Blood Count and Serum Biochemistry Profile in West African Dwarf Goats Fed Ensiled Mixtures of Cocoa Pod, Cassava Pulp and Acacia Leaf

This study investigated the effects of feeding ensiled graded combinations of cocoa pod, cassava pulp and Acacia leaf on haematological and serum biochemical of West African Dwarf goats. In the bid to control environmental pollution, the wastes of cocoa and cassava were applied as alternative feed ingredients for the goat in spite of its low nutrients’ quality and digestibility. A total of Twenty-eight (28) WAD goats (bucks), aged 4 to 5 months with average body weight of 7.00±0.2kg was used in a completely randomized design for a 45 days study to evaluate the blood profiles of goats fed diet combinations (0:60:40) to (60:0:40). The results revealed significant differences in some of the blood parameters measured among the treatments. The red blood cell, packed cell volume and haemoglobin ranged from 4.90-7.87x106/ul, 19.25-26.83% and 7.33-11.15g/L respectively. The concentrations of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration varied significantly (p < 0.05), white blood cell (5.57-9.75x109L) were comparable and lymphocytes (%) ranged from 43.85-55.67. For serum biochemistry, values obtained for total protein and albumin with sodium followed the same trend while cholesterol, urea, creatinine and glucose followed the same trend. Alanine amino transferase, Aspartate aminotransferase and Alkaline phosphatase varied significantly (p<0.05) among the treatment groups. Since the parameters measured were within the normal range for goats’ blood profile, combinations of cocoa pod, cassava pulp with acacia leaves in the ratio of 0:60:40 to 20:40:40 as browse plants do not have any deleterious effect on the health of the animals.

SYNTHESIS L-LACTIC ACID FROM FERMENTATION OF CASSAVA PULP BY USING TEMPEH INOCULUM

SYNTHESIS L-LACTIC ACID FROM FERMENTATION OF CASSAVA PULP BY USING TEMPEH INOCULUM. This study used cassava waste pulp as a fermentation substrate to produce lactic acid using a tempeh inoculum. Tempeh inoculum is a mixed culture of Rhizopus with Rhizopus oligosporus as the primary fungus. Lactic acid is an organic acid most widely used in the food, pharmaceutical, cosmetic and chemical industries. One of the important uses of lactic acid is as a raw material for producing Polylactic Acid (PLA) biopolymers, namely polymers that can decompose naturally in a relatively fast time. The analysis was performed using the Response Surface Methodology (RSM) method and the Box Behnken Design (BBD) experimental design with substrate concentration parameters, inoculum concentration, and incubation time on lactic acid. The fermentation process is carried out using a flask shaker at a temperature of 30 ºC, pH 6.0, and a rotational speed of 150 rpm. The optimum yield for lactic acid is 6.65 g/L. It was acquired at substrate 20 g/L, inoculum concentration 0.30 % (w/v) at an incubation time of 72 hours.

Bio-Methane Potential (BMP) of Cassava Pulp Waste and Effect of Alkaline Pre-Treatment

Cassava starch processing industry produces cassava pulp as a by-product or waste. In the well-known Duong Lieu village, this waste is released in surrounding environment without treatment causing serious environmental problems. The study aimed to (1) determine the Biomethane Potential (BMP) of the waste and to (2) find out if alkaline pre-treatment would improve it. Different cassava pulp samples were going through BMP test: untreated sample; pre-treated samples at different NaOH doses of 2, 6, 8 wt.% (dry weight-based) and pre-treated samples at different NaHCO3 doses of 2, 4, 6, 8 wt.% (dry weight based). BMP assays were conducted in 590mL bottles at 37oC for 40 days. As the result, BMP of the untreated waste was 281 NmLCH4/gVS and alkaline pretreatment increased BMP of the waste up to 479 mLCH4/gVS by treatment with NaOH 6 wt.% and 450 mLCH4/gVS by treatment with NaHCO3 6 wt.%. In addition, there was a significant reduction of lignin content of the substrate after alkaline pre-treatment. The results show that cassava pulp waste has moderate potential for biogas recovery. In addition, alkaline pre-treatment by either NaOH or NaHCO3 would significantly improve its BMP, possibly thanks to the reduction of lignin content.

Biological production of citric acid in submerged culture of Aspergillus niger using cassava pulp wastes

Utilization of cassava pulp wastes for citric acid production was investigated using Aspergillus niger in a submerged culture. A series of experiments were designed on various fermentation parameters to establish the optimal conditions for citric acid production from cassava pulp. This study revealed that production parameters such as cassava pulp concentration, initial pH, incubation temperature, agitation, and nitrogen source and fermentation period had effect on the amount of citric acid produced from cassava pulp. Citric acid concentration increased as the concentration of cassava pulp increases up to 20% with maximum citric acid concentration of 14.9 ± 0.413 g/l after 120 hours of fermentation. pH 5.5 was the optimum with maximum citric acid concentration of 16.8 ± 0.23 g/l after 120 hours of fermentation. Incubation temperature at 300 C was the optimum, with citric acid concentration of 19.15 ± 0.43 g/l. Increased in agitation speed from 100 to 225 rpm gave the maximum citric acid concentration of 25.2 ± 0.32 g/l after 120 hours of fermentation. Soybean meal supplementation was the best maximum citric acid concentration of 28.2 ± 0.51 g/l. Evaluating the effect of different concentration of soybean meal shows that 0.3 % supplementation was the optimum with maximum concentration of 31.2 ±0.35g/l from cassava pulp after 120 hours of fermentation. The result suggested that citric acid can be accumulated using cassava pulp by Aspergillus niger in submerged culture during fermentation. Cassava pulp if well harnessed can be used for large scale citric acid production.

Variation in Toxicity and Physicochemical Parameters of Cassava Pulp (Manihot esculenta) during Storage

Background: A staple for about 800 million people in tropical countries, cassava contains a high content of hydrocyanic acid making it toxic. Objective: This study was proposed to test the conservation effectiveness on tuber toxicity. Materials and Methods: Tests were carried out on the varieties commonly known as Bonoua and Yacé. The conservation parameters studied were: the level of hydrocyanic acid, moisture content and hardness. The cassava tubers were stored at room temperature. The samples for analysis were taken on the pulp every 24 hours. Results: Results showed that hydrocyanic acid levels reached their minimum levels between 96 and 120 hours of storage with 5.87 ± 0.46 and 5.66 ± 0.50 mg/kg in Bonoua, then 9.53 ± 0.78 and 9.85 ± 0.93 mg/kg in IAC. Water levels are positively correlated with those of hydrocyanic acid in both cassava types. Proteins reach reveal in their maximum concentrations between 48 and 72 hours of storage (respectively to then drop. The ash and fat contents as well as the total carbohydrate contents generally decrease during storage. However, an increase is observed from 120 hours of storage, at the level of carbohydrate concentrations. Conclusion: For a non-toxic cassava pulp, a storage period of at least 48 hours at the Bonoua and 96 hours at the IAC would be advisable with however a lower biochemical quality.

Manipulation of In Vitro Ruminal Fermentation and Feed Digestibility as Influenced by Yeast Waste-Treated Cassava Pulp Substitute Soybean Meal and Different Roughage to Concentrate Ratio

Cassava pulp (CS) is high in fiber and low in protein; hence, improving the nutritive value of CS is required to increase its contribution to enhancing ruminant production. The present work hypothesized that CS quality could be enhanced by fermentation with yeast waste (YW), which can be used to replace soybean meal (SBM), as well as lead to improved feed utilization in ruminants. Thus, evaluation of in vitro ruminal fermentation and feed digestibility, as influenced by YW-treated CS and different roughage (R) to concentrate (C) ratios, was elucidated. The design of the experiment was a 5 × 3 factorial arrangement in a completely randomized design. Each treatment contained three replications and three runs. The first factor was replacing SBM with CS fermented with YW (CSYW) in a concentrate ratio at 100:0, 75:25, 50:50, 25:75, and 0:100, respectively. The second factor was R:C ratios at 70:30, 50:50, and 30:70. The level of CSYW showed significantly higher (p < 0.01) gas production from the insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h than the control group (p < 0.05). There were no interactions among the CSYW and R:C ratio on the in vitro digestibility (p > 0.05). Furthermore, increasing the amount of CSYW to replace SBM up to 75% had no negative effect on in vitro neutral detergent fiber degradability (IVNDFD) (p > 0.05) while replacing CSWY at 100% could reduce IVNDFD (p > 0.05). The bacterial population in the rumen was reduced by 25.05% when CSYW completely replaced SBM (p < 0.05); however, 75% of CSWY in the diet did not change the bacterial population (p > 0.05). The concentration of propionate (C3) decreased upon an increase in the CSYW level, which was lowest with the replacement of SBM by CSYW up to 75%. However, various R:C ratios did not influence total volatile fatty acids (VFAs), and the proportion of VFAs (p > 0.05), except the concentration of C3, increased when the proportion of a concentrate diet increased (p < 0.05). In conclusion, CSYW could be utilized as a partial replacement for SBM in concentrate diets up to 75% without affecting gas kinetics, ruminal parameters, or in vitro digestibility.

ANALISIS NILAI TAMBAH BERBAGAI PRODUK OLAHAN INDUSTRI RUMAH TANGGA BERBASIS BAHAN BAKU UBI KAYU DI KECAMATAN GERUNG KABUPATEN LOMBOK BARAT

This study aims to: (1) analyze the added value of processing business based on cassava raw material in Gerung District, West Lombok Regency, (2) analyze the benefit of processing business based on cassava raw material in Gerung District, West Lombok Regency, and (3) analyze the inhibiting factors of processing business based on cassava raw materials in Gerung District, West Lombok Regency. This research uses a descriptive method. The types of data used are quantitative and qualitative data. Sources of data used are primary data and secondary data. Data analysis using Hayami Method and profit analysis. The results showed that the added value was Rp. 9.571/kg (with a ratio of 67.79% and a profit rate of 80.33%) for cassava tape agroindustry, Rp. 18,191/kg (with a ratio of 71.34% and a profit rate of 88.20%) for cassava chips agroindustry, and Rp. 5.558/kg (with a ratio of 28% and a profit rate of 64.02%) for cassava pulp agroindustry. Profit of Rp. 384,782/production process and Rp. 10,389,114/month obtained from cassava tape agroindustry, Rp. 741,224/production process and Rp. 8,894,688/month obtained from cassava chips agroindustry, and Rp. 53,377/production process and Rp. 1,281,048/month obtained from cassava pulp agroindustry; and The inhibiting factors faced by processed entrepreneurs based on cassava raw materials in Gerung District, West Lombok Regency are the lack of raw material availability and limited working capital.