Prevalence and significant geospatial clusters of bovine tuberculosis infection at livestock–wildlife interface ecosystem in Eastern Tanzania

The present study was carried out to investigate the diagnostic potential of gamma interferon (IFN-ã) assay and single intradermal comparative tuberculin test (SICTT), including species specification of bovine tuberculosis infection in different livestock farms of Assam and Meghalaya. A total of 199 animals (cattle and buffalo) were examined for bovine tuberculosis symptoms and swab samples were cultured. Biochemical tests and PCR were used for species specification of bovine tuberculosis. Out of 199 cases examined, 33 (16.58%) showed positive for SICTT, 39 (19.59%) for IFN-ã and 35(17.59%) for PCR. Based on PCR targeting pncA region, the confirmation was done for M. Bovis. IFN-ã thus ensures a sensitive and specific detection of early bovine tuberculosis infection together with SICTT and hence may be considered as a screening method of choice.

Download Full-text

Effects of Mycobacterium bovis on monocyte-derived macrophages from bovine tuberculosis infection and healthy cattle

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.2011.02304.x ◽

2011 ◽

Vol 321 (1) ◽

pp. 30-36 ◽

Cited By ~ 12

Author(s):

Yang Wang ◽

Xiangmei Zhou ◽

Jingjun Lin ◽

Fei Yin ◽

Lihua Xu ◽

...

Keyword(s):

Mycobacterium Bovis ◽

Bovine Tuberculosis ◽

Tuberculosis Infection ◽

Healthy Cattle

Download Full-text

An Evaluation of MAPIA in Michigan as an Ante-Mortem Supplemental Test for Use in Suspect Tuberculosis Cattle

Veterinary Medicine International ◽

10.1155/2012/674368 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6

Author(s):

Scott D. Fitzgerald ◽

Heather A. Grodi ◽

John B. Kaneene

Keyword(s):

Bovine Tuberculosis ◽

High Specificity ◽

Tuberculosis Infection ◽

Infection Status ◽

Testing Methods ◽

Veterinary Services ◽

Serologic Test ◽

Interpretation Criteria ◽

Interpretation Criterion ◽

Screening Assays

The objective of this study was to make use of bovine tuberculosis suspect cattle from the state of Michigan to validate a multiantigen print immunoassay for use on sera to serve as an improved supplementary ante-mortem test to increase specificity of current tuberculosis testing methods. Over a 27-month period, 234 sera were collected and tested by MAPIA method, which was evaluated using four different interpretation criteria. These results were subsequently compared to final mycobacterial culture and PCR results obtained by the National Veterinary Services Laboratories, Ames, IA, which served as the true indicator of the cattle’s tuberculosis infection status. This study indicates that an interpretation criterion which includes 3 or more positive reactions to the 11 different mycobacteria antigens utilized provided both an acceptable sensitivity (69.39%) and a high specificity (90.27%). This MAPIA technique shows potential for eventual application as a supplementary ante-mortem tuberculosis serologic test following one of the various current or soon-to-be-approved whole herd screening assays as part of a tuberculosis eradication program.

Download Full-text