General characteristics and viral susceptibility of bovine embryonic tissue cultures

Virology ◽  
1957 ◽  
Vol 4 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Joel Warren ◽  
Ernest C. Cutchins
Keyword(s):  
Embryonic Tissue ◽  
Tissue Cultures ◽  
Viral Susceptibility

Viral Susceptibility and Embryonic Differentiation.

Development ◽  
1963 ◽  
Vol 11 (4) ◽  
pp. 757-764
Author(s):  
Juhani Rapola ◽  
Tapani Vainio ◽  
Lauri Saxén
Keyword(s):  
Tissue Culture ◽  
Viral Replication ◽  
Metabolic Rate ◽  
Tissue Damage ◽  
Embryonic Tissue ◽  
High Metabolic Rate ◽  
Viral Susceptibility ◽  
Proliferative Rate ◽  
Active Proliferation ◽  
Severe Tissue

The fact that viral susceptibility changes during embryogenesis has been pointed out by both experimental embryologists and clinical practitioners, not to mention virologists working with avian material. In attempts to find the fundamental factors which make embryonic tissue susceptible or resistant to a given virus, the metabolic and proliferative rate have been considered relevant (Williamson et al., 1953; Robertson et al., 1955; Töndury, 1956). Experience accumulated in studies of the replication of various viruses in tissue culture has taught us that a high metabolic rate and active proliferation may not always enhance viral replication (Ginsberg, 1958). However, there seems to be justification for the view that an injurious agent leads to more severe tissue damage when it exercises its effect upon actively proliferating tissues than when it does so at the ‘resting stage’.

scholarly journals STUDIES ON THE INACTIVATION OF VACCINE VIRUS AND THE ACTION OF CERTAIN SUBSTANCES UPON THE INFECTING POWER OF THE INACTIVATED VIRUS

1928 ◽  
Vol 47 (3) ◽  
pp. 389-402 ◽  
Author(s):  
F. Duran Reynals
Keyword(s):  
Vaccine Virus ◽  
Embryonic Tissue ◽  
Tissue Cultures ◽  
Supernatant Fluid ◽  
High Susceptibility

Vaccine virus, obtained from testicular inoculation shows a high susceptibility to chloroform as compared with ether, toluene, 95 per cent alcohol and acetone. Vaccine virus, after treatment with an amount of chloroform sufficient to render it incapable or only barely capable of originating an eruption in the rabbit's skin, produces a characteristic eruption when injected with the supernatant fluid of embryonic tissue or sarcoma tissue "cultures" or kieselguhr, substances all of which are markedly irritative to the rabbit's skin. Reactivation of the chloroformed vaccine virus is not possible when chloroform has been added to it in such quantity that the injection of large amounts of the treated virus fails to cause an eruption. Whenever reactivation has been accomplished it has been possible to get a vaccine eruption of greater or less intensity by the injection of large amounts of the chloroformed vaccine alone. Embryo and chicken sarcoma "culture" fluids when injected intradermally make the skin susceptible to the localization of the virus introduced intravenously. The bearing of these experiment on the interpretation of Gye' theory of cancer causation is discussed.

Morphogenesis in embryonic tissue cultures of apple

1984 ◽  
Vol 59 (4) ◽  
pp. 469-475 ◽  
Author(s):  
A. C. Rubos ◽  
Janet A. Pryke
Keyword(s):  
Embryonic Tissue ◽  
Tissue Cultures

In vitro cultivation of Choristoneura fumiferana (Clemens) (Lepidoptera:Tortricidae) tissues

1968 ◽  
Vol 46 (1) ◽  
pp. 11-13 ◽  
Author(s):  
S. S. Sohi
Keyword(s):  
Choristoneura Fumiferana ◽  
Ovarian Tissue ◽  
Good Condition ◽  
Spruce Budworm ◽  
Embryonic Tissue ◽  
In Vitro Cultivation ◽  
Tissue Cultures ◽  
Cell Sheets ◽  
Free Cells

Embryonic tissue fragments of the spruce budworm, Choristoneura fumiferana (Clemens), survived in vitro for 6–7 weeks. Three- to five-day-old eggs, maintained at 22 °C, were used to obtain embryos which were cut into small pieces and maintained in drop cultures using Grace's medium. Explants contracted rhythmically for 3–4 weeks and stayed in good condition for a further 2–3 weeks. Explanted tissues produced three types of growth: hollow vesicles, continuous cell sheets, and free cells. The size of vesicles in some cases was 4–5 times that of the explant. The free cells increased in number considerably and formed good monolayers. The cultures deteriorated after 6–7 weeks. Ovarian tissue cultures of late sixth-instar larvae of this insect did not produce any growth, and deteriorated in 3–4 days.

ÉTUDE DU VIRUS DE L'INFLUENZA: ANTIGÉNICITÉ DE SOUCHES GRIPPALES ADAPTÉES À DES CULTURES DE TISSUS

1961 ◽  
Vol 7 (4) ◽  
pp. 467-472
Author(s):  
A. Boudreault ◽  
V. Pavilanis
Keyword(s):  
Monkey Kidney ◽  
Embryonic Tissue ◽  
Tissue Cultures ◽  
Antigenic Properties ◽  
High Level ◽  
Chick Embryonic Tissue

This paper deals with a study of the antigenic properties of influenza strains adapted to tissue cultures. When adapted to chick embryonic tissue, influenza strains lose most of their antigenicity; when adapted to monkey kidney cultures under the same conditions, influenza strains maintain their antigenic value to a high level.

Comparison of Choriocarcinoma and Normal Placenta

1971 ◽  
Vol 29 ◽  
pp. 324-325
Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord
Keyword(s):  
Cell Lines ◽  
Small Cells ◽  
Tissue Cultures ◽  
Glycogen Depletion ◽  
Cell Surfaces ◽  
Intercellular Spaces ◽  
Concomitant Increase ◽  
Gestational Choriocarcinoma ◽  
Cytoplasmic Glycogen ◽  
Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

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