scholarly journals Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index

2006 ◽  
Vol 25 (3) ◽  
pp. 514-524 ◽  
Author(s):  
Wilfrid Carre ◽  
Xiaofei Wang ◽  
Tom E. Porter ◽  
Yves Nys ◽  
Jianshan Tang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Dna Microarrays ◽  
Chicken Genome ◽  
Expressed Sequence Tag ◽  
Model Organism ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Chicken Gene ◽  
Chicken Genome Sequence ◽  
Gene Index

Its accessibility, unique evolutionary position, and recently assembled genome sequence have advanced the chicken to the forefront of comparative genomics and developmental biology research as a model organism. Several chicken expressed sequence tag (EST) projects have placed the chicken in 10th place for accrued ESTs among all organisms in GenBank. We have completed the single-pass 5′-end sequencing of 37,557 chicken cDNA clones from several single and multiple tissue cDNA libraries and have entered 35,407 EST sequences into GenBank. Our chicken EST sequences and those found in public databases (on July 1, 2004) provided a total of 517,727 public chicken ESTs and mRNAs. These sequences were used in the CAP3 assembly of a chicken gene index composed of 40,850 contigs and 79,192 unassembled singlets. The CAP3 contigs show a 96.7% match to the chicken genome sequence. The University of Delaware (UD) EST collection (43,928 clones) was assembled into 19,237 nonredundant sequences (13,495 contigs and 5,742 unassembled singlets). The UD collection contains 6,223 unique sequences that are not found in other public EST collections but show a 76% match to the chicken genome sequence. Our chicken contig and singlet sequences were annotated according to the highest BlastX and/or BlastN hits. The UD CAP3 contig assemblies and singlets are searchable by nucleotide sequence or key word ( http://cogburn.dbi.udel.edu ), and the cDNA clones are readily available for distribution from the chick EST website and clone repository ( http://www.chickest.udel.edu ). The present paper describes the construction and normalization of single and multiple tissue chicken cDNA libraries, high-throughput EST sequencing from these libraries, the CAP3 assembly of a chicken gene index from all public ESTs, and the identification of several nonredundant chicken gene sets for production of custom DNA microarrays.

Genomics: The chicken genome sequence

Heredity ◽  
2005 ◽  
Vol 94 (6) ◽  
pp. 567-568 ◽  
Author(s):  
P Jensen
Keyword(s):  
Genome Sequence ◽  
Chicken Genome ◽  
Chicken Genome Sequence

scholarly journals Addition of the microchromosome GGA25 to the chicken genome sequence assembly through radiation hybrid and genetic mapping

BMC Genomics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 129 ◽  
Author(s):  
Marine Douaud ◽  
Katia Fève ◽  
Marie Gerus ◽  
Valérie Fillon ◽  
Suzanne Bardes ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Genome Sequence ◽  
Radiation Hybrid ◽  
Chicken Genome ◽  
Sequence Assembly ◽  
Genome Sequence Assembly ◽  
Chicken Genome Sequence

scholarly journals Identification of Potential Vaccine and Drug Target Candidates by Expressed Sequence Tag Analysis and Immunoscreening of Onchocerca volvulus Larval cDNA Libraries

2000 ◽  
Vol 68 (6) ◽  
pp. 3491-3501 ◽  
Author(s):  
Michelle Lizotte-Waniewski ◽  
Wilson Tawe ◽  
David B. Guiliano ◽  
Wenhong Lu ◽  
Jing Liu ◽  
...  
Keyword(s):  
Drug Targets ◽  
Expressed Sequence Tag ◽  
Proteinase Inhibitors ◽  
Gene Discovery ◽  
Onchocerca Volvulus ◽  
Detoxification Enzymes ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence

ABSTRACT The search for appropriate vaccine candidates and drug targets against onchocerciasis has so far been confronted with several limitations due to the unavailability of biological material, appropriate molecular resources, and knowledge of the parasite biology. To identify targets for vaccine or chemotherapy development we have undertaken two approaches. First, cDNA expression libraries were constructed from life cycle stages that are critical for establishment of Onchocerca volvulus infection, the third-stage larvae (L3) and the molting L3. A gene discovery effort was then initiated by random expressed sequence tag analysis of 5,506 cDNA clones. Cluster analyses showed that many of the transcripts were up-regulated and/or stage specific in either one or both of the cDNA libraries when compared to the microfilariae, L2, and both adult stages of the parasite. Homology searches against the GenBank database facilitated the identification of several genes of interest, such as proteinases, proteinase inhibitors, antioxidant or detoxification enzymes, and neurotransmitter receptors, as well as structural and housekeeping genes. Other O. volvulus genes showed homology only to predicted genes from the free-living nematode Caenorhabditis elegans or were entirely novel. Some of the novel proteins contain potential secretory leaders. Secondly, by immunoscreening the molting L3 cDNA library with a pool of human sera from putatively immune individuals, we identified six novel immunogenic proteins that otherwise would not have been identified as potential vaccinogens using the gene discovery effort. This study lays a solid foundation for a better understanding of the biology of O. volvulus as well as for the identification of novel targets for filaricidal agents and/or vaccines against onchocerciasis based on immunological and rational hypothesis-driven research.

Analysis of expressed sequence tags from roots of resistant soybean infected by the soybean cyst nematode

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 380-388 ◽  
Author(s):  
Nadim Alkharouf ◽  
Rana Khan ◽  
Benjamin Matthews
Keyword(s):  
Glycine Max ◽  
Soybean Cyst Nematode ◽  
Expressed Sequence Tag ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Post Inoculation ◽  
Resistance Response ◽  
Expressed Sequence

The soybean cyst nematode (SCN) Heterodera glycines is the most devastating pest of soybean in the U.S.A. The resistance response elicited by SCN in soybean is complex, and genes involved in the response to a large extent are unknown and not well characterized. We constructed cDNA libraries made from mRNA extracted from roots of the resistant soybean Glycine max L. Merr. 'Peking' at 12 h, 2 to 4 days, and 6 to 8 days post inoculation with the soybean cyst nematode, population NL1-RHp, similar to race 3. Expressed sequence tag analysis of the libraries provides rapid discovery of genes involved in the response of soybean to the nematode. A total of 3454 cDNA clones were examined from the three libraries, of which 25 cDNAs were derived from nematode RNA. The levels of certain stress-induced genes such as SAM22 and glutathione S-transferase (GST8) were elevated in the SCN-infected roots relative to uninoculated roots. Early defense response genes, particularly ascorbate peroxidase and lipoxygenase, were abundant in the 12-h library. By 6–8 days, the expression of most of those genes was not as abundant, whereas genes coding for unknown proteins and stress-induced proteins continued to be highly expressed. These ESTs and associated information will be useful to scientists examining gene and protein interactions between nematodes and plants.Key words: expressed sequence tag, gene expression, Glycine max, Heterodera glycines, plant-pathogen interaction, transcript profile.

scholarly journals Cloning and sequence analysis of cDNAs encoding the α1, α2 and α3 chains of mouse collagen VI

1993 ◽  
Vol 291 (3) ◽  
pp. 787-792 ◽  
Author(s):  
R Z Zhang ◽  
T C Pan ◽  
R Timpl ◽  
M L Chu
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequences ◽  
Untranslated Regions ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Collagen Vi ◽  
Central Segment ◽  
Glycosylation Sites ◽  
Key Features ◽  
Triple Helical Domain

cDNA clones encoding the alpha 1, alpha 2 and alpha 3 chains of mouse collagen VI have been isolated by screening cDNA libraries with the corresponding human probes. The composite cDNAs for the alpha 1, alpha 2, and alpha 3 chains are 2.5, 1.6 and 2.9 kb in size respectively. The alpha 1 and alpha 2 cDNAs encode the C-terminal portions of the chains as well as the entire 3′-untranslated regions, while the alpha 3 cDNAs encode a central segment of 959 amino acids flanking the triple-helical domain. The deduced amino acid sequences share 86-88% identity with the human counterparts and 67-73% identity with the chicken equivalents. Alignment of the deduced amino acid sequences of mouse, human and chicken collagens reveal that the key features of the protein, including the cysteine residues, imperfections in the Gly-Xaa-Xaa regions, Arg-Gly-Asp sequences and potential N-glycosylation sites, are mostly conserved.

scholarly journals Elucidating the genetic basis of an oligogenic birth defect using whole genome sequence data in a non-model organism, Bubalus bubalis

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Lynsey K. Whitacre ◽  
Jesse L. Hoff ◽  
Robert D. Schnabel ◽  
Sara Albarella ◽  
Francesca Ciotola ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Birth Defect ◽  
Genetic Basis ◽  
Sequence Data ◽  
Model Organism ◽  
Bubalus Bubalis ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Genome Sequence Data

scholarly journals Development of EST-Molecular Markers from RNA Sequencing for Genetic Management and Identification of Growth Traits in Potato Grouper (Epinephelus tukula)

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 36
Author(s):  
Te-Hua Hsu ◽  
Yu-Ting Chiu ◽  
Hung-Tai Lee ◽  
Hong-Yi Gong ◽  
Chang-Wen Huang
Keyword(s):  
Molecular Markers ◽  
Expressed Sequence Tag ◽  
De Novo ◽  
Growth Traits ◽  
Gene Diversity ◽  
Snp Markers ◽  
Cdna Libraries ◽  
Functional Markers ◽  
Genetic Management ◽  
Ssr And Snp Markers

The accuracy and efficiency of marker-assisted selection (MAS) has been proven for economically critical aquaculture species. The potato grouper (Epinephelus tukula), a novel cultured grouper species in Taiwan, shows large potential in aquaculture because of its fast growth rate among other groupers. Because of the lack of genetic information for the potato grouper, the first transcriptome and expressed sequence tag (EST)-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were developed. Initially, the transcriptome was obtained from seven cDNA libraries by using the Illumina platform. De novo transcriptome of the potato grouper yielded 51.34 Gb and 111,490 unigenes. The EST-derived SSR and SNP markers were applied in genetic management, in parentage analysis, and to discover the functional markers of economic traits. The F1 juveniles were identified as siblings from one pair of parents (80 broodstocks). Fast- and slow-growth individuals were analyzed using functional molecular markers and through their association with growth performance. The results revealed that two SNPs were correlated with growth traits. The transcriptome database obtained in this study and its derived SSR and SNP markers may be applied not only for MAS but also to maintain functional gene diversity in the novel cultured grouper.

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