ÉTUDE DU VIRUS DE L'INFLUENZA: ANTIGÉNICITÉ DE SOUCHES GRIPPALES ADAPTÉES À DES CULTURES DE TISSUS

1961 ◽  
Vol 7 (4) ◽  
pp. 467-472
Author(s):  
A. Boudreault ◽  
V. Pavilanis
Keyword(s):  
Monkey Kidney ◽  
Embryonic Tissue ◽  
Tissue Cultures ◽  
Antigenic Properties ◽  
High Level ◽  
Chick Embryonic Tissue

This paper deals with a study of the antigenic properties of influenza strains adapted to tissue cultures. When adapted to chick embryonic tissue, influenza strains lose most of their antigenicity; when adapted to monkey kidney cultures under the same conditions, influenza strains maintain their antigenic value to a high level.

scholarly journals STUDIES ON THE INACTIVATION OF VACCINE VIRUS AND THE ACTION OF CERTAIN SUBSTANCES UPON THE INFECTING POWER OF THE INACTIVATED VIRUS

1928 ◽  
Vol 47 (3) ◽  
pp. 389-402 ◽  
Author(s):  
F. Duran Reynals
Keyword(s):  
Vaccine Virus ◽  
Embryonic Tissue ◽  
Tissue Cultures ◽  
Supernatant Fluid ◽  
High Susceptibility

Vaccine virus, obtained from testicular inoculation shows a high susceptibility to chloroform as compared with ether, toluene, 95 per cent alcohol and acetone. Vaccine virus, after treatment with an amount of chloroform sufficient to render it incapable or only barely capable of originating an eruption in the rabbit's skin, produces a characteristic eruption when injected with the supernatant fluid of embryonic tissue or sarcoma tissue "cultures" or kieselguhr, substances all of which are markedly irritative to the rabbit's skin. Reactivation of the chloroformed vaccine virus is not possible when chloroform has been added to it in such quantity that the injection of large amounts of the treated virus fails to cause an eruption. Whenever reactivation has been accomplished it has been possible to get a vaccine eruption of greater or less intensity by the injection of large amounts of the chloroformed vaccine alone. Embryo and chicken sarcoma "culture" fluids when injected intradermally make the skin susceptible to the localization of the virus introduced intravenously. The bearing of these experiment on the interpretation of Gye' theory of cancer causation is discussed.

scholarly journals THE ROLE OF METHIONINE DEFICIENCY IN POLIOVIRUS REPLICATION IN TISSUE CULTURES

1966 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Soussan Mohajer ◽  
Janis Gabliks
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Inhibitory Effect ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Experimental Conditions ◽  
Methionine Deficiency ◽  
Intracellular Amino Acids

The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10–3 and 4.0 x 10–3 moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.

scholarly journals PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

1954 ◽  
Vol 99 (2) ◽  
pp. 167-182 ◽  
Author(s):  
R. Dulbecco ◽  
Marguerite Vogt
Keyword(s):  
Virus Particle ◽  
Parent Strain ◽  
High Titer ◽  
Plaque Formation ◽  
Monkey Kidney ◽  
Specific Antiserum ◽  
Tissue Cultures ◽  
Virus Population ◽  
Pure Lines

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 108 plaque-forming particles per ml., were obtained.

Infection of Monkey Kidney Tissue Cultures with Virus-Like Agents.

1955 ◽  
Vol 88 (1) ◽  
pp. 8-16 ◽  
Author(s):  
R. Rustigian ◽  
P. Johnston ◽  
H. Reihart
Keyword(s):  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Cultures

scholarly journals An outbreak of aseptic meningitis associated with a previously unrecognized virus

1961 ◽  
Vol 59 (2) ◽  
pp. 181-189 ◽  
Author(s):  
I. B. R. Duncan
Keyword(s):  
Aseptic Meningitis ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Human Thyroid ◽  
Tissue Cultures ◽  
Human Amnion ◽  
Echo Virus

In 1959, 69 cases of aseptic meningitis were admitted to various hospitals in Scotland—all apparently due to a Hitherto unrecognized virus. This agent had the characteristics of an ECHO virus but differed from the 28 ECHO viruses at present recognized. Seventy-five strains of the virus were isolated, and human thyroid and human amnion tissue cultures proved much superior to monkey kidney tissue cultures for its isolation.

Serial Propagation of Adenoviruses (APG) in Monkey Kidney Tissue Cultures

1956 ◽  
Vol 92 (4) ◽  
pp. 667-669 ◽  
Author(s):  
J. W. Hartley ◽  
R. J. Huebner ◽  
W. P. Rowe
Keyword(s):  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Cultures ◽  
Serial Propagation

Morphogenesis in embryonic tissue cultures of apple

1984 ◽  
Vol 59 (4) ◽  
pp. 469-475 ◽  
Author(s):  
A. C. Rubos ◽  
Janet A. Pryke
Keyword(s):  
Embryonic Tissue ◽  
Tissue Cultures
Sign in / Sign up

Export Citation Format

Share Document