Fish embryo culture: observations on axial cord differentiation in presomitic isolates of the zebrafish Brachydanio rerio (Hamilton-Buchanan)

1982 ◽  
Vol 60 (7) ◽  
pp. 1710-1721 ◽  
Author(s):  
Hans W. Laale
Keyword(s):  
Embryo Culture ◽  
Nutrient Medium ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Fish Embryos ◽  
Meristic Variation

Eighteen pre- and post-rhombencephalic axial isolates from zebrafish, Brachydanio rerio, presomitic embryos divested of epidermis and yolk were maintained in a nutrient medium for 14 days at 24 ± 2 °C. The paper focuses on the differentiation of the notochord and metamerism of axial somites in culture. The technique is proposed as a possible means for studying parameters pertaining to meristic variation in developing fish embryos.

Fish embryo culture: cardiac monolayers and contractile activity in embryo explants from the zebrafish, Brachydanio rerio

1984 ◽  
Vol 62 (5) ◽  
pp. 878-885 ◽  
Author(s):  
Hans Willer Laale
Keyword(s):  
Embryo Culture ◽  
Nutrient Medium ◽  
Contractile Activity ◽  
Functional Differentiation ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Embryo Cultures ◽  
Culture Substrate ◽  
First Time ◽  
Cardiac Mesoderm

Stage 16+ axial isolates from embryos of the zebrafish, Brachydanio rerio (Hamilton-Buchanan), were separated from their yolk spheres and epidermis. Rhombencephalic level explants, with intact bilateral cardiac rudiments, were maintained in nutrient medium for 20 days at 24 ± 2 °C. All embryo cultures became attached to the culture substrate and underwent subsequent differentiation. Ten out of 25 explants showed bilateral migrations of determined but initially undifferentiated cardiac mesoderm. The progressive structural and functional differentiation of zebrafish cardiac monolayers and intact rudiments are described for the first time.

Fish embryo culture: rhombencephalic neuritic outgrowth in explanted axial cords from the zebrafish Brachydanio rerio (Hamilton-Buchanan)

1982 ◽  
Vol 60 (12) ◽  
pp. 3215-3219 ◽  
Author(s):  
Hans W. Laale
Keyword(s):  
Embryo Culture ◽  
Fiber Bundles ◽  
Target Cells ◽  
Neural Cells ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Mammalian Tissues ◽  
Neuritic Outgrowth ◽  
Close Contacts

Fifteen stage 17 axial cords from the zebrafish Brachydanio rerio were stripped of epidermis and separated from their yolk spheres. Posterior halves, from the mid-rhombencephalic level to the caudal knob, were maintained in nutrient medium for 30 days at 24 ± 2 °C. The study describes preliminary observations on rhombencephalic neuritic outgrowth and associations with non-neural cells from 7 to 30 days of culture. Nerve fiber bundles increased in number and complexity with time and displayed heterogeneous branching behaviour. Frequent multiple close contacts with individual non-neural cells were observed, but selectivity of attraction to specific target cells was not verified. The findings are compared with similar contacts reported for mammalian tissues in vitro.

In vitro primary spreading and dissociation of zebrafish, Brachydanio rerio, blastoderm-derived embryonic epidermis

1981 ◽  
Vol 59 (5) ◽  
pp. 800-811 ◽  
Author(s):  
Hans W. Laale
Keyword(s):  
Nutrient Medium ◽  
Relevant Literature ◽  
Cell Types ◽  
Brachydanio Rerio ◽  
Initial Attachment ◽  
Adhesive Properties ◽  
Membrane Formation ◽  
Locomotory Behaviour

Blastoderm isolates from Brachydanio rerio (Teleostei: Cyprinidae) blastulas were maintained in nutrient medium for up to 40 days. Of 120 blastoderm isolates cultured 75% showed initial attachment and underwent major changes in adhesive properties affecting aggregative and locomotory behaviour. Of the attached blastoderm isolates, 70% differentiated into embryos of varying degrees of organization and all exhibited subsequent primary membrane formation and spreading. The paper, covering the first 18 days of culture, focuses on the phenomenon of primary membrane spread formation, dissociation, and its associated cell types. A discussion of the relevant literature is provided.

scholarly journals 692 Plant Embryo Culture Techniques and Applications

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 518C-518
Author(s):  
Mark P. Bridgen
Keyword(s):  
Embryo Culture ◽  
Nutrient Medium ◽  
Zygotic Embryo ◽  
Embryo Rescue ◽  
Cultivated Plants ◽  
Breeding Cycle ◽  
Zygotic Embryos ◽  
Culture Techniques ◽  
Plant Embryo ◽  
Embryo Culture Techniques

Traditional and biotechnological breeding techniques are being united to develop exciting new plants and to improve existing cultivated plants by introducing natural variability from germplasm resources. Intervarietal, interspecific and intergeneric crosses can be accomplished by using plant embryo culture techniques, sometimes also referred to as embryo rescue. Embryo culture involves the isolation and growth of immature or mature zygotic embryos under sterile conditions on an aseptic nutrient medium with the goal of obtaining a viable plant. The technique depends on isolating the embryo without injury, formulating a suitable nutrient medium, and inducing continued embryogenic growth and seedling formation. The culture of immature embryos is used to rescue embryos from hybrid crosses that were once thought to be incompatible because they would normally abort or not undergo the progressive sequence of ontogeny. The culture of mature embryos from ripened seeds is used to eliminate seed germination inhibitors, to overcome dormancy restrictions, or to shorten the breeding cycle. New and exciting cultivars of Alstroemeria, also known as Lily-of-the-Incas, Inca Lily, or Peruvian Lily, have been bred by using zygotic embryo culture; these techniques and applications will be discussed.

ВПЛИВ ДЖЕРЕЛ АЗОТНОГО ЖИВЛЕННЯ НА СИНТЕЗ КАРОТИНОЇДІВ ДЕЯКИМИ ШТАМАМИ БАЗИДІОМІЦЕТІВ

2014 ◽  
Vol 4 (01) ◽  
pp. 22
Author(s):  
A. K. Veligodska ◽  
O. V. Fedotov ◽  
A. S. Petreeva
Keyword(s):  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Fluid ◽  
Carotenoid Content ◽  
Nitrogen Compounds ◽  
Total Carotenoid ◽  
Carotenoid Accumulation ◽  
Total Carotenoid Content ◽  
Peptone Medium ◽  
Acetone Extracts

<p>The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula.</p> <p>As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO<sub>3</sub>. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified.</p> <p>Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain <em>L. sulphureus</em> Ls-08, and in a concentration of 4 g/l by strains of <em>F. hepatica </em>Fh-18 and <em>F. fomentarius</em> Ff-1201.</p> <p>In order to increase the accumulation of carotenoids in the mycelium  we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of <em>L. sulphureus</em> Ls- 08 strain; alanine for <em>F. fomentarius</em> Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of <em>F. hepatica</em>. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids.</p> <p><em>Keywords: nitrogen-containing substances, Basidiomycetes, mycelium</em><em>,</em><em> culture filtrate, carotenoids</em></p>

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