Quantification and diversity of the archaeal community in a landfill site

2003 ◽  
Vol 49 (1) ◽  
pp. 28-36 ◽  
Author(s):  
Koji Mori ◽  
Richard Sparling ◽  
Masahiro Hatsu ◽  
Kazuhiro Takamizawa

At a sea-based, solid waste disposal site, methanogenic organisms were quantified by molecular approaches. The samples collected for analysis were from anaerobic leachate of the landfill site. When the DNA extracted from the leachate was examined by a quantitative PCR method using domain-specific 16S rDNA primers, archaeal DNA represented 2–3% of the total extracted DNA. On the basis of cloning and sequence comparison of the archaeal PCR products, more than half of the sequences belonged to Euryarchaeota, particularly relatives of the genus Methanosaeta. The cloning analysis suggested that the majority of methane emitted from the landfill site originated from the acetate-utilizing Methanosaeta.Key words: landfill, methanogen, archaea, 16S rDNA.

Epidemiology ◽  
2009 ◽  
Vol 20 ◽  
pp. S215-S216
Author(s):  
Janeth Mosquera-Becerra ◽  
Olga Gómez-Gutiérrez ◽  
Fabián Méndez

2007 ◽  
Vol 30 (11) ◽  
pp. 617-620 ◽  
Author(s):  
Taro URASE ◽  
Jiro TAKEMURA ◽  
Hiroyuki OKUMURA ◽  
Samerjai PANYOSARANYA ◽  
Chart CHIEMCHAISRI ◽  
...  

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