scholarly journals An Evaluation of MAPIA in Michigan as an Ante-Mortem Supplemental Test for Use in Suspect Tuberculosis Cattle

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Scott D. Fitzgerald ◽  
Heather A. Grodi ◽  
John B. Kaneene
Keyword(s):  
Bovine Tuberculosis ◽  
High Specificity ◽  
Tuberculosis Infection ◽  
Infection Status ◽  
Testing Methods ◽  
Veterinary Services ◽  
Serologic Test ◽  
Interpretation Criteria ◽  
Interpretation Criterion ◽  
Screening Assays

The objective of this study was to make use of bovine tuberculosis suspect cattle from the state of Michigan to validate a multiantigen print immunoassay for use on sera to serve as an improved supplementary ante-mortem test to increase specificity of current tuberculosis testing methods. Over a 27-month period, 234 sera were collected and tested by MAPIA method, which was evaluated using four different interpretation criteria. These results were subsequently compared to final mycobacterial culture and PCR results obtained by the National Veterinary Services Laboratories, Ames, IA, which served as the true indicator of the cattle’s tuberculosis infection status. This study indicates that an interpretation criterion which includes 3 or more positive reactions to the 11 different mycobacteria antigens utilized provided both an acceptable sensitivity (69.39%) and a high specificity (90.27%). This MAPIA technique shows potential for eventual application as a supplementary ante-mortem tuberculosis serologic test following one of the various current or soon-to-be-approved whole herd screening assays as part of a tuberculosis eradication program.

scholarly journals Denning behaviour of the European badger (Meles meles) correlates with bovine tuberculosis infection status

2012 ◽  
Vol 67 (3) ◽  
pp. 471-479 ◽  
Author(s):  
Nicola Weber ◽  
Stuart Bearhop ◽  
Sasha R. X. Dall ◽  
Richard J. Delahay ◽  
Robbie A. McDonald ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Meles Meles ◽  
Tuberculosis Infection ◽  
Infection Status ◽  
European Badger

scholarly journals A molecularly defined skin test reagent for the diagnosis of bovine tuberculosis compatible with vaccination against Johne’s Disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sonya Middleton ◽  
Sabine Steinbach ◽  
Michael Coad ◽  
Kevina McGill ◽  
Colm Brady ◽  
...  
Keyword(s):  
Skin Test ◽  
Recombinant Proteins ◽  
Bovine Tuberculosis ◽  
Synthetic Peptides ◽  
Blood Test ◽  
High Specificity ◽  
Active Components ◽  
Interferon Gamma Release Assays ◽  
Skin Responses

AbstractTuberculin Purified Protein Derivatives (PPDs) exhibit multiple limitations: they are crude extracts from mycobacterial cultures with largely unknown active components; their production depends on culture of mycobacteria requiring expensive BCL3 production facilities; and their potency depends on the technically demanding guinea pig assay. To overcome these limitations, we developed a molecularly defined tuberculin (MDT) by adding further antigens to our prototype reagent composed of ESAT-6, CFP-10 and Rv3615c (DIVA skin test, DST). In vitro screening using PBMC from infected and uninfected cattle shortlisted four antigens from a literature-based list of 18 to formulate the MDT. These four antigens plus the previously identified Rv3020c protein, produced as recombinant proteins or overlapping synthetic peptides, were formulated together with the three DST antigens into the MDT to test cattle experimentally and naturally infected with M. bovis, uninfected cattle and MAP vaccinated calves. We demonstrated significant increases in MDT-induced skin responses compared to DST in infected animals, whilst maintaining high specificity in unvaccinated or MAP vaccinated calves. Further, MDT can also be applied in in vitro blood-based interferon-gamma release assays. Thus, MDT promises to be a robust diagnostic skin and blood test reagent overcoming some of the limitations of PPDs and warrants full validation.

Prevalence and significant geospatial clusters of bovine tuberculosis infection at livestock–wildlife interface ecosystem in Eastern Tanzania

2013 ◽  
Vol 45 (5) ◽  
pp. 1223-1230 ◽  
Author(s):  
Richard Simon Mwakapuja ◽  
Zachariah Ephraim Makondo ◽  
Joseph Malakalinga ◽  
Ward Bryssinckx ◽  
Robinson Hammerthon Mdegela ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculosis Infection

P6019 Host genetics of resistance to bovine tuberculosis infection in dairy cows

2016 ◽  
Vol 94 (suppl_4) ◽  
pp. 157-158
Author(s):  
S. Wilkinson ◽  
S. C. Bishop ◽  
A. R. Allen ◽  
S. H. McBride ◽  
R. A. Skuce ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Bovine Tuberculosis ◽  
Tuberculosis Infection ◽  
Genetics Of Resistance ◽  
Host Genetics

scholarly journals Development and Validation of a Higher Throughput Screening Approach to Genotoxicity Testing Using the GADD45a-GFP GreenScreen HC Assay

2008 ◽  
Vol 14 (1) ◽  
pp. 16-30 ◽  
Author(s):  
Andrew W. Knight ◽  
Louise Birrell ◽  
Richard M. Walmsley
Keyword(s):  
Hazard Assessment ◽  
In Silico ◽  
Chemical Space ◽  
High Specificity ◽  
Screening Assays ◽  
Automated Screening ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds ◽  
Development And Validation ◽  
Low Solubility

There is a pressing need to develop rapid yet accurate screening assays for the identification of genotoxic liability and for early hazard assessment in drug discovery. The GADD45a-GFP human cell-based genotoxicity assay (GreenScreen HC) has been reformatted to test 12 compounds per 96-well microplate in a higher throughput, automated screening mode and the protocol applied to the analysis of 1266 diverse, pharmacologically active compounds. Testing from a fixed starting concentration of 100 µM and over 3 serial dilutions, the hit rates for genotoxicity (7.3%) and cytotoxicity (33%) endpoints of the assay have been determined in a much wider chemical space than previously reported. The degree of interference from color, autofluorescence, and low solubility has also been assessed. The assay results have been compared to an in silico approach to genotoxicity assessment using Derek for Windows software. Where carcinogenicity data were available, GreenScreen HC demonstrated a higher specificity than in silico methods while identifying genotoxic species that were not highlighted for genotoxic liability in structure-activity relationship software. Higher throughput screening from a fixed, low concentration reduces sensitivity to less potent genotoxins, but the maintenance of the previously reported high specificity is essential in early hazard assessment where misclassification can lead to the needless rejection of potentially useful compounds in drug development. ( Journal of Biomolecular Screening 2009:16-30)

Metastatic spinal deposits or bovine tuberculosis infection?

2015 ◽  
Vol 15 (3) ◽  
pp. S73
Author(s):  
Tom Marjoram ◽  
Saajid Kaleel
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculosis Infection

scholarly journals Surprisingly High Specificity of the PPD Skin Test for M. tuberculosis Infection from Recent Exposure in The Gambia

PLoS ONE ◽  
2006 ◽  
Vol 1 (1) ◽  
pp. e68 ◽  
Author(s):  
Philip C. Hill ◽  
Roger H. Brookes ◽  
Annette Fox ◽  
Dolly Jackson-Sillah ◽  
Moses D. Lugos ◽  
...  
Keyword(s):  
Skin Test ◽  
High Specificity ◽  
The Gambia ◽  
Tuberculosis Infection ◽  
Recent Exposure
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