Adenovirus serum neutralization in micro-tissue cultures of rhesus monkey kidney cells

1972 ◽  
Vol 18 (10) ◽  
pp. 1603-1606
Author(s):  
J. Furesz ◽  
R. E. Armstrong ◽  
F. P. Nagler
Keyword(s):  
Rhesus Monkey ◽  
Serum Antibody ◽  
Human Adenovirus ◽  
Neutralization Assay ◽  
Monkey Kidney ◽  
Virus Infectivity ◽  
Kidney Cells ◽  
Test Results ◽  
Antibody Titers ◽  
Passage Cells

A micro-tissue culture assay using 2nd passage cells of primary rhesus monkey kidney for the neutralization of human adenovirus types is described. All 31 serotypes of adenovirus showed characteristic cytopathic changes 3 to 4 days after initiation of the test, and serum antibody titers were established on the 5th day. Virus infectivity and serum antibody titers were found to be greater than in the conventional tube test. Replicate neutralization test results demonstrated the good reproducibility of the micro assay. Heterotypic neutralization tests were carried out on six representative equine reference sera with all prototype viruses, and the specificity of the micro-assay system was established. Preliminary experiments indicated that 2nd passage Cercopithecus monkey kidney cells were also suitable for the micro-neutralization assay.

scholarly journals Enterovirus 70 Receptor Utilization Is Controlled by Capsid Residues That Also Regulate Host Range and Cytopathogenicity

2007 ◽  
Vol 81 (16) ◽  
pp. 8648-8655 ◽  
Author(s):  
Melissa Stewart Kim ◽  
Vincent R. Racaniello
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rhesus Monkey ◽  
Host Range ◽  
Hela Cells ◽  
Lytic Replication ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Cytopathic Effects ◽  
Axis Of Symmetry

ABSTRACT Enterovirus type 70, an etiologic agent of acute hemorrhagic conjunctivitis, may bind different cellular receptors depending on cell type. To understand how EV70-receptor interaction is controlled, we studied two variants of the virus with distinct receptor utilization. EV70-Rmk, derived by passage in rhesus monkey kidney cells, replicates poorly in HeLa cells and does not cause cytopathic effects. Decay accelerating factor (DAF) is not a cell receptor for EV70-Rmk. Passage of EV70-Rmk in HeLa cells lead to isolation of EV70-Dne, which does not replicate in rhesus monkey kidney cells but grows to high titers in HeLa cells and causes cytopathic effects. DAF is sufficient for cell entry of EV70-Dne. EV70-Rmk replicates in human eye and brain-derived cell lines, whereas the Dne strain replicates only in HeLa cells and in conjunctiva-derived 15C4 cells. The two EV70 strains differ by five amino acid changes in the viral capsid. Single substitution of four of the five EV70-Rmk amino acids with the residue from EV70-Dne leads to lytic replication in HeLa cells. Conversely, substitution of any of the five EV70-Dne amino acids with the EV70-Rmk amino acid does not alter replication in HeLa cells. Three of these capsid amino acids are predicted to be located in the canyon encircling the fivefold axis of symmetry, one amino acid is found at the fivefold axis of symmetry, and one is located the interior of the capsid. The five EV70 residues define a region of the capsid that controls viral host range, DAF utilization, and cytopathogenicity.

Primary isolation of cytopathic bovine rotaviruses on fetal rhesus monkey kidney cells

1983 ◽  
Vol 8 (2) ◽  
pp. 135-139 ◽  
Author(s):  
Yosuke Murakami ◽  
Nobuyoshi Nishioka ◽  
Yuji Hashiguchi ◽  
Chikara Kuniyasu
Keyword(s):  
Rhesus Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Primary Isolation ◽  
Fetal Rhesus Monkey Kidney

Evidence for a bovine origin of the polyomaviurs detected in foetal rhesus monkey kidney cells, FRhK-4 and -6

1983 ◽  
Vol 78 (3-4) ◽  
pp. 151-165 ◽  
Author(s):  
J. V. Parry ◽  
M. H. Lucas ◽  
J. E. Richmond ◽  
S. D. Gardner
Keyword(s):  
Rhesus Monkey ◽  
Monkey Kidney ◽  
Kidney Cells

Non-Specific Hemadsorption by Rhesus Monkey Kidney Cells

1966 ◽  
Vol 121 (1) ◽  
pp. 193-198 ◽  
Author(s):  
W. R. Dowdle ◽  
R. Q. Robinson
Keyword(s):  
Rhesus Monkey ◽  
Monkey Kidney ◽  
Kidney Cells

Variola Virus and Immunity in Smallpox. H, Recovery of Variola-Specific Soluble Antigens from Infected Rhesus Monkey Kidney Cells

1971 ◽  
Vol 123 (4) ◽  
pp. 399-405 ◽  
Author(s):  
R. L. Anthony ◽  
R. W. Daniel ◽  
J. L. Cole ◽  
G. G. Heiner ◽  
F. R. McCrumb
Keyword(s):  
Rhesus Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Variola Virus

scholarly journals Clinical features of cats diagnosed with coccidioidomycosis in Arizona, 2004–2018

2019 ◽  
Vol 22 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Nichole Arbona ◽  
Christine D Butkiewicz ◽  
Minta Keyes ◽  
Lisa F Shubitz
Keyword(s):  
Tertiary Care ◽  
Serum Antibody ◽  
Clinical Signs ◽  
Respiratory Illness ◽  
Test Results ◽  
Oral Fluconazole ◽  
Cell Counts ◽  
Antibody Titers ◽  
Additional Therapy ◽  
Antibody Tests

Objectives The goal of this study was to describe the clinical presentation, diagnosis and treatment of coccidioidomycosis in cats residing in a region endemic for Coccidioides species. Methods A retrospective review of records was performed at both primary and tertiary care veterinary practices in Tucson and Phoenix, Arizona, USA. Data collected included signalment, clinical signs, physical examination findings, diagnostic test results, treatment and outcome. Results Fifty-one feline cases were identified from six veterinary hospitals. Cats presented with clinical signs and laboratory abnormalities similar to what has been seen in dogs, including respiratory illness (n = 20/51), neutrophilia (n = 24/31), monocytosis (n = 17/31) and hyperglobulinemia (n = 16/30). However, cats at diagnosis were typically significantly ill, with 31/51 having disseminated infection, most commonly to the skin (n = 22). Additionally, 43/44 cats that had serum antibody tests performed were positive, and median titer at diagnosis was 1:32 (range 1:4 to ⩾1:256). Serum antibody titers were significantly reduced ( P ⩽0.001) in cats that responded to treatment compared with cats that did not clinically improve. Forty of 46 cats that were treated with oral fluconazole responded and did not require additional therapy. Fourteen cats developed recurrent disease and all but one had antifungal therapy successfully reinstituted. Conclusions and relevance Coccidioidomycosis is a disease of concern for cats residing in the regions endemic for Coccidioides species. Disease is most often disseminated at the time of diagnosis, possibly due to delays in presentation for care and recognition of the infection. Suspicion of disease, serum chemistries, blood cell counts, presence of antibody and imaging aid in the diagnosis of coccidioidomycosis in cats. Serum antibody reduction during treatment frequently correlated with an adequate response to medication. Consideration of coccidioidomycosis as a cause of illness will lead to earlier diagnosis and potentially better treatment outcomes in cats.

scholarly journals Epidemiology of Commercial Rhesus Monkey Kidney Cells Contaminated with Coccidioides posadasii

2013 ◽  
Vol 51 (6) ◽  
pp. 2005-2005 ◽  
Author(s):  
A. Purfield ◽  
N. Ahmad ◽  
B. J. Park ◽  
D. Kuhles ◽  
K. St. George ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Coccidioides Posadasii
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