scholarly journals The Child as a Surrogate for Diagnosis of Lupus in the Mother

2017 ◽  
Vol 2017 ◽  
pp. 1-2
Author(s):  
Olufemi O. Adelowo ◽  
Kenneth A. Ohagwu ◽  
Ejiehi E. Aigbokhan ◽  
Richard O. Akintayo
Keyword(s):  
Lupus Erythematosus ◽  
Complete Heart Block ◽  
Male Infant ◽  
Antenatal Clinic ◽  
Clinic Visit ◽  
Nuclear Antigen ◽  
Neonatal Lupus Erythematosus ◽  
Extractable Nuclear Antigen ◽  
Erythrocyte Sedimentation ◽  
Pediatric Echocardiography

Introduction. Neonatal lupus erythematosus (NLE) is an acquired disease of the newborn caused by transplacental transfer of maternal anti-Ro/SSA, anti-La/SSB, and infrequently anti-U1 RNP antibodies. Methodology. This is a case report of a male infant delivered via Caesarean section at 36-week gestation following detection of fetal bradycardia during routine antenatal clinic visit. Results. The mother was seropositive for antinuclear antibody (ANA) and anti-Ro/SSA and had elevated erythrocyte sedimentation rate. The baby was positive for ANA, extractable nuclear antigen (ENA), and anti-Ro/SSA. Pediatric echocardiography was abnormal and electrocardiography confirmed complete heart block.

scholarly journals Congenital and neonatal lupus erythematosus: two case reports

2012 ◽  
Vol 87 (4) ◽  
pp. 625-628
Author(s):  
Marcos Noronha Frey ◽  
Ana Elisa Empinotti Ioppi ◽  
Gabriela Czarnobay Garbin ◽  
Roque Domingos Furian ◽  
Ana Elisa Kiszewski Bau
Keyword(s):  
Lupus Erythematosus ◽  
Antinuclear Antibodies ◽  
Case Reports ◽  
Nuclear Antigen ◽  
Neonatal Lupus ◽  
Cutaneous Lesions ◽  
Neonatal Lupus Erythematosus ◽  
Extractable Nuclear Antigen ◽  
The Moment ◽  
Congenital Variant

Neonatal lupus erythematosus is an autoimmune disease produced by the passage of maternal antinuclear antibodies and extractable nuclear antigen antibodies through the placenta. At the moment of the diagnosis, the mothers are asymptomatic in 40 to 60% of cases. The most common manifestations are cutaneous lesions and congenital heart block. The cutaneous findings are variable and usually begin within the first weeks or months of life. Congenital lupus erythematosus is a congenital variant of neonatal lupus erythematosus. We present one case of congenital lupus erythematosus and one case of neonatal lupus erythematous, showing the variability of this disease.

scholarly journals Survivorship in systemic lupus erythematosus. effect of antibody to extractable nuclear antigen

1981 ◽  
Vol 24 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Marc C Hochberg ◽  
Carole A Dorsch ◽  
Edward J Feinglass ◽  
Mary Betty Stevens
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Nuclear Antigen ◽  
Systemic Lupus ◽  
Extractable Nuclear Antigen

scholarly journals Association of Immunofluorescence pattern of Antinuclear Antibody with Specific Autoantibodies in the Bangladeshi Population

2015 ◽  
Vol 40 (2) ◽  
pp. 74-78 ◽  
Author(s):  
S Sharmin ◽  
S Ahmed ◽  
A Abu Saleh ◽  
F Rahman ◽  
MR Choudhury ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Research Work ◽  
Connective Tissue Disorder ◽  
Nuclear Antigen ◽  
Dot Blot ◽  
Serum Samples ◽  
Speckled Pattern ◽  
Extractable Nuclear Antigen

Antinuclear antibody (ANA) is useful in the diagnosis of connective tissue disorder (CTD). Association of specific autoantibodies with the immunofluorescence pattern of ANA in CTD, noted in western literature has been considered as reference in all over the world. However, in Bangladesh no such research work or data correlating the autoantibodies and their ANA patterns is found. Objective of the study was to identify an association between immunofluorescence patterns of antinuclear antibody on HEp-2 cell and more specific antinuclear reactivities (e.g. anti-dsDNA and anti-extractable nuclear antigen) in the serum samples of CTD patients. Serum samples of 152 CTD patients (Systemic lupus erythematosus, Rhumatoid arthritis, Sjogren´s syndrome, Systemic sclerosis, Polymyositis, Mixed connective tissue disease) were diagnosed clinically, attending at Bangabandhu Sheikh Mujib Medical University (BSMMU) during the study period of January, 2010 to December, 2010. Samples were subjected for ANA testing by Indirect Immunofluorescence (IIF) on HEp-2 cell (ALPHADIA) in dilution of 1:40, anti-dsDNA by ELISA and anti- extractable nuclear antigen (anti-ENA) by Dot Immunoblot. Dot blot strips were tested for anti-Sm, anti-RNP, anti-SSA/Ro, anti-SSB/La, anti-Scl-70 and anti-Jo-1. Out of 152 patients 110 (72.3%) cases were ANA positive by IIF on HEp-2 cell. ANA positive sera exhibited four fluorescence patterns such as speckled (50.8%), peripheral (21.6%) ,homogenous (18.1%) and nucleolar pattern (9%). Peripheral pattern and homogenous pattern was predominantly associated with anti-dsDNA (p<0.05). Speckled pattern was significantly associated with anti-ENA (p<0.05).The most commonly identified antinuclear autoreactivity was directed towards anti-RNP (25.7%) then anti-Scl-70 (20%), anti-SSA (14.2%) and anti-SSB (5.7%). Multiple anti-ENA reactivities were identified in 34.28% cases. Peripheral and homogenous pattern is strongly associated with anti-dsDNA and speckled pattern may predict anti-ENA (specially ribonucleoprotiens). As a definite correlation between the ANA patterns and the group of antibodies was detected by dot immunoblot, one could predict presence of certain specific auto antibodies for a particular ANA pattern identified. This may restrict on the cost of laboratory investigations in a developing country like Bangladesh. Thus, ANA-IIF method may reduce the expense of detailed immunological work-up with minimal loss in diagnostic accuracy.Bangladesh Med Res Counc Bull 2014; 40 (2): 74-78

scholarly journals Evaluation of an Automated Screening Assay, Compared to Indirect Immunofluorescence, an Extractable Nuclear Antigen Assay, and a Line Immunoassay in a Large Cohort of Asian Patients with Antinuclear Antibody-Associated Rheumatoid Diseases: A Multicenter Retrospective Study

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Seri Jeong ◽  
Hyunyong Hwang ◽  
Juhye Roh ◽  
Jung Eun Shim ◽  
Jinmi Kim ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Connective Tissue Disease ◽  
Indirect Immunofluorescence ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Nuclear Antigen ◽  
University Hospitals ◽  
Screening Assay ◽  
Extractable Nuclear Antigen ◽  
Automated Screening

We assessed the diagnostic utility of the connective tissue disease (CTD) screen as an automated screening test, in comparison with the indirect immunofluorescence (IIF), EliA extractable nuclear antigen (ENA), and line immunoassay (LIA) for patients with antinuclear antibody- (ANA-) associated rheumatoid disease (AARD). A total of 1115 serum samples from two university hospitals were assayed using these four autoantibody-based methods. The AARD group consisted of patients with systemic lupus erythematosus (SLE), systemic sclerosis (SSc), Sjögren’s syndrome (SS), and mixed connective tissue disease (MCTD). The qualitative results of all four autoantibody assays showed a significant association with AARDs, compared to controls (P<0.0001 for all). The areas under the receiver operating characteristic curves (ROC-AUCs) of the CTD screen for differentiating total AARDs, SLE, SSc, SS, and MCTD from controls were 0.89, 0.93, 0.73, 0.93, and 0.95, respectively. The ROC-AUCs of combination testing with LIA were slightly higher in patients with AARDs (0.92) than those of CTD screen alone. Multivariate analysis indicated that all four autoantibody assays could independently predict AARDs. CTD screening alone and in combination with IIF, EliA ENA, and LIA are potentially valuable diagnostic approaches for predicting AARDs. Combining CTD screen with LIA might be effective for AARD patients.

scholarly journals Immunofluorescence pattern of antinuclear antibody and its association with autoantibody profile in systemic lupus erythematosus

2016 ◽  
Vol 6 (2) ◽  
pp. 141
Author(s):  
Sadia Sharmin ◽  
Sharmeen Ahmed ◽  
Humayun Sattar ◽  
Md. Ruhul Amin Miah ◽  
Minhaz Rahim Chowdhury ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Research Work ◽  
Nuclear Antigen ◽  
Dot Blot ◽  
Systemic Lupus ◽  
Serum Samples ◽  
Speckled Pattern ◽  
Extractable Nuclear Antigen

<p><strong>Background:</strong> Antinuclear antibody (ANA) is useful in the diagnosis of systemic lupus erythematosus (SLE). Association of specific autoantibodies with the immunofluorescence pattern of ANA in SLE as noted in Western literature has been taken as reference in all over the world. However, in Bangladesh such research work or data correlating the autoantibodies and their ANA patterns is inadequate. <strong></strong></p><p><strong>Objective:</strong> To identify an association between immunofluorescence patterns of antinuclear antibody on HEp-2 cell and more specific antinuclear reactivities (e.g. anti-dsDNA and anti-extractable nuclear antigen) in the serum samples of SLE patients.</p><p><strong>Methods:</strong> Serum samples of 37 SLE patients who were diagnosed by ARA (American Rheumatism Association) classification criteria and laboratory tests, attending at lupus clinic of Bangabandhu Sheikh Mujib Medical University (BSMMU) during the study period of six months were subjected for ANA testing by Indirect Imrnunofluorescence (IIF) on HEp-2 cell, anti-dsDNA by ELISA and anti- extractable nuclear antigen (anti-ENA) by Dot Immunoblot. Dot blot strips were tested for anti-Sm, anti-RNP, anti-SSA/Ro, and anti-SSB/La. <strong></strong></p><p><strong>Results:</strong> Out of 37 SLE patients 32 (86.5%) cases were ANA positive by IIF on HEp-2 cell. ANA positive sera exhibited three fluorescence patterns such as speckled (43.7%), peripheral (34.3%) and homogenous pattern (21.8%). Peripheral pattern (100%) was strongly associated with anti-dsDNA (p&lt;0.05) and homogenous pattern (85.7%) was also predominantly associated with anti-dsDNA (p&lt;0.05). Speckled pattern (85.6%) was significantly associated with anti-ENA (p&lt;0.05). Anti-dsDNA was positive in 75% of SLE cases and majority (45.8%) of which showed peripheral pattern whereas anti-ENA was positive in 48.6% cases and majority (70.5%) of which showed speckled pattern. The most commonly identified antinuclear autoreactivity was directed towards anti-RNP (22.2%) then anti-Sm (16.6%), anti-SSA (16.6%) and anti-SSB (11.1 %). Multiple anti-ENA reactivities were identified in 33.3% cases. <strong></strong></p><p><strong>Conclusion:</strong> Peripheral and homogenous pattern is strongly associated with anti-dsDNA therefore may be predicted that patients have active SLE and speckled pattern may predict anti-ENA (specially ribonucleoprotiens). Thus, ANA-IIF method may suffice and probably reduce the expense of detailed immunological work-up with minimal loss in diagnostic accuracy.</p>

Neonatal lupus erythematosus complicated by improved congenital complete heart block

2013 ◽  
Vol 55 (4) ◽  
pp. 521-524
Author(s):  
Tetsuya Isayama ◽  
Noboru Inamura ◽  
Nobuko Shiono ◽  
Hiroyuki Kitajima
Keyword(s):  
Lupus Erythematosus ◽  
Heart Block ◽  
Complete Heart Block ◽  
Neonatal Lupus ◽  
Neonatal Lupus Erythematosus ◽  
Congenital Complete Heart Block

Effectiveness of different methods for anti-Sm antibody identification. A multicentre study

2005 ◽  
Vol 43 (7) ◽  
Author(s):  
María Jesús Llorente ◽  
Juana Jiménez ◽  
Concepción González ◽  
Inmaculada Alarcón ◽  
Monserrat Alsina ◽  
...  
Keyword(s):  
Lupus Erythematosus ◽  
Nuclear Antigen ◽  
Dot Blot ◽  
Likelihood Ratios ◽  
Double Immunodiffusion ◽  
Serum Samples ◽  
Control Programs ◽  
Extractable Nuclear Antigen

AbstractMethods for the measurement of autoantibodies frequently provide controversial results. The objective of the present study was to evaluate the performance of Spanish Clinical Laboratories in the measurement of anti-Sm antibodies. A total of 23 laboratories participated, analysing 30 serum samples from patients with systemic lupus erythematosus and other autoimmune and non-autoimmune diseases. The laboratories used four extractable nuclear antigen screens, eight enzyme-linked immunosorbent assays (ELISAs) specific for anti-Sm, one line-blot, one dot-blot and one double immunodiffusion assay, from 15 different manufacturers. A total of 871 results were obtained. In general, very good sensitivity was obtained (95–100%), but specificity was moderate (52–86%) and must be improved. Most ELISAs and the line-blot were valid assays for anti-Sm detection and could serve as tests both for analysis and/or confirmation. The likelihood ratios indicated that both methods can be considered very useful or useful for the determination of anti-Sm antibodies. Nevertheless, the analytical quality of the methods for the measurement of anti-Sm antibodies could probably be improved by standardisation of the methods and the participation of laboratories in external quality control programs.

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