Alterations of the Myovesical Plexus of the Human Overactive Detrusor

Objectives.The human bladder shows spontaneous autonomous activity. Detrusor overactivity could be seen as a consequence of exaggerated autonomous activity. Interstitial cells (ICs) play a potential role in coordination of autonomous activity. As it is suggested that changes in ICs coexist with detrusor overactivity (DO), we investigated possible alterations to human bladder ICs.Methods.Biopsies were obtained from 23 patients and were categorized into four groups: genuine stress incontinence (without DO) (n=5), neurogenic disease with DO (n=6), bladder outlet obstruction with DO (n=6), or idiopathic DO (n=6). Specimens were processed to investigate expression of N-cadherin and PGP9.5. N-cadherin expression was semiquantitatively analyzed and correlated to PG9.5 expression and bladder wall morphology.Results.The population of cells expressing N-cadherin is altered in the overactive detrusor, making no difference between the sources of DO. Punctate distribution of morphological changes was found and downregulation of PGP9.5 expression seemed to coexist with upregulation of N-cadherin expression in the detrusor layer.Conclusions.The population of N-cadherin+ cells of the interstitial compartment of the human bladder has the ability to proliferate. As this proliferation seems to coexist with denervation, it could be possible that a highly developed network of interstitial cells replaces the loss of innervation in overactive detrusor.

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1683 EFFECT OF DETRUSOR OVERACTIVITY INDUCED BY BLADDER OUTLET OBSTRUCTION ON INTERSTITIAL CELLS IN THE URINARY BLADDER OF RATS

The Journal of Urology ◽

10.1016/j.juro.2010.02.1508 ◽

2010 ◽

Vol 183 (4S) ◽

Author(s):

Byung Seok Oh ◽

Kim Sun-Ouck ◽

Jun Back Park ◽

Chang Min Im ◽

Kyung Jin Oh ◽

...

Keyword(s):

Urinary Bladder ◽

Detrusor Overactivity ◽

Bladder Outlet Obstruction ◽

Outlet Obstruction ◽

Interstitial Cells

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Mechanosensitivity Is a Characteristic Feature of Cultured Suburothelial Interstitial Cells of the Human Bladder

International Journal of Molecular Sciences ◽

10.3390/ijms21155474 ◽

2020 ◽

Vol 21 (15) ◽

pp. 5474

Author(s):

Jochen Neuhaus ◽

Andreas Gonsior ◽

Sheng Cheng ◽

Jens-Uwe Stolzenburg ◽

Frank Peter Berger

Keyword(s):

Mechanical Stress ◽

Single Cells ◽

Bladder Wall ◽

High Sensitivity ◽

Interstitial Cells ◽

Calcium Signal ◽

Local Pressure ◽

Human Bladder ◽

Calcium Activity

Bladder dysfunction is characterized by urgency, frequency (pollakisuria, nocturia), and dysuria and may lead to urinary incontinence. Most of these symptoms can be attributed to disturbed bladder sensitivity. There is growing evidence that, besides the urothelium, suburothelial interstitial cells (suICs) are involved in bladder afferent signal processing. The massive expansion of the bladder during the filling phase implicates mechanical stress delivered to the whole bladder wall. Little is known about the reaction of suICs upon mechanical stress. Therefore, we investigated the effects of mechanical stimulation in cultured human suICs. We used fura-2 calcium imaging as a major physiological readout. We found spontaneous intracellular calcium activity in 75 % of the cultured suICs. Defined local pressure application via a glass micropipette led to local increased calcium activity in all stimulated suICs, spreading over the whole cell. A total of 51% of the neighboring cells in a radius of up to 100 µm from the stimulated cell showed an increased activity. Hypotonic ringer and shear stress also induced calcium transients. We found an 18-times increase in syncytial activity compared to unstimulated controls, resulting in an amplification of the primary calcium signal elicited in single cells by 50%. Our results speak in favor of a high sensitivity of suICs for mechanical stress and support the view of a functional syncytium between suICs, which can amplify and distribute local stimuli. Previous studies of connexin expression in the human bladder suggest that this mechanism could also be relevant in normal and pathological function of the bladder in vivo.

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Faculty Opinions recommendation of Altered expression of calcium-activated K and Cl channels in detrusor overactivity of rats with partial bladder outlet obstruction.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1103128.559114 ◽

2008 ◽

Author(s):

Rodolfo Testa

Keyword(s):

Detrusor Overactivity ◽

Bladder Outlet Obstruction ◽

Outlet Obstruction ◽

Altered Expression ◽

Partial Bladder Outlet Obstruction ◽

Cl Channels

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THE DIAGNOSIS OF BLADDER OUTLET OBSTRUCTION IN MEN BY ULTRASOUND MEASUREMENT OF BLADDER WALL THICKNESS

The Journal of Urology ◽

10.1016/s0022-5347(01)63723-6 ◽

1998 ◽

Vol 159 (3) ◽

pp. 761-765 ◽

Cited By ~ 142

Author(s):

CARLO MANIERI ◽

SIMON ST. C. CARTER ◽

GIANFRANCO ROMANO ◽

ALBERTO TRUCCHI ◽

MARCO VALENTI ◽

...

Keyword(s):

Wall Thickness ◽

Bladder Outlet Obstruction ◽

Bladder Wall ◽

Outlet Obstruction ◽

Ultrasound Measurement ◽

Bladder Wall Thickness

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Age and Bladder Outlet Obstruction Are Independently Associated with Detrusor Overactivity in Patients with Benign Prostatic Hyperplasia

European Urology ◽

10.1016/j.eururo.2008.02.017 ◽

2008 ◽

Vol 54 (2) ◽

pp. 419-426 ◽

Cited By ~ 117

Author(s):

Matthias Oelke ◽

Joyce Baard ◽

Hessel Wijkstra ◽

Jean J. de la Rosette ◽

Udo Jonas ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Detrusor Overactivity ◽

Bladder Outlet Obstruction ◽

Outlet Obstruction ◽

Prostatic Hyperplasia

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Effects of Nitric Oxide Synthases on Detrusor Overactivity after Removal of Bladder Outlet Obstruction in Rats

Urologia Internationalis ◽

10.1159/000137650 ◽

2008 ◽

Vol 81 (1) ◽

pp. 107-112 ◽

Cited By ~ 5

Author(s):

H.S. Kim ◽

J.C. Kim ◽

M.S. Choo

Keyword(s):

Nitric Oxide ◽

Detrusor Overactivity ◽

Bladder Outlet Obstruction ◽

Outlet Obstruction ◽

Nitric Oxide Synthases

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SIGNIFICANCE OF NON-INVASIVE SONO-MORPHOLOGICAL MARKERS IN THE EVALUATION OF BLADDER OUTLET OBSTRUCTION AND ACUTE URINARY RETENTION SECONDARY TO BENIGN PROSTATIC HYPERPLASIA

10.36106/ijsr/5423553 ◽

2021 ◽

pp. 5-9

Author(s):

Prasenjit Bhowmik ◽

Soumendranath Mandal ◽

Gaurav Sharma ◽

Bandhan Bahal ◽

Prashant Gupta ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Diagnostic Accuracy ◽

Urinary Retention ◽

Bladder Outlet Obstruction ◽

Acute Urinary Retention ◽

Bladder Wall ◽

Outlet Obstruction ◽

Threshold Value ◽

Prostatic Hyperplasia ◽

Control Group

Objectives: To dene the diagnostic accuracy of intravesical protrusion of prostate (IPP), bladder wall thickness (BWT) and prostate volume (PV) in diagnosis of bladder outlet obstruction (BOO) and prediction of future acute urinary retention (AUR). A prospective Materials and methods: study of 127 patients, presenting with lower urinary tract symptoms suggestive of benign prostatic hyperplasia (BPH) were enrolled with 50 patients among them manifesting AUR. 35 normal persons were selected as control group. After inclusion, all patients underwent transabdominal ultrasound and pressure ow urodynamic study (UDS). UDS parameter, BOO index (BOOI) were used as a reference standard for stratifying the patients into two groups, BOO and non-BOO. The sono-morphological parameters, IPP, BWT and PV were used to compare between these two groups with calculating the diagnostic accuracy for each individual index. IPP, BWT Results: and PV had statistically signicant difference between group 1 (BOOI < 40) and 2 ( BOOI > 40) with strong correlation with BOOI. The Pearson's correlation coefcient (r) for IPP, BWT and PV were 0.762, 0.702 and 0.660 respectively. The AUC for IPP, BWT and PV were 0.824, 0.786 and 0.650 with highest accuracy for IPP (79.2%) at cutoff value of 7 mm. Using the same threshold value, IPP had higher statistical difference than BWT in predicting AUR with similar diagnostic accuracy of IPP and BWT together. IPP and BWT in conjunction with PV in place Conclusions: of UDS had good clinical utility in diagnosis of BOO due to BPH and future AUR prediction.

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