Inability to rescue viral genes from human cells biochemically transformed by herpes simplex virus type 2 DNA

Thymidine kinase negative (TK−) mutants of HSV-1 were used to attempt to rescue HSV-2 genes from cells biochemically transformed with HSV-2 DNA. The results indicate that the occurrence of a rescue event was less than 7 × 10−7 in this system.

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Phosphorylation of four acyclic guanosine analogs by herpes simplex virus type 2 thymidine kinase.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.25.4.524 ◽

1984 ◽

Vol 25 (4) ◽

pp. 524-526 ◽

Cited By ~ 8

Author(s):

A Larsson ◽

P Z Tao

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Thymidine Kinase ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Simplex Virus

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Spontaneous reactivation of acyclovir resistant (ACVr) thymidine kinase deficient (T K−) herpes simplex virus type 2 (HSV 2) is still dependent upon viral TK expression

Antiviral Research ◽

10.1016/0166-3542(95)94890-e ◽

1995 ◽

Vol 26 (3) ◽

pp. A327

Author(s):

J.J. Sasadeusz ◽

S. Safrin ◽

S.L. Sacks

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Thymidine Kinase ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Simplex Virus ◽

Spontaneous Reactivation

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Peptide sequences of glycoprotein G-2 discriminate between herpes simplex virus type 2 (HSV-2) and HSV-1 antibodies.

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.3.3.265-269.1996 ◽

1996 ◽

Vol 3 (3) ◽

pp. 265-269 ◽

Cited By ~ 17

Author(s):

M Levi ◽

U Rudén ◽

B Wahren

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Glycoprotein G ◽

Simplex Virus ◽

Hsv 1

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Lasar flow cytophotometric immunoperoxidase detection of herpes simplex virus type 2 antigens in infected cultured human cells.

Journal of Histochemistry & Cytochemistry ◽

10.1177/24.12.187690 ◽

1976 ◽

Vol 24 (12) ◽

pp. 1249-1257 ◽

Cited By ~ 6

Author(s):

J F Leary ◽

M F Notter ◽

P Todd

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Large Angle ◽

Human Cells ◽

Staining Reaction ◽

Infected Cells ◽

Simplex Virus

Human cells in culture (HEp-2) were infected with herpes simplex virus type 2 (HSV-2) at multiplicities of infection varying from 0.2 to 10, and fixed 6, 12, 18 and 24 hr after infection. Infection-related antigens were detected by an indirect double antibody (peroxidase conjugated goat anti-rabbit to rabbit anti-herpes simplex virus type 2) immunoenzymatic staining reaction that rendered infection-related antigens visible by light microscopy. A corresponding series of laser flow cytophotometric experiments yielded reproducible large-angle (1-19 degrees) laser-light scattering distributions that depended upon multiplicities of infection and the location of the infection-related antigens in the infected cells.

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CD4+ T-cell responses to herpes simplex virus type 2 (HSV-2) glycoprotein G are type specific and differ in symptomatic and asymptomatic HSV-2-infected individuals

Journal of General Virology ◽

10.1099/vir.0.79978-0 ◽

2004 ◽

Vol 85 (8) ◽

pp. 2139-2147 ◽

Cited By ~ 25

Author(s):

Kristina Eriksson ◽

Lars Bellner ◽

Staffan Görander ◽

Gun-Britt Löwhagen ◽

Petra Tunbäck ◽

...

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

T Cell ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Mononuclear Cells ◽

Glycoprotein G ◽

Simplex Virus ◽

Hsv 1

T-cell recognition of the secreted and membrane-bound portions of the herpes simplex virus type 2 (HSV-2) glycoprotein G (sgG-2 and mgG-2, respectively) was compared in symptomatic and asymptomatic HSV-2-infected individuals and in HSV-2-seronegative controls and the responses with HSV-1 glycoproteins C and E (gC-1 and gE-1) were compared. CD4+ T cells from HSV-2-infected individuals specifically recognized both sgG-2 and mgG-2, whereas HSV-1-infected and HSV-seronegative controls did not respond to these glycoproteins. The responses to gC-1 and gE-1, on the other hand, were not type specific, as blood mononuclear cells from both HSV-1- and HSV-2-infected individuals responded in vitro. There was an association between the status of the infection (symptomatic versus asymptomatic) and the CD4+ T-cell responsiveness. Symptomatic HSV-2-seropositive individuals responded with significantly lower Th1 cytokine production to sgG-2 and mgG-2 than did asymptomatic HSV-2-infected carriers, especially within the HSV-1-negative cohort. No differences in T-cell proliferation were observed between asymptomatic and symptomatic individuals. The results have implications for studies of HSV-2-specific CD4+ T-cell reactivity in general and for analysis of immunological differences between asymptomatic and symptomatic individuals in particular.

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