Protective Effects of Water Extract of Clam on Normal and CCl4-Induced Damage in Primary Cultured Rat Hepatocytes

2010 ◽  
Vol 38 (06) ◽  
pp. 1193-1205 ◽  
Author(s):  
H.M. Chi ◽  
S.T. Chou ◽  
S.C. Lin ◽  
Z.Y. Su ◽  
Lee-Yan Sheen
Keyword(s):  
Lactate Dehydrogenase ◽  
Carbon Tetrachloride ◽  
Water Extract ◽  
Rat Hepatocytes ◽  
Control Group ◽  
Protective Effects ◽  
Primary Cultured Rat Hepatocytes ◽  
Defense Systems ◽  
Intracellular Glutathione ◽  
Cultured Rat Hepatocytes

The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4 -induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ~ 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl4) for 1 hour, at concentrations of WEC between 0.42 ~ 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4 -treated control group ( p < 0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes.

scholarly journals Protective Effects of Intracellular Glutathione against Methylmercury Cytotoxicity in Primary Cultured Rat Hepatocytes.

Sangyo Igaku ◽  
1993 ◽  
Vol 35 (2) ◽  
pp. 124-125
Author(s):  
Keiichi TANNO ◽  
Toshiyuki FUKAZAWA ◽  
Shizuko TAJIMA ◽  
Kang-min YAN ◽  
Maki NAKAYAMA ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Protective Effects ◽  
Primary Cultured Rat Hepatocytes ◽  
Intracellular Glutathione ◽  
Cultured Rat Hepatocytes

scholarly journals Effect of P450-Inducers on the Hepatotoxicity of 2-Hexenal on the Leakage of Lactate Dehydrogenase from Primary Cultured Rat Hepatocytes.

Eisei kagaku ◽  
1997 ◽  
Vol 43 (6) ◽  
pp. 331-335
Author(s):  
Teruhisa HIRAYAMA ◽  
Akihiro YOSHIKAWA ◽  
Terue KASAI ◽  
Tetsushi WATANABE ◽  
Shunjiro OGAWA
Keyword(s):  
Lactate Dehydrogenase ◽  
Rat Hepatocytes ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes

Prostaglandins protect primary cultured rat hepatocytes against carbon tetrachloride-induced damage

1989 ◽  
Vol 24 (4) ◽  
pp. 447-447
Author(s):  
H. Yamamoto ◽  
Y. Kondo ◽  
T. Kawabe ◽  
K. Okano ◽  
R. Sassa ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Hepatocytes ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes

scholarly journals Hepatoprotective effect of galangin on carbon tetrachloride-induced hepatotoxicity via the LKB1/AMPK pathway

2021 ◽  
Vol 19 ◽  
pp. 205873922110008
Author(s):  
Meng Chen ◽  
Xinyan Song ◽  
Jifang Jiang ◽  
Lei Xing ◽  
Pengfei Wang
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Group Model ◽  
Protective Effects ◽  
Model Group ◽  
Expression Levels

To investigate the protective effects of galangin on liver toxicity induced by carbon tetrachloride (CCl4) in mice. Mouse hepatotoxicity model was established by intraperitoneal injection (i.p.) of 10 ml/kg body weight CCl4 that diluted with corn oil to a proportion of 1:500 on Kunming mice. The mice were randomly divided into five groups named control group, model group, and 1, 5, and 10 mg/kg galangin group. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed by ELISA. Liver histopathological examination was observed via optical microscopy. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and glutathion (GSSG) were analyzed to assess oxidative stress. Finally, western blot assay was carried out to analyse the expression levels of total AMP-activated protein kinase (AMPK), phospho-AMPK (p-AMPK), total liver kinase B1 (LKB1), and phospho-LKB1 (p-LKB1). Compared with the control group, in the model group, the levels of AST, ALT, MDA, and GSSG increased significantly ( p < 0.01); the activity of SOD and GSH decreased significantly ( p < 0.01); and the histopathological examination revealed liver necrosis. However, treatment with galangin (5 and 10 mg/kg) significantly reversed these CCl4-induced liver damage indicators. Furthermore, treatment with galangin (10 mg/kg) significantly increased the p-AMPK and p-LKB1 expression levels ( p < 0.01). This study supports the hepatoprotective effect of galangin against hepatotoxicity, perhaps occurring mainly through the LKB1/AMPK-mediated pathway.

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