Electron spin resonance studies of the reactions of sorbate with nitrite in the presence of a spin trap

1982 ◽  
Vol 60 (12) ◽  
pp. 1598-1601 ◽  
Author(s):  
Avinash Joshi ◽  
Dennis M Hinton ◽  
George C Yang
Keyword(s):  
Electron Spin Resonance ◽  
Double Bond ◽  
Sodium Nitrite ◽  
Spin Trap ◽  
Spin Trapping ◽  
Potassium Sorbate ◽  
Radical Product ◽  
Rapid Decomposition ◽  
Esr Spectrum ◽  
Spin Resonance

An esr spectrum was generated when a spin trap, t-nitroso butane (tNB), was added to potassium sorbate solution at pH of 6.4. This spectrum can be explained by the abstraction of allylic hydrogen by NO2, generated by the decomposition of tNB, followed by spin trapping of the radical product. With increasing concentration of NO2, which was accomplished by either the addition of sodium nitrite or by lowering the pH to cause rapid decomposition of tNB, a different esr spectrum was observed. This spectrum can be accounted for by a radical formed by addition of NO2 to the 4,5 double bond of the sorbate and subsequent trapping by tNB.

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

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