scholarly journals Fermentable fibers induce rapid macro- and micronutrient depletion in Toll-like receptor 5-deficient mice

2020 ◽  
Vol 318 (5) ◽  
pp. G955-G965
Author(s):  
Rachel M. Golonka ◽  
Beng San Yeoh ◽  
Yaqi Li ◽  
Piu Saha ◽  
Ahmed A. Abokor ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Genetic Manipulation ◽  
Biliary Disease ◽  
Micronutrient Deficiencies ◽  
Toll Like Receptor ◽  
Chemical Induction ◽  
Novel Approach ◽  
Fiber Diet ◽  
Toll Like Receptor 5 ◽  
Deficient Mice

Feeding a dietary, fermentable fiber diet to a subset of Toll-like receptor 5 deficient (T5KO) mice induces early onset hyperbilirubinemia and cholemia that later manifests to hepatocellular carcinoma (HCC). Our study highlights that fermentable fiber-induced cholestasis is characterized with modest macro- and micronutrient deficiencies that may further contribute to hepatic biliary disease. Compared with chemical induction, immunization, surgery, or genetic manipulation, these findings provide a novel approach to study the cholestatic subtype of HCC.

scholarly journals Attenuation of phagocytosis of xenogeneic cells by manipulating CD47

Blood ◽  
2006 ◽  
Vol 109 (2) ◽  
pp. 836-842 ◽  
Author(s):  
Hui Wang ◽  
Jon VerHalen ◽  
Maria Lucia Madariaga ◽  
Shuanglin Xiang ◽  
Shumei Wang ◽  
...  
Keyword(s):  
Genetic Manipulation ◽  
Regulatory Protein ◽  
Wild Type Mouse ◽  
Xenograft Rejection ◽  
Novel Approach ◽  
Mouse Macrophages ◽  
Mouse Cells ◽  
Deficient Mice

Abstract Signal regulatory protein α (SIRPα) is a critical immune inhibitory receptor on macrophages, and its interaction with CD47, a ligand for SIRPα, prevents autologous phagocytosis. We hypothesized that interspecies incompatibility of CD47 may contribute to the rejection of xenogeneic cells by macrophages. Here, we show that pig CD47 does not interact with mouse SIPRα. Similar to CD47−/− mouse cells, porcine red blood cells (RBCs) failed to induce SIRPα tyrosine phosphorylation in mouse macrophages. Blocking SIRPα with antimouse SIRPα mAb (P84) significantly enhanced the phagocytosis of CD47+/+ mouse cells, but did not affect the engulfment of porcine or CD47−/− mouse cells by mouse macrophages. CD47-deficient mice, whose macrophages do not phagocytose CD47−/− mouse cells, showed markedly delayed clearance of porcine RBCs compared with wild-type mouse recipients. Furthermore, mouse CD47 expression on porcine cells markedly reduced their phagocytosis by mouse macrophages both in vitro and in vivo. These results indicate that interspecies incompatibility of CD47 contributes significantly to phagocytosis of xenogeneic cells by macrophages and suggest that genetic manipulation of donor CD47 to improve its interaction with the recipient SIRPα may provide a novel approach to prevent phagocyte-mediated xenograft rejection.

Toll-like receptor 5 deficiency protects from wasting disease in a T cell transfer colitis model in T cell receptor-β-deficient mice

2012 ◽  
Vol 18 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Gijs Hardenberg ◽  
Yu Yao ◽  
Ciriaco A. Piccirillo ◽  
Megan K. Levings ◽  
Theodore S. Steiner
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Cell Transfer ◽  
Toll Like Receptor ◽  
Wasting Disease ◽  
Toll Like Receptor 5 ◽  
T Cell Transfer ◽  
Deficient Mice ◽  
Colitis Model

scholarly journals A genetic variant in toll‐like receptor 5 is linked to chemokine levels and hepatocellular carcinoma in steatohepatitis

2021 ◽  
Author(s):  
Hans Dieter Nischalke ◽  
Janett Fischer ◽  
Alexandra Klüners ◽  
Madlen Matz‐Soja ◽  
Benjamin Krämer ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Genetic Variant ◽  
Toll Like Receptor ◽  
Toll Like Receptor 5

Toll‐like receptor 5 and 8 in hepatocellular carcinoma

Apmis ◽  
2021 ◽  
Author(s):  
Valtteri Kairaluoma ◽  
Niko Kemi ◽  
Heikki Huhta ◽  
Vesa‐Matti Pohjanen ◽  
Olli Helminen
Keyword(s):  
Hepatocellular Carcinoma ◽  
Toll Like Receptor ◽  
Toll Like Receptor 5

scholarly journals Gnotobiotic Toll-Like Receptor 5 Deficient Mice Lack Spontaneous Colitis and Metabolic Syndrome

2011 ◽  
Vol 140 (5) ◽  
pp. S-324
Author(s):  
Matam Vijay-Kumar ◽  
Jesse D. Aitken ◽  
Yueju Su ◽  
Gayathri Srinivasan ◽  
Frederic A. Carvalho ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Toll Like Receptor ◽  
Toll Like Receptor 5 ◽  
Deficient Mice

279 Altered Gut Microbiota in Toll-Like Receptor-5 (TLR5) Deficient Mice Results in Metabolic Syndrome

2010 ◽  
Vol 138 (5) ◽  
pp. S-52
Author(s):  
Matam Vijay-Kumar ◽  
Jesse D. Aitken ◽  
Frederic A. Carvalho ◽  
Tyler C. Cullender ◽  
Simon M. Mwangi ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Gut Microbiota ◽  
Toll Like Receptor ◽  
Toll Like Receptor 5 ◽  
Deficient Mice
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