NEW VIRAL AGENTS RECOVERED FROM TISSUE CULTURES OF MONKEY KIDNEY CELLS. II. PROBLEMS OF ISOLATION AND IDENTIFICATION

1957 ◽  
Vol 67 (8) ◽  
pp. 413-423 ◽  
Author(s):  
Robert N. Hull ◽  
James R. Minner
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Isolation And Identification

scholarly journals THE ROLE OF METHIONINE DEFICIENCY IN POLIOVIRUS REPLICATION IN TISSUE CULTURES

1966 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Soussan Mohajer ◽  
Janis Gabliks
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Inhibitory Effect ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Experimental Conditions ◽  
Methionine Deficiency ◽  
Intracellular Amino Acids

The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10–3 and 4.0 x 10–3 moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.

NEW VIRAL AGENTS RECOVERED FROM TISSUE CULTURES OF MONKEY KIDNEY CELLS

1956 ◽  
Vol 63 (2) ◽  
pp. 204-215 ◽  
Author(s):  
ROBERT N. HULL ◽  
JAMES R. MINNER ◽  
JAMES W. SMITH
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

NEW VIRAL AGENTS RECOVERED FROM TISSUE CULTURES OF MONKEY KIDNEY CELLS

1958 ◽  
Vol 68 (1) ◽  
pp. 31-44 ◽  
Author(s):  
ROBERT N. HULL ◽  
JAMES R. MINNER ◽  
CARMINE C. MASCOLI
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

Morphogenesis of a Virus Isolated from a Human with Myelomonocytic Leukemia

1972 ◽  
Vol 30 ◽  
pp. 84-85
Author(s):  
Richard M. Jamison
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Actinomycin D ◽  
Human Cell Line ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Peripheral Leukocytes ◽  
Myelomonocytic Leukemia

A virus has been isolated in tissue culture by co-cultivation in vitro of peripheral leukocytes from a leukemic donor with monkey kidney cells. This virus contains ribonucleic acid, its density is similar to that of members of the oncorna virus group, and its replication is inhibited by Actinomycin D; it is presumably a human oncornavirus. The virus has been detected by electron microscopy both in the peripheral leukocytes of the leukemic donor and in tissue cultures of the continuous monkey kidney cell line originally co-cultivated with these peripheral leukocytes. Plasma from the leukemic donor reacts in indirect immunofluorescence tests with the infected tissue cultures but not with non-infected controls. More recently, infection of a continuous human cell line (FL) has been detected by electron microscopy. Appropriate cell controls are negative.

scholarly journals A COMPARISON OF THE GROWTH OF SELECTED MYCOBACTERIA IN HELA, MONKEY KIDNEY, AND HUMAN AMNION CELLS IN TISSUE CULTURE

1958 ◽  
Vol 107 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Charles C. Shepard
Keyword(s):  
Tissue Culture ◽  
Growth Rates ◽  
Monkey Kidney ◽  
Mycobacterium Fortuitum ◽  
Kidney Cells ◽  
Tubercle Bacillus ◽  
Tissue Cultures ◽  
Pathogenic Species ◽  
Subsequent Growth ◽  
Human Amnion

HeLa, monkey kidney, and human amnion cells in tissue cultures were compared as sites for the multiplication of strains of tubercle bacilli or original and reduced pathogenicity, and for several other species of mycobacteria capable of causing disease in humans. The arrangement of the pathogenic species inorder of their growth rates in HeLa cells was Mycobacterium fortuitum, Mycobacterium balnei, and the "yellow bacillus," followed closely by the tubercle bacillus. This order was also correct for these species in monkey kidney and human amnion cells, and is the same as that seen in bacteriological media. The arrangement of the strains of tubercle bacilli in order of their growth rates in all three types of cells was: H37Rv, then R1Rv, and lastly H37Ra, which multiplied about as slowly as BCG. An INH-resistant strain grew about as rapidly as H37Rv. Growth of the pathogenic species occurred at about the same rates in HeLa and monkey kidney cells, but was distinctly slower in human amnion cells, which are less active metabolically. Irradiation of the cells in doses up to 5000 r did not affect the subsequent growth of mycobacteria in them. Preliminary experiments with human leprosy bacilli indicate that they can be introduced into these cells in high numbers and that the bacilli then persist for the life of the cells.

scholarly journals Expression of porcine pro-opiomelanocortin cDNA in heterologous monkey kidney cells. Biosynthesis and secretion of the prohormone without processing.

1987 ◽  
Vol 262 (4) ◽  
pp. 1876-1881
Author(s):  
G Noël ◽  
L Zollinger ◽  
N Larivière ◽  
C Nault ◽  
P Crine ◽  
...  
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells

scholarly journals Enhanced SV40 Virus Replication in Fully Permissive Monkey Kidney Cells Pre-treated with 5-Iodo-2'-deoxyuridine (IdUrd)

1977 ◽  
Vol 37 (3) ◽  
pp. 569-584 ◽  
Author(s):  
H. G. Suarez ◽  
Ch. Lavialle ◽  
J. Stevenet ◽  
S. Estrade ◽  
A. G. Morris ◽  
...  
Keyword(s):  
Virus Replication ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Sv40 Virus

scholarly journals Enterovirus 70 Receptor Utilization Is Controlled by Capsid Residues That Also Regulate Host Range and Cytopathogenicity

2007 ◽  
Vol 81 (16) ◽  
pp. 8648-8655 ◽  
Author(s):  
Melissa Stewart Kim ◽  
Vincent R. Racaniello
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rhesus Monkey ◽  
Host Range ◽  
Hela Cells ◽  
Lytic Replication ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Cytopathic Effects ◽  
Axis Of Symmetry

ABSTRACT Enterovirus type 70, an etiologic agent of acute hemorrhagic conjunctivitis, may bind different cellular receptors depending on cell type. To understand how EV70-receptor interaction is controlled, we studied two variants of the virus with distinct receptor utilization. EV70-Rmk, derived by passage in rhesus monkey kidney cells, replicates poorly in HeLa cells and does not cause cytopathic effects. Decay accelerating factor (DAF) is not a cell receptor for EV70-Rmk. Passage of EV70-Rmk in HeLa cells lead to isolation of EV70-Dne, which does not replicate in rhesus monkey kidney cells but grows to high titers in HeLa cells and causes cytopathic effects. DAF is sufficient for cell entry of EV70-Dne. EV70-Rmk replicates in human eye and brain-derived cell lines, whereas the Dne strain replicates only in HeLa cells and in conjunctiva-derived 15C4 cells. The two EV70 strains differ by five amino acid changes in the viral capsid. Single substitution of four of the five EV70-Rmk amino acids with the residue from EV70-Dne leads to lytic replication in HeLa cells. Conversely, substitution of any of the five EV70-Dne amino acids with the EV70-Rmk amino acid does not alter replication in HeLa cells. Three of these capsid amino acids are predicted to be located in the canyon encircling the fivefold axis of symmetry, one amino acid is found at the fivefold axis of symmetry, and one is located the interior of the capsid. The five EV70 residues define a region of the capsid that controls viral host range, DAF utilization, and cytopathogenicity.

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