The Site of Protein Synthesis in the Moss Tortula ruralis on Recovery from Desiccation

1974 ◽  
Vol 52 (4) ◽  
pp. 345-348 ◽  
Author(s):  
Edward B. Tucker ◽  
J. Derek Bewley
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Electrophoretic Mobility ◽  
Wheat Germ ◽  
Tortula Ruralis ◽  
Cytoplasmic Type ◽  
Sedimentation Value ◽  
In Vivo Protein Synthesis

The major ribosomal species in the rehydrated moss Tortula ruralis had a sedimentation value of 80 S. The electrophoretic mobility of the major rRNA from T. ruralis was the same as the mobility of rRNA from wheat germ, but was different from the mobility of rRNA from Escherichia coli. In vivo protein synthesis upon rehydration of the dehydrated moss was inhibited 80% in the presence of cycloheximide. Upon rehydration of dehydrated T. ruralis it appears that the polyribosomes that are present are composed of cytoplasmic-type ribosomes.

scholarly journals LOCALIZED MUTAGENESIS FOR THE ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF ESCHERICHIA COLI AFFECTED IN PROTEIN SYNTHESIS

Genetics ◽  
1979 ◽  
Vol 91 (2) ◽  
pp. 215-227
Author(s):  
W Scott Champney
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Temperature Sensitive ◽  
Chromosomal Locus ◽  
Ribosomal Subunits ◽  
Prolonged Incubation ◽  
Temperature Sensitive Mutants ◽  
Inhibition Of Growth ◽  
Localized Mutagenesis

ABSTRACT Two variations of the method of localized mutagenesis were used to introduce mutations into the 72 min region of the Escherichia coli chromosome. Twenty temperature-sensitive mutants, with linkage to markers in this region, have been examined. Each strain showed an inhibition of growth in liquid medium at 44°, and 19 of the mutants lost viability upon prolonged incubation at this temperature. A reduction in the rate of in vivo RNA and protein synthesis was observed for each mutant at 44°, relative to a control strain. Eleven of the mutants were altered in growth sensitivity or resistance to one or more of three ribosomal antibiotics. The incomplete assembly of ribosomal subunits was detected in nine strains grown at 44°. The characteristics of these mutants suggest that many of them are altered in genes for translational or transcriptional components, consistent with the clustering of these genes at this chromosomal locus.

Determination of in vivo protein synthesis in human T lymphocytes

2001 ◽  
Vol 20 (2) ◽  
pp. 181-182 ◽  
Author(s):  
A. JANUSZKIEWICZ ◽  
P. ESSÉN ◽  
M.A. McNURLAN ◽  
O. RINGDÉN ◽  
P.J. GARLICK ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
T Lymphocytes ◽  
Human T Lymphocytes ◽  
In Vivo Protein Synthesis

Ricin inhibition of in vivo protein-synthesis in castor beans

1984 ◽  
Vol 36 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Suzanne M. Harley ◽  
Harry Beevers
Keyword(s):  
Protein Synthesis ◽  
In Vivo Protein Synthesis

Initiation of in vivo protein synthesis with non-methionine amino acids

Biochemistry ◽  
1990 ◽  
Vol 29 (18) ◽  
pp. 4263-4268 ◽  
Author(s):  
Ranjan Chattapadhyay ◽  
Heike Pelka ◽  
LaDonne H. Schulman
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
In Vivo Protein Synthesis
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