Polarity and transport properties of rabbit kidney proximal tubule cells on collagen IV-coated porous membranes

1995 ◽  
Vol 269 (1) ◽  
pp. F22-F30 ◽  
Author(s):  
I. Genestie ◽  
J. P. Morin ◽  
B. Vannier ◽  
G. Lorenzon
Keyword(s):  
Proximal Tubule ◽  
Transport Properties ◽  
Collagen Iv ◽  
Transport Processes ◽  
Rabbit Kidney ◽  
Porous Membranes ◽  
Proximal Tubule Cells ◽  
Kidney Proximal Tubule ◽  
Apical Compartment ◽  
Tubule Cells

A high degree of functional polarity has been obtained in primary cultures of rabbit kidney proximal tubule cells grown on collagen IV-coated porous membranes. Tight confluency was attained 6 days after seeding and maintained for at least 6 more days, as shown by analysis of paracellular inulin diffusion. From day 6 onward, L-lactate, ammonia, and D-glucose concentration gradient and a pH difference of approximately 1 unit developed between the two nutrient medium compartments. Confluent monolayers expressed organic ion transport properties higher than those formerly reported for other cell models. Transcellular transport of 20 microM tetraethylammonium was directed from basal to apical compartment and was specifically inhibited by mepiperphenidol (1 mM). Unidirectional transport of 2.4 microM p-aminohippurate also occurred from basal to apical compartment, was saturable, and specifically inhibited by probenecid (1 mM). These results suggest that rabbit kidney proximal tubule cells, cultured under the experimental conditions described here, may be a useful model for the in vitro study of highly polarized renal transport processes.

Changes in expression and subcellular localization of annexin IV in rabbit kidney proximal tubule cells during primary culture

1995 ◽  
Vol 165 (2) ◽  
pp. 313-322 ◽  
Author(s):  
D. Massey-Harroche ◽  
V. Traverso ◽  
N. Mayran ◽  
V. Francou ◽  
A. Vandewalle ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Proximal Tubule ◽  
Primary Culture ◽  
Rabbit Kidney ◽  
Proximal Tubule Cells ◽  
Kidney Proximal Tubule ◽  
Tubule Cells ◽  
Annexin Iv

Hyposmotic activation of Ca-activated K channels in cultured rabbit kidney proximal tubule cells

1991 ◽  
Vol 260 (1) ◽  
pp. F27-F33 ◽  
Author(s):  
K. Kawahara ◽  
A. Ogawa ◽  
M. Suzuki
Keyword(s):  
Proximal Tubule ◽  
Single Cells ◽  
Cell Volume Regulation ◽  
Cell Swelling ◽  
Rabbit Kidney ◽  
Proximal Tubule Cells ◽  
K Channels ◽  
Kidney Proximal Tubule ◽  
Tubule Cells ◽  
K Currents

The effects of hypotonicity on K currents were examined in the single cells isolated from cultured rabbit kidney proximal tubule cells. Using the whole cell clamp technique [pipette-filling solution was 145 mM KCl with 0 Ca plus 0.08 mM ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid], we found that the K currents increased from 0.8 to 43.5 +/- 12.5 pA at a holding potential (Vp) = 0 mV in response to reduction of extracellular osmolality from 290 to 218 mosmol/kgH2O (n = 8). Removal of Ca from bath reduced osmotic activation of K currents by 74% (n = 10). Application of 5 mM Ba to the bath completely inhibited the K currents at Vp = 0 mV but not at Vp = 60 mV. Dominant K currents were shown to be Ca-activated (maxi) K channels by their single-channel conductance (175 +/- 8.6 pS) and Ca dependence. Relative permeability ratio (PK/PNa) was 9. In conclusion, hypotonic-induced cell swelling stimulates Ca-activated K channels, which may play a role in cell volume regulation.

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