scholarly journals Citric Acid Production by Aspergillus niger Cultivated on Parkia biglobosa Fruit Pulp

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Helen Shnada Auta ◽  
Khadijat Toyin Abidoye ◽  
Hauwa Tahir ◽  
Aliyu Dabai Ibrahim ◽  
Sesan Abiodun Aransiola
Keyword(s):  
Citric Acid ◽  
Acid Production ◽  
Incubation Temperature ◽  
Sugar Content ◽  
Inoculum Size ◽  
Reducing Sugar ◽  
Citric Acid Production ◽  
Parkia Biglobosa ◽  
Acid Yield ◽  
Fermentation Period

The study was conducted to investigate the potential of Parkia biglobosa fruit pulp as substrate for citric acid production by Aspergillus niger. Reducing sugar was estimated by 3,5-dinitrosalicylic acid and citric acid was estimated spectrophotometrically using pyridine-acetic anhydride methods. The studies revealed that production parameters (pH, inoculum size, substrate concentration, incubation temperature, and fermentation period) had profound effect on the amount of citric acid produced. The maximum yield was obtained at the pH of 2 with citric acid of 1.15 g/L and reducing sugar content of 0.541 mMol−1, 3% vegetative inoculum size with citric acid yield of 0.53 g/L and reducing sugar content of 8.87 mMol−1, 2% of the substrate concentration with citric acid yield of 0.83 g/L and reducing sugar content of 9.36 mMol−1, incubation temperature of 55°C with citric acid yield of 0.62 g/L and reducing sugar content of 8.37 mMol−1, and fermentation period of 5 days with citric acid yield of 0.61 g/L and reducing sugar content of 3.70 mMol−1. The results of this study are encouraging and suggest that Parkia biglobosa pulp can be harnessed at low concentration for large scale citric acid production.

EFFECT OF FERROCYANIDE ON GROWTH AND CITRIC ACID PRODUCTION BY ASPERGILLUS NIGER

1966 ◽  
Vol 12 (5) ◽  
pp. 901-907 ◽  
Author(s):  
H. Horitsu ◽  
D. S. Clark
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Krebs Cycle ◽  
Acid Formation ◽  
Citric Acid Production ◽  
Resting Cells ◽  
Acid Yield ◽  
Beet Molasses ◽  
Inhibition Of Growth

Ferrocyanide at concentrations of less than 30 p.p.m. (the amount tolerated in citric acid fermentation of beet molasses) had no measurable effect on citric acid production or on the oxidation of glucose or Krebs cycle compounds by resting cells of Aspergillus niger or on the growth rate of this organism during submerged fermentation of beet molasses. Concentrations above 30 p.p.m., however, stimulated citric acid formation in resting cells, but markedly inhibited cell development in growing cells. This inhibition of growth was the main cause of the detrimental effect of high concentrations of ferrocyanide on citric acid formation in molasses; good growth throughout the fermentation was essential to high acid yield, inhibition of growth could be released at any time during the fermentation by addition of sufficient ZnSO4 to reduce the ferrocyanide content to below 30 p.p.m. No evidence that ferrocyanide favors citric acid accumulation by blocking a reaction in the Krebs cycle was found.

scholarly journals Improving Citric acid Production of an Industrial Aspergillus Niger CGMCC 10142: Identification and Overexpression of a High-affinity Glucose Transporter with Different Promoters

2020 ◽  
Author(s):  
Xianli Xue ◽  
Futi Bi ◽  
Boya Liu ◽  
Jie Li ◽  
Lan Zhang ◽  
...  
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Glucose Transporter ◽  
Citrate Synthase ◽  
Sugar Content ◽  
Citric Acid Production ◽  
Acid Fermentation ◽  
Total Sugar Content ◽  
High Affinity

Abstract Background: The transporter of glucose plays an important role in the fermentation of citric acid. In this research, a high-affinity glucose transporter (HGT1) was identified and overexpressed in industrial strain A. niger CGMCC 10142. The overexpressed-HGT1 strains were obtained to verify the glucose transporter functions with different promoters PglaA and Paox1.Result: The sugar contents and citric acid production during fermentation were tracked. As speculated, the HGT1-overexpressed strains showed higher citric acid production and lower residual sugar contents. The best-performing strain on the fermentation is A. niger 20-15 transformant of which the total sugar content and residual reducing sugar both were reduced by 31% and 44.9% comparing to A. niger CGMCC 10142, and the final citric acid production was correspondingly increased to 174.1 g/L comparing to A. niger CGMCC 10142 with 162.3 g/L. Besides HGT1, the transcription levels of related genes as citrate synthase and glucokinase in transformants were also significantly higher than A. niger CGMCC 10142.Conclusion: The results indicate that HGT1 understandably alleviates the metabolic restriction caused by insufficient sugar transporters and finally improves the utilization of sugar. This study confirms the important role of glucose transporter HGT1 in the citric acid fermentation process of Aspergillus niger.

scholarly journals Improving citric acid production of an industrial Aspergillus niger CGMCC 10142: identification and overexpression of a high-affinity glucose transporter with different promoters

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Xianli Xue ◽  
Futi Bi ◽  
Boya Liu ◽  
Jie Li ◽  
Lan Zhang ◽  
...  
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Glucose Transporter ◽  
Citrate Synthase ◽  
Sugar Content ◽  
Sugar Transport ◽  
Citric Acid Production ◽  
Utilization Rate ◽  
High Affinity

Abstract Background Glucose transporters play an important role in the fermentation of citric acid. In this study, a high-affinity glucose transporter (HGT1) was identified and overexpressed in the industrial strain A. niger CGMCC 10142. HGT1-overexpressing strains using the PglaA and Paox1 promoters were constructed to verify the glucose transporter functions. Result As hypothesized, the HGT1-overexpressing strains showed higher citric acid production and lower residual sugar contents. The best-performing strain A. niger 20-15 exhibited a reduction of the total sugar content and residual reducing sugars by 16.5 and 44.7%, while the final citric acid production was significantly increased to 174.1 g/L, representing a 7.3% increase compared to A. niger CGMCC 10142. Measurement of the mRNA expression levels of relevant genes at different time-points during the fermentation indicated that in addition to HGT1, citrate synthase and glucokinase were also expressed at higher levels in the overexpression strains. Conclusion The results indicate that HGT1 overexpression resolved the metabolic bottleneck caused by insufficient sugar transport and thereby improved the sugar utilization rate. This study demonstrates the usefulness of the high-affinity glucose transporter HGT1 for improving the citric acid fermentation process of Aspergillus niger CGMCC 10142.

scholarly journals Biological production of citric acid in submerged culture of Aspergillus niger using cassava pulp wastes

2021 ◽  
Vol 3 (3) ◽  
pp. 69-74
Author(s):  
Ifenayi Boniface Ezea ◽  
Emmanuel Ezaka ◽  
Joy Onyekachi Iwuagwu ◽  
Calista Odinachi Itubochi
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Acid Concentration ◽  
Soybean Meal ◽  
Acid Production ◽  
Submerged Culture ◽  
Incubation Temperature ◽  
Citric Acid Production ◽  
Citric Acid Concentration ◽  
Cassava Pulp

Utilization of cassava pulp wastes for citric acid production was investigated using Aspergillus niger in a submerged culture. A series of experiments were designed on various fermentation parameters to establish the optimal conditions for citric acid production from cassava pulp. This study revealed that production parameters such as cassava pulp concentration, initial pH, incubation temperature, agitation, and nitrogen source and fermentation period had effect on the amount of citric acid produced from cassava pulp. Citric acid concentration increased as the concentration of cassava pulp increases up to 20% with maximum citric acid concentration of 14.9 ± 0.413 g/l after 120 hours of fermentation. pH 5.5 was the optimum with maximum  citric acid concentration of 16.8 ± 0.23 g/l after 120 hours of fermentation. Incubation temperature at 300 C was the optimum, with citric acid concentration of 19.15 ± 0.43 g/l. Increased in agitation speed from 100 to 225 rpm gave the maximum citric acid concentration of 25.2 ± 0.32 g/l after 120 hours of fermentation. Soybean meal supplementation was the best maximum citric acid concentration of 28.2 ± 0.51 g/l. Evaluating the effect of different concentration of soybean meal shows that 0.3 %  supplementation was the optimum with maximum concentration of 31.2 ±0.35g/l from cassava pulp after 120 hours of fermentation. The result suggested that citric acid can be accumulated using cassava pulp by Aspergillus niger in submerged culture during fermentation. Cassava pulp if well harnessed can be used for large scale citric acid production.

Effect of Various Parameters on Citric Acid Production using Different Substrates

2012 ◽  
Vol 3 (7) ◽  
pp. 1-3
Author(s):  
Pawar V.A Pawar V.A ◽  
◽  
Pawar P.R Pawar P.R
Keyword(s):  
Citric Acid ◽  
Acid Production ◽  
Citric Acid Production

scholarly journals The Effect of Lipids on Citric Acid Production by an Aspergillus niger Mutant

1963 ◽  
Vol 30 (3) ◽  
pp. 365-379 ◽  
Author(s):  
N. F. MILLIS ◽  
B. H. TRUMPY ◽  
B. M. PALMER
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Citric Acid Production

Optimization of Trace Metals Concentration on Citric Acid Production by Aspergillus niger NRRL 2001

2007 ◽  
Vol 1 (3) ◽  
pp. 246-253 ◽  
Author(s):  
A. A. Guilherme ◽  
G. A. S. Pinto ◽  
S. Rodrigues
Keyword(s):  
Citric Acid ◽  
Trace Metals ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Citric Acid Production
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