Biofunctionalization of Carbon Nanotube Electrodes for the Fabrication of Biosensors and Biofuel Cells

2011 ◽  
Keyword(s):  
Carbon Nanotube ◽  
Biofuel Cells

Development of Biofuel Cell Using a Complex of Highly Oriented Immobilized His-Tagged Enzyme and Carbon Nanotube Surface Through a Pyrene Derivative

2019 ◽  
Vol 19 (6) ◽  
pp. 3551-3557 ◽  
Author(s):  
Hiroaki Sakamoto ◽  
Ayako Koto ◽  
Ei-Ichiro Takamura ◽  
Hitoshi Asakawa ◽  
Takeshi Fukuma ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Carbon Nanotube ◽  
Glucose Dehydrogenase ◽  
Molecular Layer ◽  
Biofuel Cells ◽  
Biofuel Cell ◽  
Similar Process ◽  
Enzyme Complex ◽  
Catalytic Current ◽  
Oriented Immobilization

For increasing the output of biofuel cells, increasing the cooperation between enzyme reaction and electron transfer on the electrode surface is essential. Highly oriented immobilization of enzymes onto a carbon nanotube (CNT) with a large specific surface area and excellent conductivity would increase the potential for their application as biosensors and biofuel cells, by utilizing the electron transfer between the electrode-molecular layer. In this study, we prepared a CNT-enzyme complex with highly oriented immobilization of enzyme onto the CNT surface. The complex showed excellent electrical characteristics, and could be used to develop biodevices that enable efficient electron transfer. Multi-walled carbon nanotubes (MWCNT) were dispersed by pyrene butyric acid N-hydroxysuccinimide ester, and then N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) and NiCl2 were added to modify the NTA-Ni2+ complex on the CNT surface. Pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) was immobilized on the CNT surface through a genetically introduced His-tag. Formation of the MWCNT-enzyme complex was confirmed by monitoring the catalytic current electrochemically to indicate the enzymatic activity. PQQ-GDH was also immobilized onto a highly ordered pyrolytic graphite surface using a similar process, and the enzyme monolayer was visualized by atomic force microscopy to confirm its structural properties. A biofuel cell was constructed using the prepared CNT-enzyme complex and output evaluation was carried out. As a result, an output of 32 μW/cm2 could be obtained without mediators.

Carbon Nanotube-based, Membrane-less and Mediator-free Enzymatic Biofuel Cells

2019 ◽  
Vol 19 (13) ◽  
pp. 55-60
Author(s):  
Shiunchin Wang ◽  
Anitha Patlolla ◽  
Zafar Iqbal
Keyword(s):  
Carbon Nanotube ◽  
Biofuel Cells ◽  
Enzymatic Biofuel Cells

scholarly journals Mediatorless high-power glucose biofuel cells based on compressed carbon nanotube-enzyme electrodes

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Abdelkader Zebda ◽  
Chantal Gondran ◽  
Alan Le Goff ◽  
Michael Holzinger ◽  
Philippe Cinquin ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
High Power ◽  
Biofuel Cells ◽  
Enzyme Electrodes

Carbon nanotube/enzyme biofuel cells

2012 ◽  
Vol 82 ◽  
pp. 179-190 ◽  
Author(s):  
Michael Holzinger ◽  
Alan Le Goff ◽  
Serge Cosnier
Keyword(s):  
Carbon Nanotube ◽  
Biofuel Cells

scholarly journals Chitosan improves stability of carbon nanotube biocathodes for glucose biofuel cells

2014 ◽  
Vol 50 (93) ◽  
pp. 14535-14538 ◽  
Author(s):  
Sarra El Ichi ◽  
Abdelkader Zebda ◽  
Awatef Laaroussi ◽  
Nadège Reverdy-Bruas ◽  
Didier Chaussy ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Electrocatalytic Activity ◽  
Oxygen Diffusion ◽  
Biofuel Cells

We demonstrate a novel combined chitosan–carbon-nanotube–enzyme biocathode with a fibrous microstructure that improves the performance by creating a protective microenvironment, preventing the loss of the electrocatalytic activity of the enzyme, and providing good oxygen diffusion.

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