Expression of Progenitor Cell Markers in the Glial-Like Cells of Epiretinal Membranes of Different Origins

Purpose. To investigate the expression of progenitor cell markers (Sox2, Nestin, and Pax2) in idiopathic epiretinal membranes (iERMs) and nonidiopathic epiretinal membranes (niERMs) in relation to glial cell marker expression. Methods. ERMs were obtained from patients with iERMs and niERMs of different origins: proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and uveitis. The membranes were studied by flat-mount or sectional immunohistochemistry for expression of progenitor cell markers as well as glial (GFAP) and proliferation (Ki-67) markers. Results. Cells in the ERMs express strong GFAP, with strong Pax2 expression in the cell nuclei. Some of the GFAP-positive glial cells in all epiretinal membrane types colocalized with Sox2, Pax2, and Nestin. NiERMs are much more cellular than iERMs. Glial cells are more densely packed in all analyzed niERMs, whereas glial cells with long branches are found in the internal limiting membrane parts and the iERMs, which appear to form a local network by their processes. Conclusion. The GFAP-positive glial cells in ERMs are not pure glial cells, and some of them express progenitor cell markers, which indicate that these cells may have potential for self-renewal and differentiation into more glial or neuroglial type of cells.

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Effect of fractionated and hyperfractionated ionizing irradiation on inflammatory and neural progenitor cell markers in murine hippocampal tissue slices

10.1055/s-0037-1607401 ◽

2017 ◽

Author(s):

A Glasow ◽

E Kendzia ◽

I Patties ◽

S Kallendrusch ◽

RD Kortmann

Keyword(s):

Progenitor Cell ◽

Neural Progenitor Cell ◽

Neural Progenitor ◽

Ionizing Irradiation ◽

Tissue Slices ◽

Cell Markers ◽

Hippocampal Tissue

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Faculty Opinions recommendation of Identification of intramural epithelial networks linked to peribiliary glands that express progenitor cell markers and proliferate after injury in mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718012985.793484945 ◽

2013 ◽

Author(s):

Bruno Stieger

Keyword(s):

Progenitor Cell ◽

Cell Markers ◽

Peribiliary Glands

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Diagnostic utility of neural stem and progenitor cell markers nestin and SOX2 in distinguishing nodal melanocytic nevi from metastatic melanomas

Modern Pathology ◽

10.1038/modpathol.2012.132 ◽

2012 ◽

Vol 26 (1) ◽

pp. 44-53 ◽

Cited By ~ 27

Author(s):

Pei-Ling Chen ◽

Wei-Shen Chen ◽

Jianping Li ◽

Anne C Lind ◽

Dongsi Lu

Keyword(s):

Progenitor Cell ◽

Diagnostic Utility ◽

Cell Markers ◽

Melanocytic Nevi

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MRI features of hepatocellular carcinoma expressing progenitor cell markers

Liver International ◽

10.1111/j.1478-3231.2011.02640.x ◽

2011 ◽

pp. n/a-n/a

Author(s):

Hyeon Tae Jeong ◽

Myeong-Jin Kim ◽

Yeo-Eun Kim ◽

Young Nyun Park ◽

Gi Hong Choi ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Progenitor Cell ◽

Cell Markers ◽

Mri Features

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Influences of Central Bouquet Alterations on the Visual Outcome in Eyes Receiving Epiretinal Membrane Surgery

Applied Sciences ◽

10.3390/app11030926 ◽

2021 ◽

Vol 11 (3) ◽

pp. 926

Author(s):

Max Philipp Brinkmann ◽

Stephan Michels ◽

Carolin Brinkmann ◽

Mario Damiano Toro ◽

Nicole Graf Johansen ◽

...

Keyword(s):

Visual Outcome ◽

Functional Results ◽

Internal Limiting Membrane ◽

Ilm Peeling ◽

Epiretinal Membranes ◽

Staging Systems ◽

Before And After ◽

Pars Plana

Background: Previous studies have shown that epiretinal membranes (ERMs) may be associated with abnormal outer retinal anatomy. However, long-term morphological and functional results of pars plana vitrectomy (PPV) with ERM and internal limiting membrane (ILM) peeling in eyes with central bouquet (CB) alterations have not yet been investigated. Methods: In a retrospective, consecutive study all patients underwent best corrected visual acuity (BCVA) testing and spectral domain optical coherence tomography (SD-OCT) before and after a mean of 20 months (range 3–70 months) postoperatively. CB abnormalities and ERMs were classified according to Govetto’s staging systems. Results: Of the 67 eyes, 22 (34%) showed CB abnormalities at baseline. The mean BCVA increased from 0.42 at baseline to 0.20 LogMAR at final follow-up (p < 0.001). Neither ERM stage (p = 0.06) nor CB stage (p = 0.939) at baseline were significant predictors of vision improvement following surgery. Conclusions: Our results show that baseline BCVA, but not classification of CB changes and ERM at baseline, seems to be a useful predictor for functional outcomes following PPV with ERM and ILM peeling in the long-term.

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Expression of hepatic progenitor cell markers in acute cellular rejection of liver allografts-An immunohistochemical study

Clinical Transplantation ◽

10.1111/ctr.13203 ◽

2018 ◽

Vol 32 (3) ◽

pp. e13203 ◽

Cited By ~ 1

Author(s):

Regina Cheuk-lam Lo ◽

Kristy Kwan-shuen Chan ◽

Carmen Oi-ning Leung ◽

Irene Oi-lin Ng

Keyword(s):

Progenitor Cell ◽

Immunohistochemical Study ◽

Acute Cellular Rejection ◽

Hepatic Progenitor Cell ◽

Cell Markers ◽

Cellular Rejection

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Inflammation increases cells expressing ZSCAN4 and progenitor cell markers in the adult pancreas

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00299.2012 ◽

2013 ◽

Vol 304 (12) ◽

pp. G1103-G1116 ◽

Cited By ~ 7

Author(s):

Shigeru B. H. Ko ◽

Sakiko Azuma ◽

Yukihiro Yokoyama ◽

Akiko Yamamoto ◽

Kazuhiro Kyokane ◽

...

Keyword(s):

Autoimmune Pancreatitis ◽

Genome Stability ◽

Progenitor Cell ◽

Es Cells ◽

Embryonic Stem ◽

Corticosteroid Treatment ◽

Human Pancreas ◽

Cell Markers ◽

Telomere Elongation

We have recently identified the zinc finger and SCAN domain containing 4 (Zscan4), which is transiently expressed and regulates telomere elongation and genome stability in mouse embryonic stem (ES) cells. The aim of this study was to examine the expression of ZSCAN4 in the adult pancreas and elucidate the role of ZSCAN4 in tissue inflammation and subsequent regeneration. The expression of ZSCAN4 and other progenitor or differentiated cell markers in the human pancreas was immunohistochemically examined. Pancreas sections of alcoholic or autoimmune pancreatitis patients before and under maintenance corticosteroid treatment were used in this study. In the adult human pancreas a small number of ZSCAN4-positive (ZSCAN4+) cells are present among cells located in the islets of Langerhans, acini, ducts, and oval-shaped cells. These cells not only express differentiated cell markers for each compartment of the pancreas but also express other tissue stem/progenitor cell markers. Furthermore, the number of ZSCAN4+cells dramatically increased in patients with chronic pancreatitis, especially in the pancreatic tissues of autoimmune pancreatitis actively regenerating under corticosteroid treatment. Interestingly, a number of ZSCAN4+cells in the pancreas of autoimmune pancreatitis returned to the basal level after 1 yr of maintenance corticosteroid treatment. In conclusion, coexpression of progenitor cell markers and differentiated cell markers with ZSCAN4 in each compartment of the pancreas may indicate the presence of facultative progenitors for both exocrine and endocrine cells in the adult pancreas.

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Recurrent anaplastic ganglioglioma: pathological characterization of tumor cells

Journal of Neurosurgery ◽

10.3171/jns.1996.84.6.1055 ◽

1996 ◽

Vol 84 (6) ◽

pp. 1055-1059 ◽

Cited By ~ 38

Author(s):

Atushi Sasaki ◽

Junko Hirato ◽

Yoichi Nakazato ◽

Masaru Tamura ◽

Hirotaka Kadowaki

Keyword(s):

Tumor Cells ◽

Glial Cells ◽

Organizer Region ◽

Nucleolar Organizer Region ◽

Nucleolar Organizer ◽

Left Frontal Lobe ◽

Ki 67 ◽

Content Type ◽

Initial Tumor ◽

Mib 1

✓ A total resection of a left frontal lobe tumor in a 26-year-old man revealed differentiated ganglioglioma with small foci of atypical glial cells exhibiting mild atypia. Six and one-half years later, a large, well-demarcated tumor recurred; at that time, histological analysis revealed both typical ganglioglioma and highly cellular anaplastic areas, the latter predominating. Although the patient subsequently underwent total and subtotal resections, radiation therapy, and chemotherapy, tumors continued to recur at progressively shorter intervals and he died at the age of 35 years. Biopsies of tissue obtained at the last three resections and the autopsy revealed only anaplastic tumor cells. Routine histological examinations indicated that these tumors were uniformly composed of undifferentiated cells. However, pathological studies using immunohistochemical analysis, electron microscopy, and immunoblot analysis demonstrated that a small number of recurrent anaplastic cells had astrocytic features. Results of Ki-67/MIB-1 labeling and silver nucleolar organizer region counts for those cells were high for glial tumors. A retrospective study of the initial tumor showed slightly high MIB-1 labeling for atypical glial cells. This case is characterized by pathological findings of recurrent tumors that correspond to an unusual form of malignant glioma exhibiting slight astrocytic differentiation. The present case suggests that a longer follow-up period (> 5 years) is necessary in cases of ganglioglioma with mild atypia and that careful examinations, including proliferating potential analysis of initial tumor cells, could be important for the diagnosis and treatment of ganglioglioma.

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