scholarly journals Phadiatop Infant in the Diagnosis of Atopy in Children with Allergy-Like Symptoms

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Ragnhild Halvorsen ◽  
Åsa Jenner ◽  
Else Marie Hagelin ◽  
Magnus P. Borres
Keyword(s):  
Young Children ◽  
Clinical Information ◽  
Specific Ige ◽  
In Vitro Test ◽  
Blinded Manner ◽  
Specific Ige Antibodies ◽  
Vitro Test ◽  
Ige Mediated ◽  
Inhalant Allergens

Background and Objective. Allergy-like symptoms such as wheezing and eczema are common in young children and an early diagnosis is important to initiate correct management. The objective of this study was to evaluate the diagnostic performance of Phadiatop Infant, an in vitro test for determination of early sensitisation to food and inhalant allergens.Patients and Methods. The study was conducted, retrospectively, using frozen sera from 122 children (median age 2.7 years) admitted to the hospital with suspected allergic symptoms. The doctor's diagnosis atopic/nonatopic was based on routinely used procedures such as clinical evaluation, SPT, total and allergen-specific IgE antibodies. The performance of Phadiatop Infant was evaluated in a blinded manner against this diagnosis.Results. Eighty-four of the 86 children classified as atopic showed a positive Phadiatop Infant test. Thirty-six were classified as nonatopic, 32 of who had a negative test. With a prevalence of atopy of 70% in this population, this gives a sensitivity of 98%, a specificity of 89%, and a positive and negative predictive value of 95% and 94%, respectively.Conclusion. The results from the present study suggest that Phadiatop Infant could be recommended as a complement to the clinical information in the differential diagnosis on IgE-mediated disease in young children with allergy-like symptoms.

scholarly journals An in vitro Test for Detection of Specific IgE-mediated Penicillin Reactions in Children

1970 ◽  
Vol 4 (5) ◽  
pp. 436-436
Author(s):  
Zack H Haddad ◽  
Joel Korotzer ◽  
John James
Keyword(s):  
Specific Ige ◽  
In Vitro Test ◽  
Vitro Test ◽  
Ige Mediated

In vitro testing for allergic and immunologic diseases

2019 ◽  
Vol 40 (6) ◽  
pp. 480-484
Author(s):  
Elisa N. Ochfeld ◽  
Melanie Makhija
Keyword(s):  
Immune Cell ◽  
Allergic Diseases ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
In Vitro Tests ◽  
In Vitro Test ◽  
Ige Mediated ◽  
Lymphocyte Immunophenotyping ◽  
Immunologic Diseases

In vitro tests are used to assist in the diagnosis of both allergic and immunologic diseases. Unfortunately, there is no single test that is pathognomonic for most allergic diseases. The most commonly ordered in vitro test by allergists is allergen specific IgE (sIgE), which is used to help diagnose IgE mediated hypersensitivity to foods, aeroallergens and venoms. Multiple assays exist, although none of these assays have been adopted as the industry standard. Epicutaneous skin test is also a fundamental test in the diagnosis of IgE mediated hypersensitivity. In addition, total IgE, basophil activation test (BAT), and serum tryptase may also be useful in elucidating allergic diseases. Immunologists rely on laboratory testing to diagnose primary immunodeficiency diseases. These tests include serum quantitative immunoglobulins, lymphocyte immunophenotyping by flow cytometry and immune cell functional testing. Furthermore, genetic testing is invaluable in the diagnosis of many primary Immunodeficiencies.

Development Of An In Vitro Test For Triatoma Specific IgE

2010 ◽  
Vol 125 (2) ◽  
pp. AB202
Author(s):  
B.J. Goldberg ◽  
D. Lo ◽  
C. Weirauch ◽  
E. Macy ◽  
C. Lin
Keyword(s):  
Specific Ige ◽  
In Vitro Test ◽  
Vitro Test

scholarly journals New In-vitro Test for IgE-mediated Hypersensitivity in Man

BMJ ◽  
1972 ◽  
Vol 2 (5814) ◽  
pp. 623-624 ◽  
Author(s):  
M. W. Greaves ◽  
S. Yamamoto ◽  
V. M. Fairley
Keyword(s):  
In Vitro Test ◽  
Vitro Test ◽  
Ige Mediated

scholarly journals A clinical study of inhalant allergens in patients with allergic rhinitis

2017 ◽  
Vol 3 (2) ◽  
pp. 380
Author(s):  
Suhas Y. Choudhari ◽  
Aravind B. Sangavi
Keyword(s):  
Allergic Rhinitis ◽  
Specific Ige ◽  
Total Serum ◽  
In Vitro Test ◽  
Vitro Assay ◽  
Serum Ige ◽  
Dust Mite ◽  
Inhalant Allergens ◽  
High Degree

<p class="abstract"><strong>Background:</strong> The basis of diagnosis of allergy requires a good history and examination, however, the diagnosis cannot be confirmed on the basis of symptoms alone, because both allergic and non-allergic conditions can present with similar symptoms. Hence, allergy testing in the form of specific IgE (sIgE) measurement is an important aid in demonstrating both the presence and severity of such an allergy. The present study was undertaken to find out the common environmental allergens prevailing in Raichur causing allergic rhinitis, using carbohydrate cross reactive determinants (CCD), an in vitro test with high degree of sensitivity.</p><p class="abstract"><strong>Methods:</strong> The present prospective study was conducted among 30 patients with allergic rhinitis. A detailed general andENT examination were done, X-ray ofPNS, CT scan ofPNS, diagnostic nasal endoscopy and nasal smear examination for eosinophils, absolute eosinophil count and serum IgE levels using  Euroimmun system of in vitro assay of specific IgE antibodies.  </p><p class="abstract"><strong>Results:</strong> Allergy to dust mite, D. faranie, corn, carnation flower, sunflower, sheep wool and straw dust were the most frequent allergens causing allergic symptoms among patients in Raichur area.  Total serum IgE was elevated in all the patients, 60% were allergic to dust mite as found by anti CCD specific IgE.</p><p><strong>Conclusions:</strong> Antibodies to dust mite D. faranie, rye, T. mothy grass was the commonest finding. Identification of inhalant allergens is an important factor in prevention and treatment of allergic rhinitis. </p>

Immunoblotting technique: a new accurate in vitro test for detection of allergen-specific IgE in allergic rhinitis

2009 ◽  
Vol 266 (10) ◽  
pp. 1569-1573 ◽  
Author(s):  
Ossama A. Hamid ◽  
Shaheera Elfedawy ◽  
Sherif K. Mohamed ◽  
Hatem Mosaad
Keyword(s):  
Allergic Rhinitis ◽  
Specific Ige ◽  
In Vitro Test ◽  
Vitro Test ◽  
Immunoblotting Technique

Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation - An Analysis of Red Blood Cell Participation

1980 ◽  
Vol 44 (01) ◽  
pp. 006-008 ◽  
Author(s):  
D Bergqvist ◽  
K-E Arfors
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
In Vitro Test ◽  
Pharmacological Agents ◽  
Vitro Test ◽  
Releasing Effect ◽  
Plug Formation

SummaryIn a model using an isolated rabbit mesenteric preparation microvessels were transected and the time until haemostatic plugs formed was registered. Perfusion of platelet rich plasma gave no haemostasis whereas whole blood did. Addition of chlorpromazine or adenosine to the whole blood significantly prolonged the time for haemostasis, and addition of ADP to the platelet rich plasma significantly shortened it. It is concluded that red cells are necessary for a normal haemostasis in this model, probably by a combination of a haemodynamic and ADP releasing effect.The fundamental role of platelets in haemostatic plug formation is unquestionable but there are still problems concerning the stimulus for this process to start. Three platelet aggregating substances have been discussed – thrombin, adenosine diphosphate (ADP) and collagen. Evidence speaking in favour of thrombin is, however, very minimal, and the discussion has to be focused on collagen and ADP. In an in vitro system using polyethylene tubings we have shown that "haemostasis" can be obtained without the presence of collagen but against these results can be argued that it is only another in vitro test for platelet aggregation (1).To be able to induce haemostasis in this model, however, the presence of red blood cells is necessary. To further study this problem we have developed a model where haemostatic plug formation can be studied in the isolated rabbit mesentery and we have briefly reported on this (2).Thus, it is possible to perfuse the vessels with whole blood as well as with platelet rich plasma (PRP) and different pharmacological agents of importance.

scholarly journals In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis

2021 ◽  
Vol 9 (3) ◽  
pp. 478
Author(s):  
Ersilia Vita Fiscarelli ◽  
Martina Rossitto ◽  
Paola Rosati ◽  
Nour Essa ◽  
Valentina Crocetta ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
In Vitro Test ◽  
Chronic Infections ◽  
Hospital Environments ◽  
Vitro Test ◽  
General Reliability ◽  
Phage Activity

As disease worsens in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) colonizes the lungs, causing pulmonary failure and mortality. Progressively, PA forms typical biofilms, and antibiotic treatments determine multidrug-resistant (MDR) PA strains. To advance new therapies against MDR PA, research has reappraised bacteriophages (phages), viruses naturally infecting bacteria. Because few in vitro studies have tested phages on CF PA biofilms, general reliability remains unclear. This study aimed to test in vitro newly isolated environmental phage activity against PA isolates from patients with CF at Bambino Gesù Children’s Hospital (OBG), Rome, Italy. After testing in vitro phage activities, we combined phages with amikacin, meropenem, and tobramycin against CF PA pre-formed biofilms. We also investigated new emerging morphotypes and bacterial regrowth. We obtained 22 newly isolated phages from various environments, including OBG. In about 94% of 32 CF PA isolates tested, these phages showed in vitro PA lysis. Despite poor efficacy against chronic CF PA, five selected-lytic-phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, and Φ19_OBG) showed wide host activity. The Φ4_ZP1-meropenem and Φ14_OBG-tobramycin combinations significantly reduced CF PA biofilms (p < 0.001). To advance potential combined phage-antibiotic therapy, we envisage further in vitro test combinations with newly isolated phages, including those from hospital environments, against CF PA biofilms from early and chronic infections.

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