Further studies on the differentiation of Clostridium sordellii from Clostridium bifermentans by gas chromatography

1970 ◽  
Vol 16 (11) ◽  
pp. 1071-1078 ◽  
Author(s):  
John B. Brooks ◽  
V. R. Dowell ◽  
D. C. Farshy ◽  
A. Y. Armfield
Keyword(s):  
Gas Chromatography ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Carbohydrate Composition ◽  
Whole Cells ◽  
Cultural Medium ◽  
Urease Production ◽  
Clostridium Bifermentans ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Amines produced by 31 strains of the Clostridium bifermentans and C. sordellii groups were compared by examining trifluoroaceticanhydride derivatives of basic chloroform extracts from spent cultural medium by gas–liquid chromatography (g.l.c.). All of the urease-positive strains (16) exhibited an amine profile consistent with that of C. sordellii. On the other hand, 12 of 15 urease-negative strains produced amine g.l.c. patterns like that of C. bifermentans, and three strains produced amine patterns identical with that of C. sordellii. The carbohydrate composition of some of the strains was determined by g.l.c. of trimethylsilyl derivatives of acid-digested formamide extracts of whole cells. Two of the three urease-negative strains with amine profiles like C. sordellii had a carbohydrate composition similar to that of C. sordellii, and the other strain had a carbohydrate profile more like that of C. bifermentans. One known strain of C. bifermentans had a carbohydrate profile with characteristics of both C. bifermentans and C. sordellii. The results of this study point out the variability of urease production by C. sordellii and the value of gas chromatography in differentiating this organism from C. bifermentans.

scholarly journals Characterization and identification of 95 diphtheroid (group JK) cultures isolated from clinical specimens

1979 ◽  
Vol 9 (3) ◽  
pp. 418-424 ◽  
Author(s):  
P S Riley ◽  
D G Hollis ◽  
G B Utter ◽  
R E Weaver ◽  
C N Baker
Keyword(s):  
Liquid Chromatography ◽  
Antimicrobial Agents ◽  
Blood Cultures ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Clinical Specimens ◽  
Bacterial Cultures ◽  
Carbohydrate Fermentation ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Ninety-five cultures of group JK bacteria isolated from clinical specimens were characterized morphologically and biochemically. The microorganisms were isolated primarily from blood cultures. The bacterial cultures produced positive reactions when tested for catalase, Tween hydrolysis, and carbohydrate fermentation. Glucose and galactose were fermented by more than 90% of the organisms. Gas-liquid chromatography of trimethylsilyl derivatives of whole-cell hydrolysates of some of the group JK cultures yielded nearly identical elution profiles. The group JK microorganisms were susceptible to vancomycin but were resistant to most of the other 17 antimicrobial agents tested. A method is presented for differentiating the group JK microorganisms from other similar bacteria encountered in clinical specimens. Although these bacteria rarely occur in clinical specimens, they are capable of producing fatal infections (endocarditis and sepsis) in humans.

Gas Chromatography of the Trimethylsilyl Derivatives of Flavanones

1971 ◽  
Vol 54 (5) ◽  
pp. 1211-1214 ◽  
Author(s):  
D E Coffin ◽  
J E Dupont
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Room Temperature ◽  
Gas Liquid Chromatography ◽  
Hydrogen Flame ◽  
Flame Detection ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Abstract A procedure was developed for the detection and measurement by gas-liquid chromatography of the 4 flavanones isosakuranetin, naringenin, hesperetin, and eriodictyol as their trimethylsilyl derivatives. The derivatives were prepared by treatment of the flavanones with N,0-bis(trimethylsilyl) acetamide in N,N-dimethylformamide at room temperature. The derivatives of naringenin and hesperetin were separated, detected, and measured by gas-liquid chromatography on a column of OV-101 on Diatoport S with hydrogen flame detection. Isosakuranetin and eriodictyol derivatives could be detected but were not adequately separated by this system. These procedures were applied to the detection of the flavanones derived from orange and grapefruit juices.

scholarly journals Gas-Chromatographic Determination of the Trimethylsilyl Derivatives of Polyhydric Alcohol Humectants in Tobacco and in Tobacco Smoke

1971 ◽  
Vol 6 (2) ◽  
Author(s):  
N. Carugno ◽  
S. Rossi ◽  
G. Lionetti
Keyword(s):  
Gas Chromatography ◽  
Mass Spectra ◽  
Triethylene Glycol ◽  
Chromatographic Determination ◽  
Flame Ionization ◽  
Trimethylsilyl Derivatives ◽  
Gas Chromatographic Method ◽  
Derivatives Of ◽  
Quantitative Procedure

AbstractA qualitative and quantitative procedure has been developed for the determination of humectants in manufactured tobacco by gas-chromatographic method with a flame ionization detector. It consists of extraction with methanol, concentration of the extract and treatment with Tri-Sil reagents. The operating gas-chromatographic conditions are set forth. Samples of tobacco containing glycerine, propylene glycol, diethylene glycol, triethylene glycol, 1-3 butylene glycol and sorbitol have been analysed with recoveries, for the first five ones, in the range of 95-104 %. In order to verify that each chromatographic peak corresponded to the relative glycol, with no interference by other silylated compounds, the mass spectra were obtained through the combination of gas-chromatography with mass spectrometry. The results achieved confirm, as far as tobacco is concerned, that the procedure is accurate and precise. The same method for the determination of humectants was extended to cigarette smoke. Even though this involves morecomplicated problems, as compared to tobacco, because of the presence of silylated compounds, it was found that, for certain glycols, the gas-chromatography of the trimethyl derivatives can be also used as a method of analysis. The mass spectra of some polyhydric alcohols are shown

Gas chromatography and mass spectrometry of some trimethylsilyl derivatives of urinary glucuronides

1974 ◽  
Vol 57 (1) ◽  
pp. 173-189 ◽  
Author(s):  
J.E. Mrochek ◽  
W.T. Rainey
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

scholarly journals Gas Chromatography of Trifluoroacetyl Derivatives of Alditols and Trimethylsilyl Derivatives of Aldonolactones

1968 ◽  
Vol 16 (7) ◽  
pp. 1383-1387 ◽  
Author(s):  
MICHIO MATSUI ◽  
MASASHI OKADA ◽  
TOSHIO IMANARI ◽  
ZENZO TAMURA
Keyword(s):  
Gas Chromatography ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Preparation of trimethylsilyl derivatives of ribonucleosides for gas chromatography

1985 ◽  
Vol 348 ◽  
pp. 280-285 ◽  
Author(s):  
E. Csató ◽  
P. Keresztes ◽  
L.D. Szabó ◽  
D. Knausz
Keyword(s):  
Gas Chromatography ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Gas Chromatography of Trimethylsilyl Derivatives of Compounds Related to Chloramphenicol.

1963 ◽  
Vol 35 (11) ◽  
pp. 1580-1582 ◽  
Author(s):  
P. D. Shaw
Keyword(s):  
Gas Chromatography ◽  
Trimethylsilyl Derivatives ◽  
Derivatives Of

Differentiation of Actinomyces propionicus from Actinomyces israelii and Actinomyces naeslundii by gas chromatography

1968 ◽  
Vol 14 (7) ◽  
pp. 749-753 ◽  
Author(s):  
Yu-Ying F. Li ◽  
Lucille K. Georg
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Actinomyces Naeslundii ◽  
Actinomyces Species ◽  
End Products

Gas–liquid chromatography (g.l.c.) was used for the analysis of certain metabolic end products of Actinomyces propionicus, as an aid in the separation of this organism from the morphologically similar Actinomyces species, A. israelii and A. naeslundii. Profiles of the chromatograms for the major volatile acids of five strains of A. propionicus studied were found to be distinct from those of four strains of A. israelii and four strains of A. naeslundii. The ratio of propionic acid to acetic acid was approximately 50 times as great for A. propionicus as for the other Actinomyces species. Formic acid was present in significant amounts in both A. israelii and A. naeslundii, but was present only in trace amounts in A. propionicus.Two major nonvolatile acids, lactic and succinic, were identified for the A. israelii and A. naeslundii strains. One of the A. propionicus strains also showed both acids in significant amounts; however, the other four strains of A. propionicus showed succinic acid in large amounts, but only trace amounts of lactic acid.

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