Induction of apoptosis in HT29 human intestinal epithelial cells by the cytotoxic enterotoxin ofAeromonas hydrophila

2001 ◽  
Vol 79 (4) ◽  
pp. 525-531 ◽  
Author(s):  
Rosabel M Falcón ◽  
Hernandes F Carvalho ◽  
Paulo Pinto Joazeiro ◽  
Maria S. Viccari Gatti ◽  
Tomomasa Yano
Keyword(s):  
Aeromonas Hydrophila ◽  
Gel Electrophoresis ◽  
Light And Electron Microscopy ◽  
Intestinal Cells ◽  
Agarose Gel ◽  
Intestinal Epithelial ◽  
Agarose Gel Electrophoresis ◽  
Nuclear Condensation ◽  
Gastrointestinal Infections ◽  
Human Intestinal Cells

The cytotoxic enterotoxin produced by Aeromonas hydrophila is considered to be the main virulence factor in gastrointestinal infections mediated by this pathogen. In this study, we examined the morphological and apoptotic effects of this toxin on HT29 cells, using light and electron microscopy in situ, as well as agarose gel electrophoresis of cell DNA. Cells treated with the cytotoxic enterotoxin became round and lost their polarity as well as their adhesion to each other and to the substrate. Cytoplasmic blebbing and nuclear condensation also occurred. DNA fragmentation was detected by TUNEL labelling and agarose gel electrophoresis. These results show that the cytotoxic enterotoxin of A. hydrophila can induce apoptosis in human intestinal cells in culture.Key words: Aeromonas hydrophila, cytotoxic enterotoxin, aerolysin, apoptosis, human intestinal cells.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

Analysis of simian virus 40 wild-type and mutant virions by agarose gel electrophoresis.

1976 ◽  
Vol 17 (3) ◽  
pp. 916-923 ◽  
Author(s):  
M Zweig ◽  
S Barban ◽  
N P Salzman
Keyword(s):  
Gel Electrophoresis ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Wild Type

scholarly journals Studies of Transferrin Polymorphism in Swedish Cattle Using Agarose Gel Electrophoresis

1976 ◽  
Vol 17 (4) ◽  
pp. 451-457
Author(s):  
Per Liberg ◽  
Gunnar Carlström
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Transferrin Polymorphism

Multiplasmid DNA in agarose gel electrophoresis: Suitable dye and temperature is essential for prominent profiles

2021 ◽  
Author(s):  
Qiuxia Jiao ◽  
Zhao Xin ◽  
Qiufeng Gou ◽  
Kang Cao ◽  
Qu Pan
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

scholarly journals Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Claudia Gili ◽  
Federico Bonsembiante ◽  
Renzo Bonanni ◽  
Alessia Giordano ◽  
Sabina Ledda ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Tursiops Truncatus ◽  
Bottlenose Dolphins ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Capillary Zone
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