Photodynamic Therapy with the Silicon Phthalocyanine Pc 4 Induces Apoptosis in Mycosis Fungoides and Sezary Syndrome

Our current focus on the effects of Photodynamic Therapy (PDT) using silicon phthalocyanine Pc 4 photosensitizer on malignant T lymphocytes arose due to preclinical observations that Jurkat cells, common surrogate for human T cell lymphoma, were more sensitive to Pc 4-PDT-induced killing than epidermoid carcinoma A431 cells. Mycosis fungoides (MF) as well as Sezary syndrome (SS) are variants of cutaneous T-cell lymphoma (CTCL) in which malignant T-cells invade the epidermis. In this study, we investigated the cytotoxicity of Pc 4-PDT in peripheral blood cells obtained from patients with SS and in skin biopsies of patients with MF. Our data suggest that Pc 4-PDT preferentially induces apoptosis of CD4+CD7−malignant T-lymphocytes in the blood relative to CD11b+monocytes and nonmalignant T-cells.In vivoPc 4-PDT of MF skin also photodamages the antiapoptotic protein Bcl-2.

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Locomotion of T cells from patients with cutaneous T-cell lymphoma (Sezary syndrome and mycosis fungoides)

Cellular Immunology ◽

10.1016/0008-8749(80)90018-0 ◽

1980 ◽

Vol 50 (1) ◽

pp. 195-201 ◽

Cited By ~ 2

Author(s):

Sudhir Gupta ◽

Bijan Safai ◽

Richard Edelson ◽

Delphine Parrott ◽

Robert Good

Keyword(s):

T Cells ◽

T Cell ◽

Mycosis Fungoides ◽

Cell Lymphoma ◽

T Cell Lymphoma ◽

Sézary Syndrome ◽

Sezary Syndrome ◽

Cutaneous T Cell Lymphoma

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Cutaneous T-Cell Lymphoma: Mycosis Fungoides and Sézary Syndrome

Novel Therapeutics for Rare Lymphomas ◽

10.1007/978-3-030-25610-4_14 ◽

2019 ◽

pp. 221-246

Author(s):

Timothy J. Voorhees ◽

Edith V. Bowers ◽

Christopher R. Kelsey ◽

Yara Park ◽

Anne W. Beaven

Keyword(s):

T Cell ◽

Mycosis Fungoides ◽

Cell Lymphoma ◽

T Cell Lymphoma ◽

Sézary Syndrome ◽

Sezary Syndrome ◽

Cutaneous T Cell Lymphoma

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A new monoclonal antibody (CH-F42) recognizes a CD7- subset of normal T lymphocytes and circulating malignant cells in adult T-cell lymphoma- leukemia and Sezary syndrome

Blood ◽

10.1182/blood.v76.7.1361.bloodjournal7671361 ◽

1990 ◽

Vol 76 (7) ◽

pp. 1361-1368

Author(s):

WB Labastide ◽

MT Rana ◽

CR Barker

Keyword(s):

Monoclonal Antibody ◽

T Cell ◽

T Lymphocytes ◽

Cell Lymphoma ◽

T Cell Lymphoma ◽

Sézary Syndrome ◽

Sezary Syndrome ◽

Malignant Cells ◽

Activation Markers ◽

Normal Peripheral Blood

We describe a new rat immunoglobulin M monoclonal antibody (CH-F42) that recognizes a subset (1.5% to 8%) of normal peripheral blood T lymphocytes. The phenotype of these cells was determined, using dual- color immunofluorescence, to be CD2+, CD3+, CD4+, CD5+, CD7-, CD8-. They do not express T-cell activation markers, and are positive for UCHL1 (CD45RO), but negative for 2H4 (CD45RA). The antigen was expressed on circulating malignant cells in Sezary syndrome (four of four cases) and adult T-cell lymphoma-leukemia (ATLL) (four of six cases) and negative in a variety of other hematologic malignancies tested. These included chronic and acute lymphoid leukemias of B and T lineage, together with chronic and acute myeloid leukemias. However, normal CH-F42+ cells do not display any of the ultrastructural features associated with Sezary or ATLL cells. The marked similarities between these conditions together with the shared expression of an otherwise very restricted surface antigen (CH-F42) provide strong evidence for the existence of a common normal counterpart. Preliminary characterization studies of the antigen, which is also expressed by K562 and Jurkat cells, suggest the CH-F42 antigen is an O-linked, sialated glycan on a glycoprotein.

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