Galactomannans of Trichosporon fermentans and other yeasts; proton magnetic resonance and chemical studies

1968 ◽  
Vol 46 (13) ◽  
pp. 2299-2304 ◽  
Author(s):  
P. A. J. Gorin ◽  
J. F. T. Spencer
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Main Chain ◽  
Resonance Spectrum ◽  
Proton Magnetic Resonance Spectrum ◽  
Yeast Cells ◽  
Side Chains ◽  
Trichosporon Fermentans ◽  
Chemical Studies ◽  
Magnetic Resonance Spectra

A survey of the alkali soluble polysaccharides of yeast cells shows that galactomannans are formed by certain species of the genera Trichosporon, Schizosaccharomyces, and Nadsonia. Different species of Trichosporon form galactomannans, pentosylmannans, and a mannan. The galactomannans are distinguishable from those of Schizosaccharomyces by the H-1 region of their proton magnetic resonance spectra (recorded at 70° in D2O using an HA-100 Varian spectrometer), but the spectra of Trichosporon hellenicum and Nadsonia fulvescens galactomannans are virtually indistinguishable.The proton magnetic resonance spectrum of the Trichosporon fermentans galactomannan contains fewer signals than those of other Trichosporon galactomannans. The polymer consists predominantly of a main-chain containing a high proportion of (1 → 6)-mannopyranosyl units substituted in their 2-positions by O-α-D-galactopyranosyl-(1 → 2)-O-α-D-mannopyranosyl side-chains (1).

Structures of yeast mnnans containing both α- and β-linked D-mannopyranose units

1969 ◽  
Vol 47 (9) ◽  
pp. 1499-1505 ◽  
Author(s):  
P. A. J. Gorin ◽  
J. F. T. Spencer ◽  
S. S. Bhattacharjee
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Main Chain ◽  
Specific Rotation ◽  
Substantial Proportion ◽  
Side Chains ◽  
Structural Investigations ◽  
Magnetic Resonance Spectra

Several mannans from yeasts give proton magnetic resonance spectra with distinctive H-1 signals at higher field than τ 4.55. A number of these mannans were investigated polarimetrically and each has a specific rotation corresponding to a mixture of α- and β-D-linkages. Four were partially hydrolyzed and shown to have most of the α-linkages in the main chains and the β-linkages in the side chains. Detailed structural investigations showed that the Pichiapastoris mannan probably has an α-(1→6)-D-manno-pyranosyl main-chain substituted in the 2-positions with a substantial proportion of O-α-D-mannopy-ranosyl-(1→2)-O-β-D-mannopyranosyl-(1→2)-O-β-D-mannopyranosyl-(1→2)-O-α-mannopyranosyl side-chains (1). The related mannan from Citeromycesmatritensis has a similar main-chain, which is substituted in the 2-positions by α-D-mannopyranosyl units and β-(1→2)-pyranose-linked D-mannotriosyl and perhaps mannobiosyl side-chains (2).

The Proton Magnetic Resonance Spectrum and Structure of Himgravine

1961 ◽  
Vol 14 (1) ◽  
pp. 64 ◽  
Author(s):  
RJ Abraham ◽  
HJ Bernstein
Keyword(s):  
Magnetic Resonance ◽  
High Resolution ◽  
Proton Magnetic Resonance ◽  
Resonance Spectrum ◽  
Proton Magnetic Resonance Spectrum ◽  
Relative Orientation ◽  
Other Information ◽  
Magnetic Resonance Spectra

The high resolution proton magnetic resonance spectra of himgravine and himbacine have been obtained in CHCl3 solution at 60 Mc/s. The spectra are consistent with only one of the two possible structures proposed. Other information with regard to relative orientation of groups within the molecule has also been obtained.

PROTON MAGNETIC RESONANCE SPECTROSCOPY AND THE SULPHATION OF LINEAR AND BRANCHED DEXTRAN

1963 ◽  
Vol 41 (3) ◽  
pp. 777-782 ◽  
Author(s):  
W. M. Pasika ◽  
L. H. Cragg
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Proton Magnetic Resonance ◽  
Proton Magnetic Resonance Spectroscopy ◽  
Resonance Spectrum ◽  
Chain Scission ◽  
Proton Magnetic Resonance Spectrum ◽  
Resonance Spectroscopy ◽  
Peak Areas ◽  
Magnetic Resonance Spectra

The introduction of sulphate groups into dextran produces characteristic changes in its proton magnetic resonance spectrum. With linear dextran a new signal appears which can be attributed solely to an effect of sulphate groups; with branched dextran this signal coincides with that due to the branching. From a comparison of peak areas in these spectra with peak areas in the spectra of the unsulphated linear and branched dextrans it is concluded that during sulphation no appreciable degradation occurred, whether by chain scission or branch hydrolysis.The proton magnetic resonance spectra also provide evidence for preferential substitution of sulphate at carbon 2 in the anhydroglucose unit.

THE ANALYSIS OF NUCLEAR MAGNETIC RESONANCE SPECTRA: IV. FOUR NUCLEI: AB3

1958 ◽  
Vol 36 (9) ◽  
pp. 1302-1307 ◽  
Author(s):  
R. J. Abraham ◽  
J. A. Pople ◽  
H. J. Bernstein
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Resonance Spectrum ◽  
Proton Magnetic Resonance Spectrum ◽  
Methyl Mercaptan ◽  
Nuclear Magnetic Resonance Spectra ◽  
Magnetic Resonance Spectra ◽  
Nuclear Magnetic

The proton magnetic resonance spectrum of methyl mercaptan has been analyzed on the basis of the nuclear grouping AB3. The spectrum calculated for [Formula: see text] agrees very well with that observed.

Proton magnetic resonance spectrum of 4-pyrone, chromone and xanthone

1966 ◽  
Vol 22 (7) ◽  
pp. 1391
Author(s):  
Caroline T. Mathis ◽  
J.H. Goldstein
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Resonance Spectrum ◽  
Proton Magnetic Resonance Spectrum
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