019 ◽  
pp. 23-36
Author(s):  
Mario. R. Cabrera

Formerly Cnemidophorus was thought to be the most speciose genus of Teiidae. This genus comprised four morphological groups that were later defined as four different genera, Ameivula, Aurivela, Cnemidophorus and Contomastix. The last appears as paraphyletic in a recent phylogenetic reconstruction based on morphology, but monophyletic in a reconstruction using molecular characters. Six species are allocated to Contomastix. One of them, C. lacertoides, having an extensive and disjunct geographic distribution in Argentina, Uruguay and Brazil. Preliminary analyses revealed morphological differences among its populations, suggesting that it is actually a complex of species. Here, we describe a new species corresponding to the Argentinian populations hitherto regarded as C. lacertoides, by integrating morphological and molecular evidence. Furthermore, we demonstrate that the presence of notched proximal margin of the tongue is a character that defines the genus Contomastix.


Phytotaxa ◽  
2018 ◽  
Vol 336 (2) ◽  
pp. 148 ◽  
Author(s):  
CARLOS CERREJÓN ◽  
ENRIQUE MAGUILLA ◽  
DIETMAR QUANDT ◽  
JESÚS MUÑOZ ◽  
MODESTO LUCEÑO

Specimens of Andreaea sect. Andreaea collected in Lesotho show morphological differences from the remaining Sub-Saharan Africa species in the group. Particularly, Lesotho specimens have much larger spores, a character diagnostic in the genus. Spore size also separates the Lesotho specimens from typical A. rupestris from the Northern Hemisphere. Consequently, we describe a new species from the highlands of Lesotho (Andreaea barbarae). Additionally, we present a taxonomic key to all accepted species of Andreaea sect. Andreaea in sub-Saharan Africa.


Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.


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