scholarly journals Simultaneous Determination of Schisandrin and Promethazine with Its Metabolite in Rat Plasma by HPLC-MS/MS and Its Application to a Pharmacokinetic Study

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Sijia Gao ◽  
Xuelin Zhou ◽  
Liwei Lang ◽  
Honghong Liu ◽  
Jianyu Li ◽  
...  
Keyword(s):  
Drug Interaction ◽  
Simultaneous Determination ◽  
Water Extract ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Interaction Study ◽  
Triple Quadrupole Mass Spectrometer ◽  
Drug Interaction Study ◽  
Limit Of Quantification

This study aimed to develop a selective, simple, and sensitive HPLC-MS/MS method for the simultaneous determination of schisandrin and promethazine (PMZ) with its metabolite in rat plasma, which was further used for a pharmacokinetic herb-drug interaction study. HPLC-MS/MS analyses were performed on an Agilent Technologies 1290 LC and a 6410 triple quadrupole mass spectrometer. The following parameters, the lower limit of quantification (LLOQ), calibration curve, accuracy, precision, stability, matrix effect, and recovery, were validated. The linear range of the developed method for PMZ, its metabolite promethazine sulfoxide (PMZSO), and schisandrin in rat plasma was 0.5–200 ng/mL (R2 > 0.995), with an LLOQ of 0.5 ng/mL, which completely met the determination requirements of biosamples. The intra- and interday precision (RSD, %) was below 13.31% (below 16.67% for the LLOQ) in various plasma, whose accuracy (bias, %) was from −8.52% to 11.40%, which were both within an acceptable range. This method was successfully applied to a pharmacokinetic herb-drug interaction study after oral administration of PMZ with or without S. chinensis water extract. The results demonstrated that coadministration with the S. chinensis water extract might affect the pharmacokinetic behaviors of PMZ. In turn, when taken together with PMZ, the pharmacokinetic parameters of schisandrin, the main active component of S. chinensis, were also affected. The method established in the current study was selective, simple, sensitive, and widely available with good linearity, high accuracy and precision, and a stable sample preparation process. Moreover, this analytical method provides a significant approach for the investigation of herb-drug interaction between S. chinensis and PMZ. The potential pharmacokinetic herb-drug interaction of PMZ- and schisandrin-containing preparations should be noted.

Simultaneous Determination of Methadone, Fluoxetine, Venlafaxine and Their Metabolites in Rat Plasma by UPLC–MS/MS for Drug Interaction Study

2016 ◽  
Vol 79 (9-10) ◽  
pp. 601-608 ◽  
Author(s):  
Pei-Pei Pan ◽  
Shuang-Hu Wang ◽  
Jun Wang ◽  
Jun Luo ◽  
Pei-Wu Geng ◽  
...  
Keyword(s):  
Drug Interaction ◽  
Simultaneous Determination ◽  
Rat Plasma ◽  
Interaction Study ◽  
Drug Interaction Study

High-throughput LC-MS/MS Method for Simultaneous Determination of Pantoprazole and Atorvastatin in Rat Plasma: Application to a Pharmacokinetic Interaction Study

2021 ◽  
Vol 22 ◽  
Author(s):  
Jian Le ◽  
Yuehua Liao ◽  
Shengni Li ◽  
Xiujuan Chen ◽  
Zhanying Hong
Keyword(s):  
Drug Interaction ◽  
Multiple Reaction Monitoring ◽  
Pharmacokinetic Interaction ◽  
Rat Plasma ◽  
Interaction Study ◽  
Triple Quadrupole Mass Spectrometer ◽  
Internal Standards ◽  
Spectrometric Data ◽  
Drug Drug Interaction

Background: Pantoprazole and atorvastatin are often used jointly in the clinic. The drug-drug interaction of pantoprazole and atorvastatin is worthy of being investigated. Objective: A highly rapid, sensitive, and selective LC-MS/MS method was developed for simultaneous quantification of pantoprazole and atorvastatin in rat plasma. Methods: Omeprazole and atorvastatin-d5 were used as the internal standards (ISs) of pantoprazole and atorvastatin, respectively. Simple protein precipitation was used to extract analytes from 50.0 μL plasma samples. Results: The chromatographic separation was achieved on a C18 column and the total chromatographic run time was 3.2 min. Acquisition of mass spectrometric data was performed on a triple-quadrupole mass spectrometer in multiple- reaction-monitoring (MRM) mode with an ESI source using the transition m/z 384→ 200 for pantoprazole and m/z 559.4→ 440.2 for atorvastatin, respectively. The method was validated over the concentration range of 20.0 ∼ 5000 ng/mL for pantoprazole and 1.00 ∼ 250 ng/mL for atorvastatin. All the validation results, including linearity, specificity, precision, accuracy, extraction recovery, matrix effect, and stability, met the acceptance criteria as per FDA guidelines. Conclusion: This method was successfully applied to a pharmacokinetic interaction study in Wistar rats. The results revealed significant evidence for the drug-drug interaction between pantoprazole and atorvastatin.

scholarly journals Simultaneous determination of amoxicillin and chloramphenicol and their drug interaction study by the validated UPLC method

2016 ◽  
Vol 10 (5) ◽  
pp. 755-765 ◽  
Author(s):  
Mohammad Nasir Uddin ◽  
Suman Das ◽  
Shakhawat Hasan Khan ◽  
Swapon Kumar Shill ◽  
Habibur Rahman Bhuiyan ◽  
...  
Keyword(s):  
Drug Interaction ◽  
Simultaneous Determination ◽  
Interaction Study ◽  
Drug Interaction Study

LC–MS/MS method for simultaneous determination of rivaroxaban and metformin in rat plasma: application to pharmacokinetic interaction study

Bioanalysis ◽  
2019 ◽  
Vol 11 (24) ◽  
pp. 2269-2281 ◽  
Author(s):  
Shouchang Gai ◽  
Anli Huang ◽  
Tian Feng ◽  
Nan Gou ◽  
Xingchen Wang ◽  
...  
Keyword(s):  
Drug Interactions ◽  
Simultaneous Determination ◽  
Pharmacokinetic Interaction ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Interaction Study ◽  
Single Group ◽  
Us Fda ◽  
First Time

Aim: A reliable, sensitive and simple LC–MS/MS method has been established and validated for the quantitation of rivaroxaban (RIV) and metformin (MET) in rat plasma. Results: The procedure of method validation was conducted according to the guiding principles of EMA and US FDA. At the same time, the method was applied to pharmacokinetic interactions study between RIV and MET for the first time. When RIV and MET coadministered to rats, pharmacokinetic parameters of MET like AUC(0-t), AUC(0-∞) and Cmax had statistically significant increased. tmax of RIV was prolonged without affecting t1/2 obviously and Cmax was inhibited significantly (p < 0.05) by comparison to the single group. Conclusion: The results indicated that drug–drug interactions occurred when the coadministration of RIV and MET.

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