Gene for integrin-associated protein (IAP, CD47): Physical mapping, genomic structure, and expression studies in skeletal muscle

2002 ◽  
Vol 80 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Jörg Schickel ◽  
Katharina Stahn ◽  
Klaus-Peter Zimmer ◽  
Ralf Sudbrak ◽  
Tim M Størm ◽  
...  

Integrin-associated protein (IAP) is a widely expressed membrane protein with multiple functions in immunological and neuronal processes. Having physically mapped the IAP gene into a BAC/PAC contig covering approximately 1 Mb on human chromosome 3q13.1-q13.2, we determined the genomic organization of the gene, established its expression in skeletal muscle, and identified a novel splice variant. Our expression studies demonstrate expression of integrin-associated protein in the t-tubular system and the euchromatin of skeletal muscle cells where its function thus far is not known.Key words: integrin-associated protein, novel splice form, expression and subcellular localization in skeletal muscle, genomic organization.

1992 ◽  
Vol 1 (7) ◽  
pp. 521-527 ◽  
Author(s):  
Andrea I.McClatchey ◽  
Carol S.Lin ◽  
Jianzhou Wang ◽  
Eric P.Hoffman ◽  
Cecilia Rojas ◽  
...  

Genomics ◽  
1997 ◽  
Vol 42 (2) ◽  
pp. 284-294 ◽  
Author(s):  
M.A. Nesbit ◽  
M.D. Hodges ◽  
L. Campbell ◽  
T.M.A.M.O. de Meulemeester ◽  
M. Alders ◽  
...  

1972 ◽  
Vol 59 (3) ◽  
pp. 347-359 ◽  
Author(s):  
P. C. Vaughan ◽  
J. N. Howell ◽  
R. S. Eisenberg

The capacitance of skeletal muscle fibers was measured by recording with one microelectrode the voltage produced by a rectangular pulse of current applied with another microelectrode. The ionic strength of the bathing solution was varied by isosmotic replacement of NaCl with sucrose, the [K] [Cl] product being held constant. The capacitance decreased with decreasing ionic strength, reaching a value of some 2 µF/cm2 in solutions of 30 mM ionic strength, and not decreasing further in solutions of 15 mM ionic strength. The capacitance of glycerol-treated fibers did not change with ionic strength and was also some 2 µF/cm2. It seems likely that lowering the ionic strength reduces the capacitance of the tubular system (defined as the charge stored in the tubular system), and that the 2 µF/cm2 which is insensitive to ionic strength is associated with the surface membrane. The tubular system is open to the external solution in low ionic strength solutions since peroxidase is able to diffuse into the lumen of the tubules. Twitches and action potentials were also recorded from fibers in low ionic strength solutions, even though the capacitance of the tubular system was very small in these solutions. This finding can be explained if there is an action potential—like mechanism in the tubular membrane.


Gene ◽  
2004 ◽  
Vol 338 (2) ◽  
pp. 243-256 ◽  
Author(s):  
Chinami Hirao ◽  
Izuru Yonemura ◽  
Jun-Ichi Miyazaki

1992 ◽  
Vol 182 (2) ◽  
pp. 794-801 ◽  
Author(s):  
Jianzhou Wang ◽  
Cecilia V. Rojas ◽  
Jianhua Zhou ◽  
Lisa S. Schwartz ◽  
Hugh Nicholas ◽  
...  

2001 ◽  
Vol 10 (2) ◽  
pp. 90-94 ◽  
Author(s):  
J. Frank ◽  
P. B. Cserhalmi-Friedman ◽  
W. Ahmad ◽  
A. A. Panteleyev ◽  
V. M. Aita ◽  
...  

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