Nature of lysozyme–water interactions by proton NMR

1991 ◽  
Vol 69 (5-6) ◽  
pp. 341-345 ◽  
Author(s):  
S. Prosser ◽  
H. Peemoeller
Keyword(s):  
Relaxation Rate ◽  
Proton Nmr ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Spin Lattice Relaxation ◽  
Water Proton ◽  
Proton Relaxation ◽  
Spin Lattice ◽  
Proton Coupling ◽  
Relaxation Measurements

Proton spin-lattice relaxation measurements were performed in 10 mM lysozyme solution as a function of temperature and degree of substitution of solvent H2O with D2O. The results show that in the temperature range from 274 to 323 K, the intermolecular lysozyme proton water proton coupling contributes appreciably to the observed water proton relaxation rate. In this system exchange between water protons and labile protein protons does not dominate the behaviour with temperature of the water–lysozyme intermolecular contribution to the spin-lattice relaxation.Key words: NMR, lysozyme, relaxation-contributions.

Cross relaxation at the lysozyme–water interface: an NMR line-shape-relaxation correlation study

1984 ◽  
Vol 62 (10) ◽  
pp. 1002-1009 ◽  
Author(s):  
H. Peemoeller ◽  
D. W. Kydon ◽  
A. R. Sharp ◽  
L. J. Schreiner
Keyword(s):  
Relaxation Rate ◽  
Line Shape ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Cross Relaxation ◽  
Spin Lattice Relaxation ◽  
Water Proton ◽  
Water Interface ◽  
Spin Lattice Relaxation Rate ◽  
Spin Lattice

In wet hen egg white lysozyme (HEWL), the molecular dynamics at the lysozyme–water interface was studied using a proton NMR line-shape-relaxation correlation approach that employed selective inversion of the proton magnetization. The intrinsic lysozyme proton spin-lattice relaxation rate, the intrinsic water proton spin-lattice relaxation rate, and the lysozyme proton – water proton cross-relaxation rate were determined. The lysozyme proton – water proton intermolecular interaction couples these protons and contributes to spin-lattice relaxation as well. The results suggest that a minimum of three different correlation times are needed to characterize the water molecule dynamics in wet HEWL.

Surface Induced Spin-Lattice Relaxation of Water in Tricalcium Silicate Gels

1991 ◽  
Vol 46 (8) ◽  
pp. 697-699
Author(s):  
F. Milia ◽  
Y. Bakopoulos ◽  
Lj. Miljkovic
Keyword(s):  
Grain Size ◽  
Lattice Relaxation ◽  
Lattice Relaxation Time ◽  
Tricalcium Silicate ◽  
Proton Spin ◽  
Spin Lattice Relaxation ◽  
Water Proton ◽  
Hydration Time ◽  
Spin Lattice ◽  
Stage Process

AbstractThe water proton spin-lattice relaxation time and recovery function of exchangeable water was measured in tricalcium silicate (C3S) gels. The measurements were carried out as a function of the hydration time and grain size. Results show that the hydration of (C3S) is a two stage process. A model is developped

scholarly journals Proton spin–lattice relaxation rate in supercooled H2O and H2 17O under high pressure

1990 ◽  
Vol 93 (7) ◽  
pp. 4796-4803 ◽  
Author(s):  
E. W. Lang ◽  
D. Girlich ◽  
H.‐D. Lüdemann ◽  
L. Piculell ◽  
D. Müller
Keyword(s):  
High Pressure ◽  
Relaxation Rate ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Spin Lattice Relaxation ◽  
Spin Lattice Relaxation Rate ◽  
Spin Lattice ◽  
Lattice Relaxation Rate

A study of 14N relaxation and nitrogen–proton spin coupling in Watson–Crick base pair models through Fourier transform measurements on NH proton spin–lattice relaxation in the rotating frame

1979 ◽  
Vol 57 (9) ◽  
pp. 1075-1079 ◽  
Author(s):  
Michael E. Moseley ◽  
Peter Stilbs
Keyword(s):  
Relaxation Times ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Coupling Constants ◽  
Spin Coupling ◽  
Spin Lattice Relaxation ◽  
Indirect Measurements ◽  
Spin Lattice ◽  
Proton Coupling ◽  
Nuclear Spin Lattice Relaxation

Indirect measurements of nitrogen-14 nuclear spin-lattice relaxation times and direct proton coupling constants are presented together with carbon-13 T1 data for a series of alkyl-substituted nucleic acid bases and mixtures thereof in DMSO-d6. With the exception of the guanine NH nitrogen, which possibly experiences a decrease in the electric field gradient upon complexation with cytosine, no indications of significant changes in the electronic environment around the nitrogen nuclei were found for any combination of bases. Forsen–Hoffman spin saturation transfer experiments on the NH and NH2 protons are also presented.

An experimental evaluation of nonselective proton spin–lattice relaxation rates: analyses of data for the eight isomeric 2,3,4-tri-O-acetyl-1,6-anhydro-β-D-hexopyranoses

1980 ◽  
Vol 58 (18) ◽  
pp. 1916-1922 ◽  
Author(s):  
Klaus Bock ◽  
Laurance D. Hall ◽  
Christian Pedersen
Keyword(s):  
Lattice Relaxation ◽  
Proton Spin ◽  
Spin Lattice Relaxation ◽  
Proton Relaxation ◽  
Relaxation Rates ◽  
Pyranose Ring ◽  
Solvent Concentration ◽  
Relaxation Data ◽  
Spin Lattice ◽  
Single Set

The nonselective spin–lattice relaxation rates (R1-values) have been determined for all of the ring protons of the eight isomers of 2,3,4-tri-O-acetyl-1,6-anhydro-(β-D-hexopyranose as 0.1 molar solutions in benzene-d6. The effects on the proton R1-values of changes in solvent, concentration, temperature, and proton impurities are documented and 13C R1-values are given to show that the first two sets of variations are due to changes in motional correlation times of the molecules. The proton relaxation data can be fitted by regressional analyses to a single set of interproton relaxation contributions, the numerical values of which accord with a 1C4 conformation for the pyranose ring somewhat distorted by the 1,6-anhydro bridge.

The water proton spin-lattice relaxation times in virus-infected cells

1979 ◽  
Vol 10 (2) ◽  
pp. 143-146 ◽  
Author(s):  
Gianni Valensin ◽  
Elena Gaggelli ◽  
Enzo Tiezzi ◽  
Pier Egisto Valensin ◽  
Maria L.Bianchi Bandinelli
Keyword(s):  
Relaxation Times ◽  
Lattice Relaxation ◽  
Proton Spin ◽  
Spin Lattice Relaxation ◽  
Water Proton ◽  
Spin Lattice ◽  
Infected Cells
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